Anti-APG5L/ATG5 antibody [EPR1755(2)] (Ab108327)

APG5L, ASP, ATG5, Autophagy protein 5, APG5-like, Apoptosis-specific protein

反应种属:Human, Mouse, Rat
抗原名称:ATG5
应用:WB, IHC, IF/ICC, IP

用户验证的应用

推荐稀释比例

  • IHC-P1/100 - 1/250 Antigen retrieval is recommended. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • WB1/1000 - 1/10000
  • ICC/IF1/100 - 1/250
  • IP1/10 - 1/100

详细信息

免疫原The exact immunogen used to generate this antibody is proprietary information.
宿主/亚型Rabbit / IgG
抗体类型Monoclonal
全称Anti-APG5L/ATG5 antibody [EPR1755(2)]
产品形式Liquid
储存缓冲液pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
储存条件运输条件: Conditional Ambient 推荐的短期储存时间: 1-2 weeks 推荐的短期储存条件: +4°C 推荐的长期储存条件: -20°C 储存信息: Stable for 12 months at -20°C
纯化方式Affinity purification Protein A

背景信息

What is this antibody validated in? Anti-APG5L/ATG5 antibody [EPR1755(2)] (ab108327) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples. What is the molecular weight of APG5L/ATG5? Anti-APG5L/ATG5 [EPR1755(2)] (ab108327) specifically detects a band for APG5L/ATG5 (UniProt: Q9H1Y0) at a molecular weight of 32kDa. Trusted by the scientific community Anti-APG5L/ATG5 [EPR1755(2)] (ab108327) was first used in a scientific publication in 2012 and has been cited over 190 times in peer-reviewed journals. Reviewed by scientists Anti-APG5L/ATG5 [EPR1755(2)] (ab108327) has over 5 independent reviews from customers. Trial sizes available! Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies. Specificity confirmed The specificity of Anti-APG5L/ATG5 antibody [EPR1755(2)] (ab108327) has been confirmed by Western blot testing in ATG5 Knockout HAP1 cell line, ab277835. Other related products We have a range of other formats of antibody clone [EPR1755(2)] also available for your convenience: ab108327, HRP - ab199560, Carrier free - ab221604, ab307842, Carrier free - ab307843 Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents. What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies.

文献引用 (258)

Comprehensive methodological evaluation of V-ATPase assembly in the context of cardiac lipid overload: implications for (endo)lysosomal function and autophagy.

对心脏脂质过载背景下 V-ATPase 组装的综合方法学评价:对(内)溶酶体功能和自噬的影响。

Tie Hongtao, Hou Mengqian, Zhang Jun, Hoes Martijn F, Neumann Dietbert, Luiken Joost J F P, Wang Shujin

ESRRA-ATG5-Mediated mitophagy enhances arginine metabolism to alleviate diabetic kidney disease.

ESRRA-ATG5介导的线粒体自噬增强精氨酸代谢,从而缓解糖尿病肾病。

Hu Hongtu, Yang Qian, Hu Jijia, Fan Yanqing, Zhang Zongwei, Yang Keju, Li Weiwei, Peng Zhuan, Chen Zhaowei, Ding Guohua, Liang Wei

Osteoarthritis pathogenesis Unveiled: DMT1 drives autophagy-dependent ferroptosis through a mir-17-5p/NEDD4 regulatory axis.

骨关节炎发病机制揭秘:DMT1 通过 mir-17-5p/NEDD4 调控轴驱动自噬依赖性铁死亡。

Exploring the effects of lifelong aerobic exercise on early skeletal muscle remodeling between 8 and 14 months in the rat.

探索终生有氧运动对 8 至 14 个月大大鼠早期骨骼肌重塑的影响。

Moreira-Pais Alexandra, Ferreira Rita, Neuparth Maria João, Fardilha Margarida, Moreira-Gonçalves Daniel, Oliveira Paula A, Duarte José A

CBX2 promotes cisplatin resistance in ovarian cancer via SIAH2-mediated β-catenin stabilization and ATG9B-dependent autophagy activation.

CBX2 通过 SIAH2 介导的 β-catenin 稳定和 ATG9B 依赖的自噬激活促进卵巢癌的顺铂耐药性。

Increasing autophagy activity suppresses Helicobacter pylori infection-related gastric cancer tumorigenesis both in vitro and in vivo.

提高自噬活性可抑制幽门螺杆菌感染相关的胃癌在体外和体内的肿瘤发生。

Lin Pei-Wen, Liu Yu-Wen, Chu Man-Ling, Chen Yu-Cing, Shih Yao-Hsiang, Ma Yu-Chun, Lin Pin-Yuan, Kuo I-Ying, Chang Hong-Yi, Chen Li-Tzong, Wang Jaw-Yuan, Lee Ying-Ray, Kuo Chao-Hung, Wu Deng-Chyang, Liu Hsiao-Sheng

Oleanolic acid-chitosan compound inhibits mitochondrial autophagy and malignant transformation of lung cancer through the PTEN/AKT pathway.

齐墩果酸-壳聚糖化合物通过 PTEN/AKT 通路抑制线粒体自噬和肺癌的恶性转化。

Abulaiti Abulimiti, Sun XiaoHong, Yibulayin Waresijiang, He Dan, Xu KeMing, Yibulayin Xiayimaierdan

Structural organization of p62 filaments and the cellular ultrastructure of calcium-rich p62-enwrapped lipid droplet cargo.

p62丝的结构组织和富含钙的p62包裹的脂滴货物的细胞超微结构。

Berkamp Sabrina, Jungbluth Lisa, Katranidis Alexandros, Mostafavi Siavash, Korculanin Olivera, Lu Peng-Han, Ickert Lotte, Dierig Maya M, Sharma Lokesh, Thukral Lipi, Huesgen Pitter F, Kononenko Natalia L, Fitter Jörg, Dunin-Borkowski Rafal E, Sachse Carsten

TANGO2 binds crystallin alpha B and its loss causes desminopathy.

TANGO2 与晶状体蛋白 α B 结合,其缺失会导致结蛋白病

Stentenbach Maike, Hughes Laetitia A, Fagan Samuel V, Payne Blake, Rudler Danielle L, Siira Stefan J, McCubbin Tim, Chopin Anaëlle, Perks Kara L, Ermer Judith A, Hendry James, Er Teagan S, Balasubramaniam Shanti, Eliades Joel A, Hool Livia C, Packer Nicolle H, Moh Edward S X, Padman Benjamin S, Rackham Oliver, Filipovska Aleksandra

Macrophage autophagy protects against acute kidney injury by inhibiting renal inflammation through the degradation of TARM1

巨噬细胞自噬通过降解TARM1抑制肾脏炎症,从而起到保护肾脏免受急性肾损伤的作用。

Xiao-Rong Huang ,Lin Ye ,Ning An ,Chun-Yu Wu ,Hong-Luan Wu ,Hui-Yuan Li ,Yan-Heng Huang ,Qiao-Ru Ye ,Ming-Dong Liu ,La-Wei Yang ,Jian-Xing Liu ,Ji-Xin Tang ,Qing-Jun Pan ,Peng Wang ,Lin Sun ,Yin Xia ,Hui-Yao Lan ,Chen Yang ,Hua-Feng Liu
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