mCherry Polyclonal antibody (26765-1-AP)

Red fluorescence protein; red fluorescent protein; RFP; RFP-tag

抗原名称:mCherry
应用:WB, ELISA, FCM, IF/ICC, IP
标记:Unconjugated

用户验证的应用

经验证应用

项目详情
Positive WB detected inTransfected HEK-293 cells, Recombinant protein
Positive IP detected inTransfected HEK-293T cells
Positive IF/ICC detected inTransfected HEK-293 cells
Positive FC (Intra) detected inTransfected HEK-293T cells

推荐稀释说明

It is recommended that this reagent should be titrated in each testing system to obtain optimal results. Sample-dependent, Check data in validation data gallery.

详细信息

免疫原CatNo: Ag25320 Product name: Recombinant mCherry protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-235 aa of Sequence: VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAEGRHSTGGMDELYK
宿主/亚型Rabbit / IgG
抗体类型Polyclonal
产品类型Antibody
产品形式Liquid
偶联物Unconjugated
纯化方式Antigen affinity purification
储存缓冲液PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage.
RRIDAB_2876881

背景信息

Red fluorescent proteins (RFPs) is a collective term referring to a heterogenous group of red chromophore-carrying proteins, originating from various species and forming different protein lineages. The original RFP (dsRed) is a 225 amino acid fluorescent protein (25.9 kDa) derived from Discosoma sp. . It emits red light with a peak wavelength of 593 nm upon excitation by green light (excitation peak at 558 nm). When fused with other proteins, RFP serves as a versatile reporter protein e.g. for quantifying expression levels or facilitates visualization of subcellular localization through fluorescence microscopy. This antibody is a rabbit polyclonal antibody raised against mCherry. It can be used to detect mCherry, dsRed, tdTomato, and mScarlet.

文献引用 (56)

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RNA聚合酶II抑制可激活细胞死亡,而与转录丧失无关。

Nicholas W Harper ,Gavin A Birdsall ,Megan E Honeywell ,Kelly M Ward ,Athma A Pai ,Michael J Lee

Structural basis of SIRT7 nucleosome engagement and substrate specificity

SIRT7 核小体结合和底物特异性的结构基础

Carlos Moreno-Yruela # ,Babatunde E Ekundayo # ,Polina N Foteva ,Dongchun Ni ,Esther Calvino-Sanles ,Henning Stahlberg ,Beat Fierz

Neoadjuvant Chemotherapy With Cisplatin Up-Regulates GSDMD to Enhance Oral Squamous Cell Carcinoma Metastasis Through MMP14-Mediated EMT Activation.

顺铂新辅助化疗通过 MMP14 介导的 EMT 激活上调 GSDMD,从而增强口腔鳞状细胞癌的转移

Huang Zixian, Jiang Qiming, Zhang Qianyu, Lu Nan, Rui Xi, Chen Rui, Wang Yan, Wang Yuepeng, Xu Xiaoding, Huang Zhiquan

Trans-Golgi network tethering factors regulate TBK1 trafficking and promote the STING-IFN-I pathway.

反式高尔基网络连接因子调节 TBK1 运输并促进 STING-IFN-I 通路

Wang Jinrui, Niu Shenghui, Hu Xiao, Li Tianxing, Liu Shengduo, Tu Yingfeng, Shang Zehua, Zhao Lin, Xu Pinglong, Lin Jingwen, Chen Lu, Billadeau Daniel D, Jia Da

AGR2-mediated cell-cell communication controls the antiviral immune response by promoting the thiol oxidation of TRAF3.

AGR2介导的细胞间通讯通过促进TRAF3的硫醇氧化来控制抗病毒免疫反应

Jia Mengqi, Chen Xiaojing, Guo Wenxue, Ma Dapeng, Wang Peng, Niu Huanmin, Liu Changhong, Lin Xianjuan, Lu QiQi, Wang Jing, Zheng Xiaoxue, Sun Qi, Gao Chengjiang, Yuan Huiqing

Monensin suppresses EMT-driven cancer cell motility by inducing Golgi pH-dependent exocytosis of GOLIM4.

莫能菌素通过诱导高尔基体 pH 依赖性 GOLIM4 胞吐作用来抑制 EMT 驱动的癌细胞运动

Tan Xiaochao, Cardin Derrick L, Wang Shike, Xu Yuting, Russell William K

Rbm24a dictates mRNA recruitment for germ granule assembly in zebrafish.

Rbm24a 决定斑马鱼生殖颗粒组装过程中 mRNA 的募集

Zhang Yizhuang, Wang Jiasheng, Fang Hailing, Hu Shuqi, Yang Boya, Zhou Jiayi, Grifone Raphaëlle, Li Panfeng, Lu Tong, Wang Zhengyang, Zhang Chong, Huang Yubin, Wu Dalei, Gong Qianqian, Shi De-Li, Li Ang, Shao Ming

Intranasal administration of mesenchymal stem cells overexpressing FGF21 demonstrates therapeutic potential in experimental Parkinson's disease.

鼻内给药过表达 FGF21 的间充质干细胞在实验性帕金森病中显示出治疗潜力

Lin You-Yen, Chuang De-Maw, Chi Cheng-Yu, Hung Shih-Ya

SARS-CoV-2 NSP3/4 control formation of replication organelle and recruitment of RNA polymerase NSP12.

SARS-CoV-2 NSP3/4 控制复制细胞器的形成和 RNA 聚合酶 NSP12 的募集

Yang Jinping, Tian Buyun, Wang Pei, Chen Rongrong, Xiao Ke, Long Xubing, Zheng Xinyue, Zhu Yun, Sun Fei, Shi Yongxia, Jiu Yaming, Ji Wei, Xue Yanhong, Xu Tao, Li Zonghong

Phase transition of WTAP regulates m(6)A modification of interferon-stimulated genes.

WTAP 的相变调节干扰素刺激基因的 m(6)A 修饰

Cai Sihui, Zhou Jie, Luo Xiaotong, Zhang Chenqiu, Jin Shouheng, Ren Jian, Cui Jun
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