(14395-1-AP)

反应种属:Human, Mouse, Rat
抗原名称:Alpha smooth muscle actin
应用:WB, IHC, ELISA, FCM, IF/ICC, IF-P, IF-FRO, IP
标记:Unconjugated

用户验证的应用

经验证应用

项目详情
Positive WB detected inmouse colon tissue, human placenta tissue, A549 cells, HUVEC cells, rat colon tissue, mouse heart tissue, rat heart tissue, mouse liver tissue, mouse kidney tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman appendicitis tissue, human colon tissue, human heart tissue, human hysteromyoma tissue, human liver cancer tissue, mouse colon tissue, stromal tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse heart tissue, mouse small intestine tissue, human colon tissue
Positive IF-Fro detected inmouse colon tissue
Positive IF/ICC detected inC2C12 cells, H9C2 cells
Positive FC (Intra) detected inC2C12 cells

推荐稀释比例

  • Western Blot (WB)1:2000-1:12000
  • Immunoprecipitation (IP)0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
  • Immunohistochemistry (IHC)1:4000-1:16000
  • Immunofluorescence (IF)-P1:1000-1:4000
  • Immunofluorescence (IF)-FRO1:200-1:800
  • Immunofluorescence (IF)/ICC1:800-1:3200
  • Flow Cytometry (FC) (INTRA)0.40 ug per 10^6 cells in a 100 µl suspension

推荐稀释说明

It is recommended that this reagent should be titrated in each testing system to obtain optimal results. Sample-dependent, Check data in validation data gallery.

详细信息

免疫原CatNo: Ag5886 Product name: Recombinant human ACTA2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-377 aa of BC017554 Sequence: MCEEEDSTALVCDNGSGLCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHSFYNELRVAPEEHPTLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVTHNVPIYEGYALPHAIMRLDLAGRDLTDYLMKILTERGYSFVTTAEREIVRDIKEKLCYVALDFENEMATAASSSSLEKSYELPDGQVITIGNERFRCPETLFQPSFIGMESAGIHETTYNSIMKCDIDIRKDLYANNVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDEAGPSIVHRKCF
宿主/亚型Rabbit / IgG
抗体类型Polyclonal
产品类型Antibody
全称actin, alpha 2, smooth muscle, aorta
计算分子量377 aa, 42 kDa
实际/观测分子量42 kDa
产品形式Liquid
偶联物Unconjugated
缓冲液配方PBS, Azide, Glycerol
储存缓冲液PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage.
纯化方式Antigen affinity purification
GenBank蛋白编号BC017554
基因名称Alpha smooth muscle actin
Gene ID59
RRIDAB_2223009
UniProt IDP62736

背景信息

ACTA2 (also known as α-smooth muscle actin or α-SMA) belongs to the actin family. Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. ACTA2 is primarily expressed in vascular smooth muscle and anti-ACTA2 is commonly used to marker smooth muscle cells. This antibody may cross-react with other isoforms of actins.

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