用户验证的应用
经验证应用
| 项目 | 详情 |
|---|---|
| Positive WB detected in | A549 cells, HeLa cells, mouse skeletal muscle tissue, rat skeletal muscle tissue, HEK-293, HepG2 cells, MCF-7 cells, PC-3 cells, C6 cells, HEK-293 cells, HT-1080 cells, HUVEC cells, C2C12 cells |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human colon cancer tissue, human stomach cancer tissue, human endometrial cancer tissue, mouse colon tissue, rat colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释说明
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. Sample-dependent, Check data in validation data gallery.
详细信息
背景信息
SMAD2, also named as MADH2 and MADR2, belongs to the dwarfin/SMAD family, contains 1 MH1 (MAD homology 1) domain and 1 MH2 (MAD homology 2) domain. SMAD2 is a receptor-regulated SMAD(R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. This protein may act as a tumor suppressor in colorectal carcinoma. It is phosphorylated on one or several of Thr-220, Ser-245, Ser-250, and Ser-255. In response to TGF-beta, It is phosphorylated on Ser-465/467 by TGF-beta and activin type 1 receptor kinases, and then able to interact with SMURF2, recruiting other proteins, such as SNON, for degradation. In response to decorin, the naturally occurring inhibitor of TGF-beta signaling, it is phosphorylated on Ser-240 by CaMK2. It is phosphorylated by MAPK3 upon EGF stimulation; which increases transcriptional activity and stability, and is blocked by calmodulin. In response to TGF-beta, it is ubiquitinated by NEDD4L, which promotes its degradation. In response to TGF-beta signaling, it is acetylated on Lys-19 by coactivators, which increases transcriptional activity. This antibody is a rabbit polyclonal antibody raised against residues near the N terminus of human SMAD2. The molecular weight of unphosphorylated forms of Smad2 is 52 kDa and phosphorylated forms of Smad2 is 58 kDa. (PMID: 9006934). The ubiquitination form of Smad2 is ~70 kDa (PMID: 25998442).
文献引用 (57)
Phosphorylation of FOXN3 by NEK6 promotes pulmonary fibrosis through Smad signaling
NEK6 磷酸化 FOXN3 通过 Smad 信号促进肺纤维化
Single-cell RNA sequencing reveals that myeloid S100A8/A9 is a novel regulator of the transition from adaptive hypertrophy to heart failure after pressure overload.
单细胞 RNA 测序显示，髓系 S100A8/A9 是压力超负荷后从适应性肥大到心力衰竭转变的新型调节因子
Dynamic multi-omics and mechanistic modeling approach uncovers novel mechanisms of kidney fibrosis progression
动态多组学和机制建模方法揭示了肾纤维化进展的新机制
Cancer-associated Fibroblast-like Cells Promote Osteosarcoma Metastasis by Upregulation of Phosphoserine Aminotransferase 1 and Activation of the mTOR/S6K Pathway
癌症相关成纤维细胞样细胞通过上调磷酸丝氨酸氨基转移酶1和激活mTOR/S6K通路促进骨肉瘤转移
Blockade of neddylation through targeted inhibition of DCN1 alleviates renal fibrosis.
通过靶向抑制 DCN1 来阻断 neddylation 可减轻肾纤维化
Lactobacillus rhamnosus GG attenuates MASLD/MASH progression by modulating gut microbiota and metabolic pathways.
鼠李糖乳杆菌 GG 通过调节肠道菌群和代谢途径来减弱 MASLD/MASH 的进展
Multi-omics analyses, cell experiments, and network pharmacology tools identified key proteins and candidate drugs for alopecia areata treatment.
多组学分析、细胞实验和网络药理学工具确定了斑秃治疗的关键蛋白质和候选药物
Activin A promoted the anti-tumor effect of ActRIIA high CD8+ T cells in mouse hepatoma
Activin A促进了ActRIIA高表达CD8+ T细胞在小鼠肝癌中的抗肿瘤作用
Mechanistic insights into Y-Box binding protein-1 mediated regulation of lipid metabolism and oxidative stress in NAFLD via INHBE/TNF-β pathway.
通过 INHBE/TNF-β 通路，Y-Box 结合蛋白-1 介导的 NAFLD 脂质代谢和氧化应激调控的机制研究
N6-methyladenosine-modified circSLCO1B3 promotes intrahepatic cholangiocarcinoma progression via regulating HOXC8 and PD-L1
N6-甲基腺苷修饰的 circSLCO1B3 通过调节 HOXC8 和 PD-L1 促进肝内胆管癌进展
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