Anti-Nanog antibody [EPR2027(2)] (Ab109250)

Homeobox protein NANOG, Homeobox transcription factor Nanog, hNanog, NANOG

反应种属:Human
抗原名称:Nanog
应用:WB, IHC, FCM, IF/ICC, IP, ChIP-seq, CUT&RUN-seq

用户验证的应用

推荐稀释比例

  • IHC-P1/100 - 1/250 Antigen retrieval is recommended. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • WB1/1000 - 1/10000
  • ICC/IF1/100 - 1/250
  • Flow Cyt (Intra)1/70 ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • IP1/40
  • ChIC/CUT&RUN-seq5 µg

详细信息

免疫原The exact immunogen used to generate this antibody is proprietary information.
宿主/亚型Rabbit / IgG
抗体类型Monoclonal
全称Anti-Nanog antibody [EPR2027(2)]
产品形式Liquid
储存缓冲液pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
储存条件运输条件: Conditional Ambient 推荐的短期储存时间: 1-2 weeks 推荐的短期储存条件: +4°C 推荐的长期储存条件: -20°C 储存信息: Avoid freeze / thaw cycle
纯化方式Affinity purification Protein A

背景信息

What is this antibody validated in? Anti-Nanog antibody [EPR2027(2)] (ab109250) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF), ChIP in Human samples. What is the molecular weight of Nanog? Anti-Nanog [EPR2027(2)] (ab109250) specifically detects a band for Nanog (UniProt: Q9H9S0) at a molecular weight of 35kDa. Trusted by the scientific community Anti-Nanog [EPR2027(2)] (ab109250) was first used in a scientific publication in 2011 and has been cited over 190 times in peer-reviewed journals. Reviewed by scientists Anti-Nanog [EPR2027(2)] (ab109250) has over 10 independent reviews from customers. Trial sizes available! Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies. Other related products We have a range of other formats of antibody clone [EPR2027(2)] also available for your convenience: ab109250, Alexa Fluor® 647 - ab195018, Alexa Fluor® 488 - ab196155, Carrier free - ab218524 Species reactivity Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information. Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents. What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies.

文献引用 (246)

Inhibition of FOS-Like Antigen 1 Reduces Chemoresistance to Temozolomide Through Stemness Reprogramming via IL-6/STAT3(Tyr705) Pathway.

抑制 FOS 样抗原 1 通过 IL-6/STAT3(Tyr705) 通路进行干性重编程,从而降低对替莫唑胺的化疗耐药性。

Sim JeongMin, Park JeongMan, Heo JinHyung, Kim Yu Jin, Kim Dongkil, Jeon Yong Hyun, Lee Jae-Eon, Choi Seon Hee, Kim Hak Jin, Hwang So Jung, Kim Junhyung, Cho Kyung Gi, Yoo Jihwan, Moon Ju Hyung, Moon Jong-Seok, Sung Kyoung Su, Lim Jaejoon

Brain capillary endothelial-like cells show altered barrier functionality and reduced transport of amyloid β in late-onset Alzheimer disease.

晚发性阿尔茨海默病中,脑毛细血管内皮样细胞表现出屏障功能改变和淀粉样蛋白β转运减少。

Hartmann Carla, Haferkamp Undine, Appelt-Menzel Antje, Barenberg Janica, Brachner Andreas, Ehrhard Toni, Feldhaus Julia, Gerhartl Anna, Hollemann Thomas, Kulka Linda Anna Michelle, Leckzik Selin, Leu Jennifer, Woo Marcel Seungsu, Friese Manuel Alexander, Initiative Alzheimer's Disease Neuroimaging, Metzger Marco, Neuhaus Winfried, Oerter Sabrina, Olzscha Heidi, Pich Andreas, Riemann Dagmar, Pless Ole, Rujescu Dan, Jung Matthias

APOE4 drives widespread changes to the hepatic proteome and alters metabolic function.

APOE4 驱动肝脏蛋白质组发生广泛变化,并改变代谢功能。

Lysaker Colton R, Johnson Chelsea N, Csikos Vivien, Franczak Edziu, Tetlow Ashley L, Benson Maggie, Thyfault John P, Geiger Paige C, Morris Jill K, Wilkins Heather M

Generation of an isogenic human induced pluripotent stem cell line with a mutant propionyl-CoA carboxylase α subunit.

构建具有突变丙酰辅酶A羧化酶α亚基的同源人类诱导多能干细胞系。

Generation of induced pluripotent stem cells from a patient with CHARGE syndrome with athymia, harboring a heterozygous mutation in CHD7.

从患有 CHARGE 综合征且无胸腺的患者体内生成诱导多能干细胞,该患者携带 CHD7 杂合突变。

Zhao Jin, Hu Rong, Lai Kuan Chen, Liu Yaling, Carmichael Gordon G, Martin Donna M, Lai Laijun

Expression of pluripotency stem cell markers in endometrial hyperplasia.

子宫内膜增生中多能干细胞标志物的表达。

Mousa Noha A, Al-Rawi Zeinab, Hussein Amal, Suliman Abdalla, Mohammed Ghada, Hamoudi Rifat, Al-Naimi Suha

Engineering mtDNA deletions by reconstituting end joining in human mitochondria

通过在人类线粒体中重建末端连接来构建线粒体DNA缺失

Yi Fu ,Max Land ,Tamar Kavlashvili ,Ruobing Cui ,Minsoo Kim ,Emily DeBitetto ,Toby Lieber ,Keun Woo Ryu ,Elim Choi ,Ignas Masilionis ,Rahul Saha ,Meril Takizawa ,Daphne Baker ,Marco Tigano ,Caleb A Lareau ,Ed Reznik ,Roshan Sharma ,Ronan Chaligne ,Craig B Thompson ,Dana Pe'er ,Agnel Sfeir

Cell-type-specific functionality encoded within the intrinsically disordered regions of OCT4

OCT4固有无序区编码的细胞类型特异性功能

Burak Ozkan #,Mitzy Rios de Anda #,Elisa Hall-Ponsele,Maria Rosa Portero Migueles,Amani Alshaikh,Marta Hanzevacki,Moriyah Naama,Katharine Furlong,Gareth A Roberts,Meryam Beniazza,My Linh Huynh,Michael R O'Dwyer,Sonia Yiakoumi,Christos Spanos,Hazar Yassen,Keisuke Kaji,Hitoshi Niwa,Yosef Buganim,Sally Lowell,Abdenour Soufi

Pulsatile sequential drug release system for cascade tumor deep penetration and differentiation therapy to enhance chemoimmunotherapy.

脉冲式顺序药物释放系统,用于级联肿瘤深层渗透和分化治疗,以增强化疗免疫疗法

Liu Fengxiang, Ning Shipeng, Wang Xia, Fan Xiaoyuan, Wan Bin, Sun Fei, Du Lili, Shi Kefan, Zou Xinpeng, Zhu Ruihong, Li Mingxing, Shen Wenwen, He Zhonggui, Wang Kaiyuan, Sun Jin

3D printing-biomimetic local stiff niche enhances glycolysis to boost PDAC cell stem-like phenotype via N6-methyladenosine-suppressed YAP1 mRNA decay.

3D 打印仿生局部刚性微环境通过 N6-甲基腺苷抑制 YAP1 mRNA 降解来增强糖酵解,从而促进 PDAC 细胞干细胞样表型

Wu Di, Guan Xiaoqi, Yang Tao, Yan Jiashuai, Zhu Biwen, Zhou Junchao, Guo Yibing, Lu Yuhua
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