Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody (Ab1162)

DDDDK epitope tag, DDDK, DYKDDDDK, DYKDDDDK (also known as the FLAG搴 tag sequence), DYKDDDDK epitope tag, DYKDDDDK tag, ECS epitope tag, ECS tag, Enterokinase Cleavage Site epitope tag, Enterokinase Cleavage Site tag, FLAG搴, FLAG搴 tag, ddk, flag, flag tag

抗原名称:DDDDK tag (Binds to FLAG® tag sequence)
应用:WB, ELISA, IF/ICC

详细信息

宿主/亚型Rabbit / IgG
抗体类型Polyclonal
产品形式Liquid
纯化方式Affinity purification Immunogen
储存缓冲液pH: 7.2 Preservative: 0.1% Sodium azide Constituents: PBS
储存条件运输条件: Blue Ice 推荐的短期储存时间: 1-2 weeks 推荐的短期储存条件: +4°C 推荐的长期储存条件: -20°C 储存信息: Avoid freeze / thaw cycle
无载体蛋白No

背景信息

Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody (ab1162) is a rabbit polyclonal antibody and is validated for use in ELISA, ICC, WB in human, mouse, rat samples. Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody (ab1162) has been cited over 166 times in peer reviewed journals and is trusted by the scientific community. Abcam's high quality validation processes ensure Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody (ab1162) has high sensitivity and specificity. Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody (ab1162) has 13 independent reviews from customers. DDDDK tag (Binds to FLAG® tag sequence) antibodies are used to visualize proteins labelled with this tag in a variety of applications (for example imaging and Flow cytometry). To enable specific detection of your tagged protein, Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody (ab1162) has been validated in ELISA, ICC, WB. Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody (ab1162) and is sold in 100 µg selling sizes. Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Tagging with xxxDDDDK may be done at the N-terminus, N-terminus preceded by a methionine residue, C-terminus, and in internal positions of the target protein. The small size of the epitope tag and its high hydrophilicity tend to decrease the possibility of interference with protein expression, proteolytic maturation, antigenicity and function. The enterokinase cleavage site allows it to be completely removed from the purified fusion proteins. FLAG® is a registered trade mark of Sigma Aldrich Biotechnology LP. It is used here for informational purposes only.

文献引用 (182)

Cholesterol analogs modulate lipid nanoparticle performance for mRNA delivery after lyophilization and enable ocular disease therapy.

胆固醇类似物可调节脂质纳米颗粒在冻干后递送 mRNA 的性能,从而实现眼部疾病的治疗。

Wang Ting, Li Wanqi, Chen Jianyang, Cheng Sitian, Gao Min, Jiang Chengyu, Jiang Chen, Hu Bingyi, Sung Tzu-Cheng, Higuchi Akon

Palmitoylation Induced Activation of SMAD3 Exacerbates Colitis by Promoting Tissue-resident Memory T Cells Differentiation.

棕榈酰化诱导的 SMAD3 活化通过促进组织驻留记忆 T 细胞分化加剧结肠炎。

Xu Yuejie, Ji Siqi, Jiang Ping, Zhao Zhenguo, Zhou Qian, Su Dan, Ullah Shafi, Zhao Yu, Zou Xiaoping, Zhang Mingming, Sun Jing, Xu Guifang, Tang Jian

Enhanced genome replication activity of pandemic H1N1 influenza A virus through PA mutations.

通过PA突变增强大流行性H1N1甲型流感病毒的基因组复制活性。

Schmierer Jordana, Lutz Michael, Takimoto Toru

Elevated Piezo levels cause structural and functional alterations in Drosophila garland nephrocytes.

Piezo 水平升高会导致果蝇 garland 肾细胞的结构和功能发生改变。

Hazelton-Cavill Paris, Alornyo Karl K, Bouchard Michelle, Schulz Kristina, Huber Tobias B, Denholm Barry, Koehler Sybille

Inhibition of HDAC6 alters fumarate hydratase activity and mitochondrial structure.

抑制 HDAC6 会改变延胡索酸水合酶活性和线粒体结构

Roe Andrew, Dowling Catríona M, D'Arcy Cian, Alencar Rodrigues Daniel, Wang Yu, Hiller Matthew, Keogh Carl, Hollinshead Kate E R, Garre Massimiliano, Cavanagh Brenton, Wynne Kieran, Liu Tianyan, Chen Zhixing, Kerr Emma, McIlroy Marie, H M Prehn Jochen, Schoen Ingmar, Chonghaile Tríona Ní

Lipid nanoparticles for mRNA delivery in brain via systemic administration

通过全身给药将mRNA递送至大脑的脂质纳米颗粒

Dinglingge Cao ,Xucheng Hou ,Chang Wang ,Siyu Wang ,Zhengwei Liu ,Meng Tian ,Kaiyuan Guo ,Haoyuan Li ,Diana D Kang ,Yichen Zhong ,Yonger Xue ,Changyue Yu ,Binbin Deng ,Yizhou Dong

A pH-responsive PEG coating strategy for enhancing the enrichment of small extracellular vesicles towards disease regions with acidic microenvironment.

一种 pH 响应型 PEG 涂层策略,用于增强小细胞外囊泡向具有酸性微环境的疾病区域的富集

Zhao Jianwei, Niu Xinyu, Luo Lei, Yuan Ji, Zhang Juntao, Niu Xin, Tian Hengli, Yang Yunlong, Deng Zhifeng, Wang Yang

Intestinal NUCB2/nesfatin-1 regulates hepatic glucose production via the MC4R-cAMP-GLP-1 pathway

肠道 NUCB2/nesfatin-1 通过 MC4R-cAMP-GLP-1 通路调节肝葡萄糖生成

Shan Geng #, Shan Yang #, Xuejiao Tang, Shiyao Xue, Ke Li, Dongfang Liu, Chen Chen, Zhiming Zhu, Hongting Zheng, Yuanqiang Wang, Gangyi Yang, Ling Li, Mengliu Yang

SMAD2 S-palmitoylation promotes its linker region phosphorylation and T(H)17 cell differentiation in a mouse model of multiple sclerosis.

在多发性硬化症小鼠模型中,SMAD2 S-棕榈酰化促进其连接区磷酸化和 T(H)17 细胞分化

Zhang Mingming, Yu Tao, Liu Yinong, Lu Xuan, Chen Wenzhe, Zhou Lixing, Xu Yuejie, Yang Min, Miller Andrew D, Lin Hening

A Sugarcane Smut Fungus Effector Hijacks Plant Vacuolar Sorting Receptor-Mediated Trafficking to Evade Host Immune Detection.

甘蔗黑粉菌效应蛋白劫持植物液泡分选受体介导的运输以逃避宿主免疫检测

Ling Hui, Fu Xueqin, Huang Ning, Zhong Zaofa, Liu Tingting, Cui Haitao, Que Youxiong
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