Anti-beta Actin antibody - Loading Control (Ab8227)

推荐稀释比例

• IHC-P0.2-1 µg/mL We recommend Goat Anti-Rabbit IgG H&L (Biotin) ( ab6720 ) secondary antibody or Goat Anti-Rabbit IgG Fc (HRP) ( ab97200 ) secondary antibody. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.
• WB1/1000 - 1/5000 We recommend Goat Anti-Rabbit IgG H&L (HRP) ( ab6721 ) secondary antibody. A stronger signal is observed in chemiluminescent western blot when using 2% BSA as the blocking agent. Milk blocking is suitable for use with fluorescent detection systems.
• ICC/IF1 µg/mL

Anti-beta Actin antibody (ab8227) is a rabbit polyclonal antibody and is validated for use in ICC/IF, IHC-P and WB. Anti-beta Actin antibody (ab8227) was first used in a scientific publication in 1991 and has been cited over 4031 times in peer reviewed journals. It's performance in Western blot in human, mouse and rat samples is trusted by the scientific community. Abcam's high quality validation processes ensure Anti-beta Actin antibody (ab8227) has high sensitivity and specificity. Anti-beta Actin antibody (ab8227) has 104 independent reviews from customers. beta Actin antibodies are often used as loading controls in Western Blot. Anti-beta Actin antibody has been verified in Western Blot samples and detects a band at 42kDa Molecular weight. Anti-beta Actin antibody (ab8227) specifically detects beta Actin (UniProt ID: P60709; Molecular weight: 42kDa) and is sold in 50 µg selling sizes. Beta-Actin (β-actin) is a highly conserved protein that plays a crucial role in cell motility, structure and integrity. It is one of the six actin isoforms found in humans and is a major component of the cytoskeleton in non-muscle cells. β-actin is involved in various cellular processes, including cell division, signaling and maintaining cell shape. Due to its ubiquitous expression and stability, β-actin is commonly used as a loading control in western blotting (WB) and other protein quantification assays. Understanding β-actin's functions and interactions is essential for studying cellular dynamics and various physiological processes.