Lamin B1 Polyclonal antibody (12987-1-AP)
LMNB1; laminB1; Lamin-B1; LMN2; LMNB
用户验证的应用
经验证应用
| 项目 | 详情 |
|---|---|
| Positive WB detected in | HeLa cells, NIH/3T3 cells, HepG2 cells, Jurkat cells, MCF-7 cells, K-562 cells, RAW 264.7 cells, mouse heart tissue, rat heart tissue |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | mouse heart tissue, human colon tissue, human colon cancer tissue, human hepatocellular carcinoma, mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | HEK-293 cells |
推荐稀释说明
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. Sample-dependent, Check data in validation data gallery.
详细信息
背景信息
Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1. Expression of uncleavable mutant lamin A or B caused significant delays in the onset of chromatin condensation and nuclear shrinkage during apoptosis (PMID:11953316). This protein is not suitable for samples where the nuclear envelope has been removed.
文献引用 (468)
Serine/threonine/tyrosine kinase 1 drives pancreatic carcinogenesis via GSK3β sequestration-mediated Wnt/β-catenin pathway hyperactivation.
丝氨酸/苏氨酸/酪氨酸激酶 1 通过 GSK3β 隔离介导的 Wnt/β-catenin 通路过度激活驱动胰腺癌的发生
Chromosome mis-segregation triggers cell cycle arrest through a mechanosensitive nuclear envelope checkpoint
染色体错误分离通过机械敏感的核膜检查点触发细胞周期停滞
The ZBTB24-CDCA7-HELLS axis suppresses the totipotent 2C-like reprogramming by maintaining Dux methylation and repression
ZBTB24-CDCA7-HELLS轴通过维持Dux甲基化和抑制作用来抑制全能性2C样重编程。
AKAP1/PKA-mediated GRP75 phosphorylation at mitochondria-associated endoplasmic reticulum membranes protects cancer cells against ferroptosis.
AKAP1/PKA介导的GRP75在与线粒体相关的内质网膜上的磷酸化可保护癌细胞免受铁死亡的影响
Adipose progenitor cell-derived extracellular vesicles suppress macrophage M1 program to alleviate midlife obesity
脂肪祖细胞来源的细胞外囊泡抑制巨噬细胞M1程序，从而缓解中年肥胖
MARTRE family proteins negatively regulate CCR4-NOT activity to protect poly(A) tail length and promote translation of maternal mRNA
MARTRE 家族蛋白负向调控 CCR4-NOT 活性以保护 poly(A) 尾长度并促进母体 mRNA 的翻译
8q24 derived ZNF252P promotes tumorigenesis by driving phase separation to activate c-Myc mediated feedback loop
8q24 衍生的 ZNF252P 通过驱动相分离激活 c-Myc 介导的反馈回路来促进肿瘤发生
Phosphorylation of FOXN3 by NEK6 promotes pulmonary fibrosis through Smad signaling
NEK6 磷酸化 FOXN3 通过 Smad 信号促进肺纤维化
Celastrol directly targets LRP1 to inhibit fibroblast-macrophage crosstalk and ameliorates psoriasis progression.
雷公藤内酯醇直接靶向 LRP1，抑制成纤维细胞-巨噬细胞的相互作用，从而改善银屑病的进展
Chemical knockdown of Keap1 and homoPROTAC-ing allergic rhinitis.
Keap1 的化学敲除和 homoPROTAC 引起的过敏性鼻炎
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