Anti-beta Actin antibody [SP124] - Cytoskeleton Marker (Ab115777)

Beta-actin, ACTB

反应种属:Human, Mouse, Rat
抗原名称:ACTB
应用:WB, IHC, FCM, IF/ICC

用户验证的应用

推荐稀释比例

  • IHC-P1/200 Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • WB1/200
  • ICC/IF1/50
  • Flow Cyt (Intra)1/200 ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

详细信息

免疫原The exact immunogen used to generate this antibody is proprietary information.
宿主/亚型Rabbit / IgG
抗体类型Monoclonal
全称Anti-beta Actin antibody [SP124] - Cytoskeleton Marker
产品形式Liquid
储存缓冲液pH: 7.6 Preservative: 0.1% Sodium azide Constituents: PBS, 1% BSA
储存条件运输条件: Conditional Ambient 推荐的短期储存条件: +4°C 推荐的长期储存条件: -20°C 储存信息: Avoid freeze / thaw cycle
纯化方式Affinity purification Protein A/G

背景信息

What is this antibody validated in? Anti-beta Actin antibody [SP124] - Cytoskeleton Marker (ab115777) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples. What is the molecular weight of beta Actin? Anti-beta Actin [SP124] - Cytoskeleton Marker (ab115777) specifically detects a band for beta Actin (UniProt: P60709) at a molecular weight of 42kDa. Collaboration Anti-beta Actin [SP124] - Cytoskeleton Marker (ab115777) is a clone from the portfolio of Spring Bioscience (Roche) SP clones which have been optimised for immunohistochemistry (IHC). Trusted by the scientific community Anti-beta Actin [SP124] - Cytoskeleton Marker (ab115777) was first used in a scientific publication in 2011 and has been cited over 140 times in peer-reviewed journals. Other related products We have a range of other formats of antibody clone [SP124] also available for your convenience: ab115777, Carrier free - ab242387, Alexa Fluor® 488 - ab267526, ab279418, ab317794, Carrier free - ab317804 What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies.

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