PARP1 Polyclonal antibody (13371-1-AP)
DNA ADP-ribosyltransferase PARP1; ARTD1; ADPRT1; ADPRT 1; ADPRT
用户验证的应用
经验证应用
| 项目 | 详情 |
|---|---|
| Positive WB detected in | HeLa cells, Jurkat cells, C6 cells, Fas antibody treated HeLa cells, Cobalt Chloride treated HeLa cells, THP-1 cells |
| Positive IP detected in | K-562 cells |
| Positive IHC detected in | mouse colon tissue, human breast cancer tissue, human lung cancer tissue, mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse testis tissue |
| Positive IF/ICC detected in | HEK-293 cells, MCF-7 cells |
| Positive FC (Intra) detected in | K-562 cells |
推荐稀释说明
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. Sample-dependent, Check data in validation data gallery.
详细信息
背景信息
PARP1 (poly(ADP-ribose) polymerase 1) is a nuclear enzyme catalyzing the poly(ADP-ribosyl)ation of many key proteins in vivo. The normal function of PARP1 is the routine repair of DNA damage. Activated by DNA strand breaks, the PARP1 is cleaved into an 85 to 89-kDa COOH-terminal fragment and a 24-kDa NH2-terminal peptide by caspases during the apoptotic process. The appearance of PARP fragments is commonly considered as an important biomarker of apoptosis. In addition to caspases, other proteases like calpains, cathepsins, granzymes and matrix metalloproteinases (MMPs) have also been reported to cleave PARP1 and gave rise to fragments ranging from 42-89-kDa. This antibody was generated against the C-terminal region of human PARP1 and it recognizes the full-length as well as the cleavage of the PARP1.
文献引用 (184)
Tripartite motif-containing protein 26 promotes colorectal cancer growth by inactivating p53.
含有三方基序的蛋白 26 通过使 p53 失活来促进结直肠癌的生长
PHGDH activation fuels glioblastoma progression and radioresistance via serine synthesis pathway.
PHGDH 激活通过丝氨酸合成途径促进胶质母细胞瘤的进展和放射抗性
Decursin induces FLT3-ITD acute myeloid leukemia cell apoptosis by increasing the expression of the ubiquitin-conjugase UBE2L6.
Decursin 通过增加泛素结合酶 UBE2L6 的表达来诱导 FLT3-ITD 急性髓系白血病细胞凋亡
Bardoxolone displays potent activity against triple negative breast cancer by inhibiting the TRIP13/STAT3 circuit.
Bardoxolone 通过抑制 TRIP13/STAT3 通路，对三阴性乳腺癌表现出强大的活性
USP28 promotes PARP inhibitor resistance by enhancing SOX9-mediated DNA damage repair in ovarian cancer
USP28通过增强卵巢癌中SOX9介导的DNA损伤修复来促进PARP抑制剂耐药性
METTL3 regulates Ambra1 expression in an m6A-YTHDF2-dependent manner to promote mantle cell lymphoma progression.
METTL3 以 m6A-YTHDF2 依赖的方式调节 Ambra1 的表达，从而促进套细胞淋巴瘤的进展
Molecule interacting with CasL-2 enhances tumor progression and alters radiosensitivity in cervical cancer.
与 CasL-2 相互作用的分子可促进肿瘤进展并改变宫颈癌的放射敏感性
Innovative evaluation of selinexor and JQ1 synergy in leukemia therapy via C-MYC inhibition.
通过 C-MYC 抑制对 selinexor 和 JQ1 在白血病治疗中的协同作用进行创新性评估
The deubiquitination-PARylation positive feedback loop of the USP10-PARP1 axis promotes DNA damage repair and affects therapeutic efficacy of PARP1 inhibitor.
USP10-PARP1轴的去泛素化-PARylation正反馈环路促进DNA损伤修复，并影响PARP1抑制剂的治疗效果
Peroxisomal lipid metabolism inhibits Pimozide-induced cancer cell death by regulating ATP homeostasis.
过氧化物酶体脂质代谢通过调节 ATP 稳态抑制匹莫齐特诱导的癌细胞死亡
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