Anti-beta Actin antibody [AC-15] - Loading Control (Ab6276)

Beta-actin, ACTB

反应种属:Human, Mouse, Rat, Dog, Monkey, Bovine, Hamster
抗原名称:ACTB
应用:WB, IF/ICC

用户验证的应用

推荐稀释比例

  • WB1/5000 - 1/16000
  • ICC/IF5 µg/mL

详细信息

免疫原Synthetic Peptide within Human ACTB aa 1-50 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. P60709
宿主/亚型Mouse / IgG1
抗体类型Monoclonal
全称Anti-beta Actin antibody [AC-15] - Loading Control
产品形式Liquid
储存缓冲液pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS
储存条件运输条件: Blue Ice 推荐的短期储存时间: 1-2 weeks 推荐的短期储存条件: +4°C 推荐的长期储存条件: -20°C 储存信息: Avoid freeze / thaw cycle
纯化方式Affinity purification

背景信息

Anti-beta Actin antibody [AC-15] (ab6276) is a mouse monoclonal antibody and is validated for use in ICC/IF and WB. Anti-beta Actin antibody [AC-15] (ab6276) was first used in a scientific publication in 1994 and has been cited over 2378 times in peer reviewed journals. It's performance in Western blot in human, mouse and rat samples is trusted by the scientific community. Abcam's high quality validation processes ensure Anti-beta Actin antibody [AC-15] (ab6276) has high sensitivity and specificity. The specificity of Anti-beta Actin antibody [AC-15] (ab6276) has been confirmed by WB testing in beta Actin knockout HAP1 cells. Anti-beta Actin antibody [AC-15] (ab6276) has 94 independent reviews from customers. beta Actin antibodies are often used as loading controls in Western Blot. Anti-beta Actin antibody [AC-15] has been verified in Western Blot samples and detects a band at 42kDa Molecular weight. Anti-beta Actin antibody [AC-15] (ab6276) specifically detects beta Actin (UniProt ID: P60709; Molecular weight: 42kDa) and is sold in 50 µL selling sizes. Antibody clone AC-15 is also available pre-conjugated to a variety of labels for your convenience - HRP, FITC ( ab499, ab6277 ). Beta-Actin (β-actin) is a highly conserved protein that plays a crucial role in cell motility, structure and integrity. It is one of the six actin isoforms found in humans and is a major component of the cytoskeleton in non-muscle cells. β-actin is involved in various cellular processes, including cell division, signaling and maintaining cell shape. Due to its ubiquitous expression and stability, β-actin is commonly used as a loading control in western blotting (WB) and other protein quantification assays. Understanding β-actin's functions and interactions is essential for studying cellular dynamics and various physiological processes.

文献引用 (1979)

Long-range chemical signalling in vivo is regulated by mechanical signals.

体内长程化学信号传导受机械信号调控。

Pillai Eva K, Mukherjee Sudipta, Gampl Niklas, McGinn Ross J, Mooslehner Katrin A, Becker Julia M, Winkel Alexander K, Thompson Amelia J, Franze Kristian

Targeting VEGFR2 inhibition within a spatially-confined conduit promotes nerve self-resolution and alleviates mechanical allodynia.

在空间受限的导管内靶向抑制 VEGFR2 可促进神经自我修复并缓解机械性痛觉过敏。

Luo Xiaobin, Nagayasu Toshitatsu, Zhang Yuhao, He Zhi, Yang Jia, Li Chengli, Duan Guman, Zong Qunchuan, Liu Yun, Yang Kaiyuan, Mao Kaige, Ma Pengchao, Li Zifan, Zhao Yating, Kim KunKoo, Zhang Jie, Wang Zide, Luo Yuyang, Lu Jingsong, Tong Sengpav, Yu Jia-Kuo, Zhao Zhe, Cao Zheng, Yang Shuhui, Pan Yongwei, Wang Xiumei

The transcription factor EHF promotes the maturation and immunosuppression of conventional dendritic cells.

转录因子 EHF 促进常规树突状细胞的成熟和免疫抑制。

Liu Xiaoli, Wang Ling, Xiao Yongfang, Ni Hai, Huang Jiancheng, Yao Kai, Zhao Wei, Yang Jian-Rong, Zhao Jijun, Wu Angela R, Yang Cliff Y

REEP1 Accumulation Disrupts ER Integrity and Drives Spinal Motoneuron Degeneration in Distal Hereditary Motor Neuropathy.

REEP1 积累破坏内质网完整性,导致远端遗传性运动神经病脊髓运动神经元退化。

Bock Andrea, Schurig Mona, Willoughby Miles, Mirecki Andrea, Seemann Eric, Lohachova Kateryna, Katona Istvan, Mittag Sonnhild, Liebmann Lutz, Franzka Patricia, Heidari Horestani Mehdi, Khundadze Mukhran, Mosler Thorsten, Louie Timothy, de Visser Marianne, Weterman Marian A J, Kiehntopf Michael, Beetz Christian, Nietzsche Sandor, Huber Otmar, Weis Joachim, Kessels Michael M, Bhaskara Ramachandra M, Qualmann Britta, Đikić Ivan, Hübner Christian A

ER-associated degradation pathway protein SEL1L plays an evolutionarily conserved role in platelet adhesion.

内质网相关降解途径蛋白 SEL1L 在血小板粘附中发挥着进化保守的作用。

Dahlgren Anna R, Careddu Francesca, Norris Jeffrey W, Di Buduo Christian A, Stanger Livia, Adili Reheman, Kropp Erin M, Li Qing, Holinstat Michael, Biunno Ida, Balduini Alessandra, Tablin Fern, Shavit Jordan A, Finno Carrie J

Specific SLC25 carriers regulate mitochondrial protein synthesis.

特定的SLC25载体调节线粒体蛋白质合成。

Rudler Danielle L, Hughes Laetitia A, King Martin S, Baker Jessica, Lee Richard G, Gandadireja Andrianto P, Sunil Anisha, Fagan Samuel V, Payne Blake, Gray Nicola, McCubbin Tim, Kunji Edmund R S, Rackham Oliver, Filipovska Aleksandra

CAR-T triggers TAM reeducation and adaptive anti-tumor response via TREM2 deficiency or CD40 agonist.

CAR-T 通过 TREM2 缺陷或 CD40 激动剂触发 TAM 再教育和适应性抗肿瘤反应。

Liu Ting, Gao Huixin, Xi Zhihui, Yu TianTian, Gu Yimei, Mai Hanbing, Yuan Hui, Liu Yafang, Liu Haikuan, Zhang Qiaoxuan, Huang Xianzhang, Fan Wenzhe, Tan Jizhou

The chaperonin TRiC component Cct3 is required for axonal transport, myelination, and neuromuscular junction refinement.

伴侣蛋白 TRiC 成分 Cct3 是轴突运输、髓鞘形成和神经肌肉接头精细化所必需的。

Zhang Xiaomeng, Zajt Kamil Kajetan, Palaz Tayfun, Wang Lisa, Groß Martin, Kraft Florian, Weis Joachim, Bremer Juliane

mTOR-driven autophagy suppression defines metabolic vulnerability in CDK4/6 inhibitor-resistant HR(+)/HER2(-) breast cancer.

mTOR 驱动的自噬抑制定义了 CDK4/6 抑制剂耐药的 HR(+)/HER2(-) 乳腺癌的代谢脆弱性。

von Wichert Luise, Stroh Alina, Witt Marie, Wanzel Michael, Mernberger Marco, Griewing Sebastian, Wündisch Thomas, Pfitzner Berit M, Teply-Szymanski Julia, Litmeyer Anne-Sophie, Denkert Carsten, Wagner Uwe, Stiewe Thorsten, Gremke Niklas

Targeting the p53 cancer mutants Y220C, Y220N, and Y220S with the small-molecule stabilizer rezatapopt.

使用小分子稳定剂rezatapopt靶向p53癌症突变体Y220C、Y220N和Y220S。

Mavridi Danai, Funk Julianne S, Balourdas Dimitrios-Ilias, Krämer Andreas, Khan Tareque Raysa, Timofeev Oleg, Spencer John, Stiewe Thorsten, Joerger Andreas C
  • 1
  • 2
  • 3
  • 4
  • 5
  • 6
  • 198