(14395-1-AP)
用户验证的应用
经验证应用
| 项目 | 详情 |
|---|---|
| Positive WB detected in | mouse colon tissue, human placenta tissue, A549 cells, HUVEC cells, rat colon tissue, mouse heart tissue, rat heart tissue, mouse liver tissue, mouse kidney tissue |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | human appendicitis tissue, human colon tissue, human heart tissue, human hysteromyoma tissue, human liver cancer tissue, mouse colon tissue, stromal tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse heart tissue, mouse small intestine tissue, human colon tissue |
| Positive IF-Fro detected in | mouse colon tissue |
| Positive IF/ICC detected in | C2C12 cells, H9C2 cells |
| Positive FC (Intra) detected in | C2C12 cells |
推荐稀释比例
- Western Blot (WB)1:2000-1:12000
- Immunoprecipitation (IP)0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
- Immunohistochemistry (IHC)1:4000-1:16000
- Immunofluorescence (IF)-P1:1000-1:4000
- Immunofluorescence (IF)-FRO1:200-1:800
- Immunofluorescence (IF)/ICC1:800-1:3200
- Flow Cytometry (FC) (INTRA)0.40 ug per 10^6 cells in a 100 µl suspension
推荐稀释说明
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. Sample-dependent, Check data in validation data gallery.
详细信息
背景信息
ACTA2 (also known as α-smooth muscle actin or α-SMA) belongs to the actin family. Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. ACTA2 is primarily expressed in vascular smooth muscle and anti-ACTA2 is commonly used to marker smooth muscle cells. This antibody may cross-react with other isoforms of actins.
文献引用 (465)
Porphyromonas gingivalis aggravates atherosclerotic plaque instability by promoting lipid-laden macrophage necroptosis
牙龈卟啉单胞菌通过促进富含脂质的巨噬细胞坏死,加剧动脉粥样硬化斑块的不稳定性。
Sustained oxygen-releasing hydrogel implants enhance flap regeneration by promoting mitochondrial biogenesis under mild hypoxia.
持续释放氧气的水凝胶植入物通过在轻度缺氧条件下促进线粒体生物合成来增强皮瓣再生
Pathology-inspired collagen-binding thermosensitive micelle drops enable prolonged and efficient treatment of fungal keratitis.
受病理学启发而研制的胶原蛋白结合热敏胶束滴剂能够延长真菌性角膜炎的治疗时间并提高治疗效果
Identifying fibroblast-derived sFRP2 as a therapeutic target and engineering siRNA therapy for uterine scarring.
确定成纤维细胞衍生的 sFRP2 作为治疗靶点,并设计 siRNA 疗法治疗子宫瘢痕
Noncanonical function of Pannexin1 promotes cellular senescence and renal fibrosis post-acute kidney injury.
Pannexin1 的非经典功能促进急性肾损伤后的细胞衰老和肾纤维化
Sintilimab and anlotinib with gemcitabine plus cisplatin in advanced biliary tract cancer: SAGC a randomized phase 2 trial
信迪利单抗和安罗替尼联合吉西他滨和顺铂治疗晚期胆道癌:SAGC 一项随机 II 期试验
Medium from human iPSC-derived primitive macrophages promotes adult cardiomyocyte proliferation and cardiac regeneration
源自人诱导多能干细胞的原始巨噬细胞培养基可促进成体心肌细胞增殖和心脏再生。
Engineering spatially-confined conduits to tune nerve self-organization and allodynic responses via YAP-mediated mechanotransduction
设计空间受限的管道,通过 YAP 介导的机械转导来调节神经自组织和异常性疼痛反应
Inhibited peroxidase activity of peroxiredoxin 1 by palmitic acid exacerbates nonalcoholic steatohepatitis in male mice
棕榈酸抑制过氧化物酶 1 的活性会加重雄性小鼠的非酒精性脂肪性肝炎
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