PCNA Polyclonal antibody (10205-2-AP)
Cyclin; proliferating cell nuclear antigen
用户验证的应用
经验证应用
| 项目 | 详情 |
|---|---|
| Positive WB detected in | HeLa cells, A431 cells, BALB/3T3 clone A31 cells, HEK293 cells, Jurkat cells, MCF-7 cells, PC-12 cells, NIH/3T3 cells, C2C12 cells, mouse spleen tissue, rat spleen tissue |
| Positive IP detected in | MCF-7 cells, HeLa cells |
| Positive IHC detected in | human stomach cancer tissue, human breast cancer tissue, human colon cancer tissue, human liver cancer tissue, human malignant melanoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse testis tissue, human breast cancer tissue |
推荐稀释说明
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. Sample-dependent, Check data in validation data gallery.
详细信息
背景信息
Proliferating Cell Nuclear Antigen, commonly known as PCNA, is a protein that acts as a processivity factor for DNA polymerase δ in eukaryotic cells. This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. PCNA induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. It has to be loaded onto DNA in order to be able to stimulate APEX2. PCNA protein is highly conserved during evolution; the deduced amino acid sequences of rat and human differ by only 4 of 261 amino acids. PCNA has been used as loading control for proliferating cells. This antibody is a rabbit polyclonal antibody raised against an internal region of human PCNA. The calculated molecular weight of PCNA is 29 kDa, but modified PCNA is 36kDa (PMID: 1358458).
文献引用 (335)
CCDC80 Protects against Aortic Dissection and Rupture by Maintaining the Contractile Smooth Muscle Cell Phenotype.
CCDC80 通过维持收缩性平滑肌细胞表型来防止主动脉夹层和破裂
Extracellular matrix stiffness reduces DNA 6Â ma level to facilitate colorectal cancer progression via disrupting P53 binding to CDKN1A promoter.
细胞外基质硬度降低 DNA 6Â ma 水平，通过破坏 P53 与 CDKN1A 启动子的结合来促进结直肠癌的进展
Integrated proteogenomic characterization of ampullary adenocarcinoma.
壶腹腺癌的整合蛋白质基因组学特征分析
Administration of Porphyromonas gingivalis in pregnant mice enhances glycolysis and histone lactylation/ADAM17 leading to cleft palate in offspring
在妊娠小鼠体内注射牙龈卟啉单胞菌可增强糖酵解和组蛋白乳酸化/ADAM17，从而导致子代出现腭裂。
IGF2BP3/ESM1/KLF10/BECN1 positive feedback loop: a novel therapeutic target in ovarian cancer via lipid metabolism reprogramming.
IGF2BP3/ESM1/KLF10/BECN1 正反馈环路：通过脂质代谢重编程治疗卵巢癌的新靶点
FOXP1 phosphorylation antagonizes its O-GlcNAcylation in regulating ATR activation in response to replication stress
FOXP1磷酸化拮抗其O-GlcNAc糖基化，从而在复制应激反应中调节ATR激活。
Whole-gene CRISPR/cas9 library screen revealed targeting STAT6 increased the sensitivity of liver cancer to celecoxib via inhibiting arachidonic acid shunting.
全基因 CRISPR/Cas9 文库筛选显示，靶向 STAT6 可通过抑制花生四烯酸分流来提高肝癌对塞来昔布的敏感性
Deletion of smooth muscle ZFP36 promotes neointimal hyperplasia in mice.
小鼠平滑肌ZFP36基因缺失可促进新生内膜增生
Chromatin assembly factor 1 subunit A promotes TLS pathway by recruiting E3 ubiquitin ligase RAD18 in cancer cells
染色质组装因子 1 亚基 A 通过在癌细胞中募集 E3 泛素连接酶 RAD18 来促进 TLS 通路
LONP1 facilitates pulmonary artery smooth muscle cell glycolytic reprogramming by degrading MPC1 in pulmonary hypertension.
LONP1 通过降解 MPC1 促进肺动脉高压中肺动脉平滑肌细胞的糖酵解重编程
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