Anti-alpha Tubulin antibody [DM1A] - Loading Control (Ab7291)

推荐稀释比例

• IHC-P5 µg/mL Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
• WB1/5000 - 1/10000 We recommend diluting ab7291 to 1:10000 and incubating overnight at 4°C. Works under both reducing and non-reducing conditions. We recommend using 3% BSA as the blocking agent, blocking with milk may cause a reduction in signal intensity.
• ICC/IF0.5-1 µg/mL
• Flow Cyt (Intra)1 µg for 10^6 Cells ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) is a mouse monoclonal antibody and is validated for use in Flow Cyt (Intra), ICC/IF, IHC-P and WB. Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) was first used in a scientific publication in 2005 and has been cited over 1224 times in peer reviewed journals. It's performance in Western blot in human, mouse and rat samples is trusted by the scientific community. Abcam's high quality validation processes ensure Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) has high sensitivity and specificity. Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) has 91 independent reviews from customers. alpha Tubulin antibodies are often used as loading controls in Western Blot. Anti-alpha Tubulin antibody [DM1A] - Loading Control has been verified in Western Blot samples and detects a band at 51kDa Molecular weight. Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) specifically detects alpha Tubulin (UniProt ID: P02552; Molecular weight: 51kDa) and is sold in 100 µg and 250 µg selling sizes. Conjugation-ready, carrier free format available for antibody clone DM1A - ab264493. Antibody clone DM1A is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor ® 68, Alexa Fluor ® 79, Alexa Fluor ® 488, Alexa Fluor ® 594, HRP, FITC ( ab18493, ab184577, ab195887, ab195889, ab4742 ). Alpha-Tubulin (α-Tubulin, TUBBA) is a fundamental protein that forms microtubules, essential components of the cytoskeleton in eukaryotic cells. Microtubules, composed of α- and β-tubulin heterodimers, play a crucial role in maintaining cell shape, enabling intracellular transport and facilitating cell division. Due to its ubiquitous presence and stability, α-Tubulin is commonly used as a loading control in western blotting (WB) and other protein quantification assays. Understanding α-Tubulin's functions and interactions is vital for studying cellular dynamics, mitosis and various physiological processes