Anti-beta Actin antibody [mAbcam 8226] - Loading Control (Ab8226)

推荐稀释比例

• IHC-P0.1-0.5 µg/mL Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.
• WB1 µg/mL We recommend blocking using 2-5% BSA as we have found that use of 5% milk significantly reduces the band intensity for beta actin. Please refer to the images section for the blocking comparison data.
• ICC/IF5 µg/mL

Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab8226) is a mouse monoclonal antibody and is validated for use in ICC/IF, IHC-P and WB. Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab8226) was first used in a scientific publication in 2005 and has been cited over 3363 times in peer reviewed journals. It's performance in Western blot in human, mouse and rat samples is trusted by the scientific community. Abcam's high quality validation processes ensure Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab8226) has high sensitivity and specificity. Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab8226) has 137 independent reviews from customers. beta Actin antibodies are often used as loading controls in Western Blot. Anti-beta Actin antibody [mAbcam 8226] - Loading Control has been verified in Western Blot samples and detects a band at 42kDa Molecular weight. Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab8226) specifically detects beta Actin (UniProt ID: P60709; Molecular weight: 42kDa) and is sold in 100 ug selling sizes. Conjugation-ready, carrier free format available for antibody clone mAbcam 8226 - ab264083. Antibody clone mAbcam 8226 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor ® 68, Alexa Fluor ® 79, HRP ( ab18492, ab184576, ab2272 ). Beta-Actin (β-actin) is a highly conserved protein that plays a crucial role in cell motility, structure and integrity. It is one of the six actin isoforms found in humans and is a major component of the cytoskeleton in non-muscle cells. β-actin is involved in various cellular processes, including cell division, signaling and maintaining cell shape. Due to its ubiquitous expression and stability, β-actin is commonly used as a loading control in western blotting (WB) and other protein quantification assays. Understanding β-actin's functions and interactions is essential for studying cellular dynamics and various physiological processes.