Anti-p53 antibody [DO-1] (Ab1101)

特异性

This antibody clone recognises both wild-type and mutant forms of p53 in human samples. It is not designed to recognise any specific p53 mutation. We have confirmed this experimentally and have been able to detect p53 in different cell lines using various applications and treatments. Important note: p53 expression levels vary greatly between cell lines. It has been reported that p53 mutations render the protein more stable, hence mutated cell lines often express higher levels of the p53 protein compared to wild-type cell lines. For low expressing wild type cell lines, p53 expression can be increased with cell treatments such as camptothecin or irinotecan.

What is this antibody validated in? Anti-p53 antibody [DO-1] (ab1101) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples. What is the molecular weight of p53? Anti-p53 [DO-1] (ab1101) specifically detects a band for p53 (UniProt: P04637) at a molecular weight of 43kDa. Trusted by the scientific community Anti-p53 [DO-1] (ab1101) was first used in a scientific publication in 2002 and has been cited over 160 times in peer-reviewed journals. Reviewed by scientists Anti-p53 [DO-1] (ab1101) has over 10 independent reviews from customers. Specificity confirmed The specificity of Anti-p53 antibody [DO-1] (ab1101) has been confirmed by Western blot testing in TP53 Knockout HAP1 cells. Other related products We have a range of other formats of antibody clone [DO-1] also available for your convenience: ab1101, HRP - ab204452, Carrier free - ab237976 Want a custom formulation? This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com