Anti-beta Actin antibody [mAbcam 8224] - Loading Control (Ab8224)

Beta-actin, ACTB

反应种属:Human, Mouse, Rat, Drosophila, Yeast, Xenopus laevis
抗原名称:ACTB
应用:WB, IHC, IF/ICC

用户验证的应用

推荐稀释比例

  • IHC-P1 µg/mL Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • WB1 µg/mL This antibody has been designed for use as a loading control and is ideal for this purpose. Block membrane for 1 hr in 5%BSA. Incubate antibody in TBST for one hour or more.
  • ICC/IF1 µg/mL

详细信息

免疫原The exact immunogen used to generate this antibody is proprietary information.
宿主/亚型Mouse / IgG1
抗体类型Monoclonal
全称Anti-beta Actin antibody [mAbcam 8224] - Loading Control
产品形式Liquid
储存缓冲液pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 6.97% L-Arginine
储存条件运输条件: Blue Ice 推荐的短期储存时间: 1-2 weeks 推荐的短期储存条件: +4°C 推荐的长期储存条件: -20°C 储存信息: Avoid freeze / thaw cycle
纯化方式Affinity purification

背景信息

Anti-beta Actin antibody [mAbcam 8224] - Loading Control (ab8224) is a mouse monoclonal antibody and is validated for use in ICC/IF, IHC-P, WB in human, mouse, rat samples. Anti-beta Actin antibody [mAbcam 8224] - Loading Control (ab8224) has been cited over 526 times in peer reviewed journals and is trusted by the scientific community. Abcam's high quality validation processes ensure Anti-beta Actin antibody [mAbcam 8224] - Loading Control (ab8224) has high sensitivity and specificity. Anti-beta Actin antibody [mAbcam 8224] - Loading Control (ab8224) has 35 independent reviews from customers. Anti-beta Actin antibody [mAbcam 8224] - Loading Control (ab8224) specifically detects beta Actin (UniProt ID: P60709; Molecular weight: 42kDa) and is sold in 100 µg and 250 µg selling sizes. Conjugation-ready, carrier free format available for antibody clone mAbcam 8224 - ab178787. Antibody clone mAbcam 8224 is also available pre-conjugated to a variety of labels for your convenience - HRP ( ab197277 ). Beta Actin (encoded by the ACTB gene) is a crucial component of the cytoskeleton, playing a vital role in cell motility and gene expression. It is commonly used as a beta actin loading control in various experiments to ensure accurate results. Abnormalities in beta Actin are linked to diseases such as cancer and Baraitser-Winter syndrome. Our ACTB antibody are valuable tools for studying these pathways and understanding the molecular mechanisms involved in these conditions. This monoclonal antibody to beta actin works well as a protein loading control in Western blot for a broad range of species including Xenopus, Drosophila and S. pombe. Want a custom formulation? This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

文献引用 (529)

ER-tethering directs TREX1 penetration of a BAF-dependent barrier at micronuclei.

内质网锚定引导 TREX1 穿透微核处 BAF 依赖性屏障。

Chen Yanyang, Norman Roshan Xavier, Toufektchan Eléonore, Luan Xiaohan, Shim Abraham, Rosenberg Hannah, Kovacs Marton Tibor, Nichols Ashley, Hickling James, Cifani Paolo, Kentsis Alex, Zhou Wen, Maciejowski John

Mechanistic insights into PCBP1-driven unfolding of selected i-motif DNA at G(1)/S checkpoint.

从机制上深入了解 PCBP1 驱动的 G(1)/S 检查点特定 i-motif DNA 的展开。

Sengupta Pallabi, Gillet Natacha, Obi Ikenna, Sabouri Nasim

Human FUS is toxic via association with RNA polymerase II in Drosophila.

人类 FUS 通过与果蝇 RNA 聚合酶 II 结合而产生毒性。

Moens Thomas G, Biasetti Luca, Scheveneels Wendy, Smith Bradley N, Troakes Claire, Van Damme Philip, Vance Caroline, Van Den Bosch Ludo

Platelet-like particles released from Ebola virus-infected megakaryocytic cells behave like virus-like particles.

从埃博拉病毒感染的巨核细胞中释放出的血小板样颗粒表现得像病毒样颗粒。

Kuroda Makoto, Halfmann Peter J

Cold Atmospheric Plasma Promotes Anti-Inflammatory and Regenerative Responses in Oral Soft Tissue through Redox-Driven Mitochondrial Regulation.

冷大气等离子体通过氧化还原驱动的线粒体调节促进口腔软组织的抗炎和再生反应。

Nguyen Tien Ngoc Thuy, Kim Byung-Hoon

Trex1 overexpression leads to longer lifespans and fragmented sleep in Drosophila melanogaster.

Trex1 过表达导致果蝇寿命延长和睡眠碎片化。

Kim Jeonghan, Mao Stephanie, Negron Yazmin L Serrano, Kumar Shailesh, Zhang Fan, Xu Hong, Harbison Susan T, Kim Myung K, Chung Jay H

Genetic suppressor of autophagy defects in huntingtin null cells identified using a mutagenesis screen in Dictyostelium discoideum.

利用盘基网柄菌诱变筛选鉴定出亨廷顿蛋白缺失细胞中自噬缺陷的遗传抑制因子。

Cuoco Lauren, Gill Shelbi E, Francois Luc, Souders Sarah, DeMasi Caroline, Moore Jeffrey R, Chain Frédéric J J

Diabetes Induces Accumulation of Carbonylated Proteins in the Rat Retinal Pigment Epithelium Independently of Oxidative Stress.

糖尿病可诱导大鼠视网膜色素上皮细胞中羰基化蛋白的积累,且该过程与氧化应激无关。

Morales-Ramírez Elena, Villeda-González Juan David, Sánchez-Chávez Gustavo, Salceda Rocío

Vitamin B3 suppresses Ras(V12) -driven tumor growth in Drosophila via redox regulation, mitochondrial function, and autophagy activation.

维生素 B3 通过氧化还原调节、线粒体功能和自噬激活来抑制果蝇中 Ras(V12) 驱动的肿瘤生长。

Shen Yingying, Tang Yafang, Fan Xueting, Qin Xiaoqiu, Wang Wenjing, Li Qinghua

Cooperative Architecture of Mitochondrial Proteome Homeostasis.

线粒体蛋白质组稳态的协同结构。

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