(14600-1-AP)
用户验证的应用
经验证应用
| 项目 | 详情 |
|---|---|
| Positive WB detected in | HeLa cells, mouse brain tissue, Chloroquine treated HeLa cells, rat brain tissue |
| Positive IHC detected in | human liver tissue, human gliomas tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Chloroquine treated HeLa cells, Chloroquine treated HepG2 cells |
| Positive FC (Intra) detected in | HeLa cells |
推荐稀释比例
- Western Blot (WB)1:2000-1:8000
- Immunohistochemistry (IHC)1:200-1:1000
- Immunofluorescence (IF)/ICC1:250-1:1000
- Flow Cytometry (FC) (INTRA)0.50 ug per 10^6 cells in a 100 µl suspension
推荐稀释说明
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. Sample-dependent, Check data in validation data gallery.
详细信息
背景信息
Map1LC3, also known as LC3, is the human homolog of yeast Atg8 and is involved in the formation of autophagosomal vacuoles, called autophagosomes. Three human Map1LC3 isoforms, MAP1LC3A, MAP1LC3B, and MAP1LC3C, undergo post-translational modifications during autophagy. And they differ in their post-translation modifications during autophagy. Map1LC3 also exists in two modified forms, an 18 kDa cytoplasmic form that was originally identified as a subunit of the microtubule-associated protein 1, and a 14-16 kDa form that is associated with the autophagosome membrane. This antibody can cross react with MAP1LC3A, MAP1LC3B, and MAP1LC3C.
文献引用 (544)
Bromodomain-containing protein 4 knockdown promotes neuronal ferroptosis in a mouse model of subarachnoid hemorrhage.
在蛛网膜下腔出血的小鼠模型中，溴结构域蛋白 4 的敲低会促进神经元铁死亡
NAP1L1 degradation by FBXW7 reduces the deubiquitination of HDGF-p62 signaling to stimulate autophagy and induce primary cisplatin chemosensitivity in nasopharyngeal carcinoma.
FBXW7 介导的 NAP1L1 降解减少了 HDGF-p62 信号的去泛素化，从而刺激自噬并诱导鼻咽癌对顺铂化疗的敏感性
Biomimetic Nanoparticles Simultaneously Targeting Modulation of Lipid Metabolism and Phenotype of Macrophages for Programmed Atherosclerosis Management.
仿生纳米颗粒同时靶向调节脂质代谢和巨噬细胞表型，用于程序性动脉粥样硬化管理
Comprehensive profiling of lipid metabolic reprogramming expands precision medicine for HCC.
对脂质代谢重编程进行全面分析，可扩大肝细胞癌精准医疗的应用
Programmed enhancement of endogenous iron-mediated lysosomal membrane permeabilization for tumor ferroptosis/pyroptosis dual-induction.
程序性增强内源性铁介导的溶酶体膜通透性，以诱导肿瘤铁死亡/焦亡双重发生
DSS1 inhibits autophagy to activate epithelial-mesenchymal transition in a pro-metastatic niche of renal cell carcinoma.
DSS1抑制自噬，从而激活肾细胞癌促转移微环境中的上皮-间质转化
Redox regulation of TRIM28 facilitates neuronal ferroptosis by promoting SUMOylation and inhibiting OPTN-selective autophagic degradation of ACSL4.
TRIM28 的氧化还原调控通过促进 SUMO 化和抑制 OPTN 选择性自噬降解 ACSL4 来促进神经元铁死亡
TRIM21-driven K63-linked ubiquitination of RBM38c, as a novel interactor of BECN1, contributes to DNA damage-induced autophagy.
TRIM21 驱动的 RBM38c 的 K63 连接的泛素化，作为 BECN1 的新型相互作用蛋白，促进 DNA 损伤诱导的自噬
IGF2BP3-mediated m(6)A modification of RASGRF1 promoting joint injury in rheumatoid arthritis.
IGF2BP3 介导的 RASGRF1 m(6)A 修饰促进类风湿性关节炎的关节损伤
Viruses hijack FPN1 to disrupt iron withholding and suppress host defense
病毒劫持FPN1以干扰铁的保留并抑制宿主防御
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