Anti-GAPDH antibody [6C5] - Loading Control (Ab8245)

GAPD, CDABP0047, OK/SW-cl.12, GAPDH, Glyceraldehyde-3-phosphate dehydrogenase, Peptidyl-cysteine S-nitrosylase GAPDH

反应种属:Human, Mouse, Rat
抗原名称:GAPDH
应用:WB, IF/ICC

用户验证的应用

推荐稀释比例

  • WB1/500 - 1/10000
  • ICC/IF1-5 µg/mL

详细信息

免疫原Native Full Length Protein corresponding to Rabbit GAPDH. P46406
宿主/亚型Mouse / IgG1
抗体类型Monoclonal
全称Anti-GAPDH antibody [6C5] - Loading Control
产品形式Liquid
储存缓冲液pH: 7.4 Preservative: 0.09% Sodium azide Constituents: PBS
储存条件运输条件: Blue Ice 推荐的短期储存时间: 1-2 weeks 推荐的短期储存条件: +4°C 推荐的长期储存条件: -20°C 储存信息: Avoid freeze / thaw cycle
纯化方式Affinity purification Protein A

背景信息

Anti-GAPDH antibody [6C5] - Loading Control (ab8245) is a mouse monoclonal antibody and is validated for use in ICC/IF and WB. Anti-GAPDH antibody [6C5] - Loading Control (ab8245) was first used in a scientific publication in 2003 and has been cited over 5105 times in peer reviewed journals. It's performance in Western Blot in human, mouse and rat samples is trusted by the scientific community. Abcam's high quality validation processes ensure Anti-GAPDH antibody [6C5] - Loading Control (ab8245) has high sensitivity and specificity. Anti-GAPDH antibody [6C5] - Loading Control (ab8245) has 100 independent reviews from customers. GAPDH antibodies are often used as loading controls in Western Blot. Anti-GAPDH antibody [6C5] - Loading Control has been verified in Western Blot samples and detects a band at 36kDa Molecular weight. Anti-GAPDH antibody [6C5] - Loading Control (ab8245) specifically detects GAPDH (UniProt ID: P46406; Molecular weight: 36kDa) and is sold in 100 µg selling sizes. One of the top cited antibody in the market for GAPDH with >6500 citations and >70 five star reviews. GAPDH is a key target involved in glycolysis and cell metabolism. It plays a crucial role as a housekeeping gene, particularly in understanding GAPDH expression and its function as a metabolic enzyme. GAPDH is widely analysed in GAPDH activity assays and studies of its role in various cellular processes. Additionally, GAPDH is commonly used as a loading control in Western blot and immunocytochemistry/immunofluorescence (ICC/IF) experiments.

文献引用 (5964)

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肝脏活性素通过血管系统逆转衰老和阿尔茨海默病相关的记忆丧失。

Bieri Gregor, Pratt Karishma J B, Fuseya Yasuhiro, Aghayev Turan, Sucharov Juliana, Horowitz Alana M, Philp Amber R, Fonseca-Valencia Karla, Chu Rebecca, Phan Mason, Remesal Laura, Wang Shih-Hsiu J, Yang Andrew C, Casaletto Kaitlin B, Villeda Saul A

GGC repeat expansions within new open reading frames are translated into toxic polyglycine proteins in oculopharyngodistal myopathy.

在眼咽远端肌病中,新的开放阅读框内的 GGC 重复扩增被翻译成有毒的聚甘氨酸蛋白。

Boivin Manon, Yu Jiaxi, Eura Nobuyuki, Schmitt Léa, Pietri David, Grandgirard Erwan, Goetz-Reiner Patrice, Plassard Damien, Nahy Chadia, Maglott Anne, Morlet Bastien, Gao Chao, Lefebvre Elise, Philipps Muriel, Eberling Pascal, Pichot Angélique, Rossolillo Paola, Thibault Christelle, Oulad-Abdelghani Mustapha, Nishino Ichizo, Yang Kang, Wang Ning, Wang Zhaoxia, Deng Jianwen, Charlet-Berguerand Nicolas

Lithium-Charged Gold Nanoparticles: A New Powerful Tool for Lithium Delivery and Modulation of Glycogen Synthase Kinase 3 Activity.

锂离子金纳米粒子:一种用于锂离子输送和调节糖原合成酶激酶 3 活性的新型强效工具。

Buonerba Antonio, Puliatti Giulia, Puma Domenica Donatella Li, Bandiera Bruno, Cannata Beatrice, Marcocci Maria Elena, Castagno Nicolina, Contento Irene, Impemba Salvatore, Scognamiglio Mariarosa, Girolamo Rocco Di, Naddeo Vincenzo, Canton Patrizia, Capacchione Carmine, Sposito Laura, Albini Martina, Pastore Francesco, Baroni Silvia, Grassi Alfonso, Grassi Claudio, Piacentini Roberto

CEBPB-Regulated Gastric Cell Plasticity Promotes Liver Metastasis of Gastric Cancer.

CEBPB调控的胃细胞可塑性促进胃癌肝转移。

Zuo Zhixiang, Liang Jiaqi, He Li-Na, Xu Kai, Hu Muren, Zhang Kunming, Gao Wei, Yin Junyi, Zhang Lanlin, Ma Boning, Hu Zhiqian, Zhang Pengfei, Jiang Hong

Ectopic expression of cytosolic DHODH uncouples de novo pyrimidine biosynthesis from mitochondrial electron transport.

胞质 DHODH 的异位表达使嘧啶从头合成与线粒体电子传递脱钩。

Curtabbi Andrea, Jaroszewicz Sara Natalia, Sanz-Cortés Rocío, Acín-Pérez Rebeca, Prymidis Dimitrios, Cherevatenko Maksym, Martínez-de-Mena Raquel, Esteban-Amo María Jesús, de la Fuente Miguel A, Frezza Christian, Enríquez José Antonio

DNA damage in macrophages drives immune autoreactivity via nuclear antigen presentation.

巨噬细胞中的DNA损伤通过核抗原呈递驱动免疫自身反应。

RPA exhaustion activates SLFN11 to eliminate cells with heightened replication stress.

RPA 耗竭会激活 SLFN11,从而清除复制压力升高的细胞。

Stanage Tyler H, Li Shudong, Segura-Bayona Sandra, Idilli Aurora I, Millar Rhona, Hewitt Graeme, Boulton Simon J

Piezo1-Targeted Magnetoacoustic Nanobubbles Rescue Alzheimer's Pathology by Electromechanical Neuromodulation and Amyloid-β Clearance.

靶向压电1的磁声纳米气泡通过电机械神经调节和淀粉样蛋白β清除来挽救阿尔茨海默病病理。

Chu Fangxuan, Zhou Xiaoqing, Ma Ren, Liu Ruixu, Liu Xu, Zhu Kai, Wang Yuheng, Wang Xin, Li Ying, Zhang Shunqi, Yin Tao, Liu Zhipeng

A missense variant in ASCL5 leads to lobodontia.

ASCL5基因的错义变异会导致牙叶畸形。

Theerapanon Thanakorn, Intarak Narin, Rattanapornsompong Khanti, Thaweesapphithak Sermporn, Sriwattanapong Kanokwan, Prommanee Sasiprapa, Kulvitit Sirinya, Skrinjaric Tomislav, Samaranayake Lakshman, Pongpanich Monnat, Yeetong Patra, Chaivoravitsakul Nardtiwa, Mehl Nicole Sirisopit, Assawapitaksakul Adjima, Srichomthong Chalurmpon, Chetruengchai Wanna, Porntaveetus Thantrira, Shotelersuk Vorasuk

Injectable hydrogels for osteomyelitis treatment induce metabolic reprogramming for protection against reinfection.

用于治疗骨髓炎的注射用水凝胶可诱导代谢重编程,从而防止再次感染。

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