Anti-Vimentin antibody [RV202] - Cytoskeleton Marker (Ab8978)

Vimentin, VIM

反应种属:Human, Mouse, Rat, Pig, Fish
抗原名称:VIM
应用:WB, IHC, FCM, IF/ICC

用户验证的应用

推荐稀释比例

  • IHC-P1/100 - 1/1000 We recommend ab92547 as an alternative for IHC-P with mouse samples.
  • ICC/IF1 µg/mL
  • Flow Cyt (Intra)1/100 - 1/200
  • IHC-FrRecommended range is 1/100 - 1/200 for Immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection. For PFA fixed tissue use at 1/1000.

详细信息

免疫原Native Full Length Protein corresponding to Cow VIM. P48616
宿主/亚型Mouse / IgG1
抗体类型Monoclonal
全称Anti-Vimentin antibody [RV202] - Cytoskeleton Marker
产品形式Liquid
储存缓冲液Preservative: 0.09% Sodium azide Constituents: PBS
储存条件运输条件: Blue Ice 推荐的短期储存时间: 1-2 weeks 推荐的短期储存条件: +4°C 推荐的长期储存条件: -20°C 储存信息: Avoid freeze / thaw cycle
纯化方式Affinity purification Protein G

背景信息

Anti-Vimentin antibody [RV202] - Cytoskeleton Marker (ab8978) is a mouse monoclonal antibody and is validated for use in Flow Cyt (Intra), ICC/IF, IHC-Fr, IHC-P, WB in mouse samples. Anti-Vimentin antibody [RV202] - Cytoskeleton Marker (ab8978) has been cited over 570 times in peer reviewed journals and is trusted by the scientific community. Abcam's high quality validation processes ensure Anti-Vimentin antibody [RV202] - Cytoskeleton Marker (ab8978) has high sensitivity and specificity. The specificity of Anti-Vimentin antibody [RV202] - Cytoskeleton Marker (ab8978) has been confirmed by testing in knockout samples. Anti-Vimentin antibody [RV202] - Cytoskeleton Marker (ab8978) has 44 independent reviews from customers. Anti-Vimentin antibody [RV202] - Cytoskeleton Marker (ab8978) specifically detects Vimentin (UniProt ID: P08670; Molecular weight: 54kDa) and is sold in 100 µg selling sizes. Antibody clone RV202 is also available pre-conjugated to FITC for your convenience ( ab128507 ). Vimentin (57 kDa) is the intermediate filament protein (IFP) of mesenchymal cells. This IFP however often deviates from the tissue-specific and developmentally regulated pattern of expression. Besides its typical expression in most cultured cells, vimentin is also expressed together with several other IFPs during early stages of development. As differentiation proceeds, vimentin is exchanged for the tissue-specific intermediate filament type. Also in cancers, vimentin is often expressed in addition to the tissue-specific IFP. RV202 is a mouse monoclonal IgG1 antibody derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with a cytoskeletal vimentin extract of calf lens.

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