Anti-Histone H3 (di methyl K4) antibody - ChIP Grade (Ab7766)

H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, H3C1, H3FC, HIST1H3C, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3K4me2, H3K4me, H3K4

反应种属:Human, Mouse, Rat, Bovine
抗原名称:H3C1 di methyl K4
应用:WB, IF/ICC, ChIP, Peptide Array

详细信息

免疫原The exact immunogen used to generate this antibody is proprietary information.
宿主/亚型Rabbit / IgG
抗体类型Polyclonal
产品形式Liquid
纯化方式Affinity purification Immunogen
储存缓冲液pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA
储存条件运输条件: Blue Ice 推荐的短期储存时间: 1-2 weeks 推荐的短期储存条件: +4°C 推荐的长期储存条件: -20°C 储存信息: Avoid freeze / thaw cycle

特异性

From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help. You may also be interested in our alternative recombinant antibody, ab176878.

背景信息

For detection of Histone H3 specifically methylated at position Lys 4. This antibody was used in a screen by Dover et al, (2002) to isolate yeast mutants that are unable to methylate Lysine 4. In immunofluorescence, this antibody detects foci in the nucleus that are non-colocalising with condensed chromatin. The perinuclear and perinucleolar heterochromatin are not stained with this antibody. Learn about ChIP assay kits, other ChIP antibodies, protocols and more in the ChIP assay guide.

文献引用 (256)

A SET domain-containing protein and HCF-1 maintain transgenerational epigenetic memory.

SET结构域蛋白和HCF-1维持跨代表观遗传记忆。

Inhibiting LSD1 unlocks retinoid AP-1 programming to activate epithelial immunity and skin tumor suppression.

抑制 LSD1 可解锁类视黄醇 AP-1 程序,从而激活上皮免疫和皮肤肿瘤抑制。

Kuprasertkul Nina, Moore Alyssa F, D'souza Carina A, Chini Julia, Ko Eun-Kyung, Huang Sijia, Zhang Shuo, Anderson Ashley S, Egolf Shaun, Pinheiro Laura V, Jaccard Alison, Magahis Claudia T, Bao Lydia, Aubert Yann, Olingou Cyria, Prouty Stephen M, Brennan-Crispi Donna, Hill David A, Seykora John T, Wellen Kathryn E, Capell Brian C

PRMT1-mediated methylation regulates MLL2 stability and gene expression

PRMT1介导的甲基化调控MLL2的稳定性和基因表达

Dongju An,Jihyun Kim,Byul Moon,Hyoungmin Kim,Hoa Nguyen,Sunghu Park,J Eugene Lee,Jung-Ae Kim,Jaehoon Kim

A histone-centric multi-omics study shows that increased H3K4 methylation sustains triple-negative breast cancer phenotypes.

一项以组蛋白为中心的多组学研究表明,H3K4 甲基化增加可维持三阴性乳腺癌表型。

Noberini Roberta, Robusti Giulia, Vai Alessandro, Savoia Evelyn Oliva, Jodice Maria Giovanna, Bertalot Giovanni, Çat Betül, Pallavicini Isabella, Bonizzi Giuseppina, Capra Maria, Sangalli Claudia Anna, Zambelli Federico, Fusco Nicola, Pece Salvatore, Pavesi Giulio, Minucci Saverio, Bonaldi Tiziana

Kinetochore mutations and histone phosphorylation pattern changes accompany holo- and macro-monocentromere evolution.

着丝粒突变和组蛋白磷酸化模式变化伴随着全着丝粒和巨着丝粒的进化。

Kuo Yi-Tzu, Neumann Pavel, Chen Jianyong, Fuchs Jörg, Schubert Veit, Kumke Katrin, Sader Mariela Analia, Melzer Michael, Zhu Zihao, Himmelbach Axel, Hentrich Heiko, Macas Jiří, Houben Andreas

The phenylalanine-and-glycine repeats of NUP98 oncofusions form condensates that selectively partition transcriptional coactivators

NUP98 癌基因融合蛋白中的苯丙氨酸和甘氨酸重复序列形成凝聚体,选择性地分隔转录共激活因子。

Jeong Hyun Ahn,Yiran Guo,Heankel Lyons,Samuel G Mackintosh,Benjamin K Lau,Ricky D Edmondson,Stephanie D Byrum,Aaron J Storey,Alan J Tackett,Ling Cai,Benjamin R Sabari,Gang Greg Wang

Multi-dimensional epigenomic dynamics converge on H3K4-mediated regulation of low-CO(2) adaptation in Nannochloropsis oceanica.

多维表观基因组动力学汇聚于 H3K4 介导的海洋微拟球藻低 CO(2) 适应的调控。

Gong Yanhai, Wang Qintao, Wei Li, Wang Lianhong, Lv Nana, Du Xuefeng, Shen Chen, Xin Yi, Sun Luyang, Xu Jian

Ligand-specific regulation of a binary enhancer code dictating cellular senescence.

配体特异性调控决定细胞衰老的二元增强子密码

Suter Thomas, Friedman Meyer J, Tazearslan Cagdas, Merkurjev Daria, Ohgi Kenny, Meluzzi Dario, Rosenfeld Michael G, Suh Yousin

KMT2C/KMT2D-dependent H3K4me1 mediates changes in DNA replication timing and origin activity during a cell fate transition

KMT2C/KMT2D依赖的H3K4me1介导细胞命运转变过程中DNA复制时间和复制起始位点活性的变化

Deniz Gökbuget ,Liana Goehring ,Ryan M Boileau ,Kayla Lenshoek ,Tony T Huang ,Robert Blelloch

Coordinated neuron-specific splicing events restrict nucleosome engagement of the LSD1 histone demethylase complex

协调的神经元特异性剪接事件限制了 LSD1 组蛋白去甲基化酶复合物的核小体参与

Robert S Porter, Sojin An, Maria C Gavilan, Masayoshi Nagai, Yumie Murata-Nakamura, Bo Zhou, Katherine M Bonefas, Olivier Dionne, Jeru Manoj Manuel, Joannie St-Germain, Suzanne Gascon, Jacqueline Kim, Liam Browning, Benoit Laurent, Uhn-Soo Cho, Shigeki Iwase
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