(12789-1-AP)
用户验证的应用
经验证应用
| 项目 | 详情 |
|---|---|
| Positive WB detected in | A431 cells, HepG2 cells, Jurkat cells, HEK-293 cells, HL-60 cells, U-251 cells, U-937 cells, MCF-7 cells, U2OS cells |
| Positive IP detected in | HL-60 cells, HepG2 cells, MCF-7 cells |
| Positive IHC detected in | human tonsillitis tissue, human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Jurkat cells |
| Positive FC (Intra) detected in | Jurkat cells, HeLa cells |
推荐稀释比例
- Western Blot (WB)1:2000-1:10000
- Immunoprecipitation (IP)0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
- Immunohistochemistry (IHC)1:2000-1:8000
- Immunofluorescence (IF)/ICC1:50-1:500
- Flow Cytometry (FC) (INTRA)0.25 ug per 10^6 cells in a 100 µl suspension
推荐稀释说明
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. Sample-dependent, Check data in validation data gallery.
详细信息
背景信息
1. What is Bcl-2? Bcl-2 (B-cell lymphoma 2) is an outer mitochondrial membrane protein that via heterodimerization with BAX proteins controls apoptosis. Abnormalities of Bcl-2 activity can lead to cancer, autoimmune diseases, and schizophrenia. 2. FAQs for Bcl-2 a. How to measure Bcl-2 and Bax apoptotic markers by western blotting Generally, Bcl-2 protein is considered to have anti-apoptotic activity, while Bax has apoptotic activity. Comparing the ratio between Bcl-2 and Bax protein levels in different samples or cells subjected to treatments can reflect the apoptotic state of the cells. Expression of Bax can vary between cell lines and it is important to compare it to Bcl-2 levels. b. What band size should I expect in western blotting for Bcl-2? The molecular weight of Bcl-2 is 26 kDa.
文献引用 (679)
Recombinant tissue plasminogen activator protects neurons after intracerebral hemorrhage through activating the PI3K/AKT/mTOR pathway.
重组组织型纤溶酶原激活剂通过激活 PI3K/AKT/mTOR 通路来保护脑出血后的神经元
Kit-mediated autophagy suppression driven by a viral oncoprotein emerges as a crucial survival mechanism in Merkel cell carcinoma.
病毒癌蛋白驱动的 Kit 介导的自噬抑制成为默克尔细胞癌的关键生存机制
In Situ Transformable Nanoparticle Effectively Suppresses Bladder Cancer by Damaging Mitochondria and Blocking Mitochondrial Autophagy Flux.
原位可转化纳米颗粒通过破坏线粒体和阻断线粒体自噬流有效抑制膀胱癌
Icaritin Represses Autophagy to Promote Colorectal Cancer Cell Apoptosis and Sensitized Low-Temperature Photothermal Therapy via Targeting HSP90-TXNDC9 Interactions.
淫羊藿苷通过靶向 HSP90-TXNDC9 相互作用抑制自噬，促进结直肠癌细胞凋亡并增强低温光热疗法的敏感性
Microglia-Derived Interleukin-6 Triggers Astrocyte Apoptosis in the Hippocampus and Mediates Depression-Like Behavior.
小胶质细胞衍生的白细胞介素-6 触发海马体中的星形胶质细胞凋亡，并介导抑郁样行为
Targeting MAD2B as a strategy for ischemic stroke therapy.
以 MAD2B 为靶点治疗缺血性卒中
Integrated stress response activation induced by usnic acid alleviates BCL-2 inhibitor ABT-199 resistance in acute myeloid leukemia.
松萝酸诱导的整合应激反应激活可缓解急性髓系白血病中 BCL-2 抑制剂 ABT-199 的耐药性
Single-cell RNA sequencing reveals anterograde trans-synaptic degeneration and exacerbated synaptic remodeling in myopia.
单细胞 RNA 测序揭示近视中顺行跨突触退化和突触重塑加剧
Stimulating Soluble Guanylyl Cyclase with the Clinical Agonist Riociguat Restrains the Development and Progression of Castration-Resistant Prostate Cancer
使用临床激动剂利奥西呱刺激可溶性鸟苷酸环化酶可抑制去势抵抗性前列腺癌的发生和发展。
Absence of astrocytic ceruloplasmin reverses the senescence process with aging of learning and memory abilities.
星形胶质细胞铜蓝蛋白的缺失会逆转衰老过程中学习和记忆能力的衰退过程
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