用户验证的应用
经验证应用
| 项目 | 详情 |
|---|---|
| Positive WB detected in | Jurkat cells, RAW 264.7 cells, HeLa cells, HSC-T6 cells, HepG2 cells, ROS1728 cells, NIH/3T3 cells |
| Positive IP detected in | K-562 cells |
| Positive IHC detected in | human lung cancer tissue, mouse colon tissue, mouse testis tissue, human breast cancer tissue, rat colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Neuro-2a cells |
| Positive FC (Intra) detected in | HeLa cells, Jurkat cells |
推荐稀释说明
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. Sample-dependent, Check data in validation data gallery.
详细信息
背景信息
PARP1 (poly(ADP-ribose) polymerase 1) is a nuclear enzyme catalyzing the poly(ADP-ribosyl)ation of many key proteins in vivo. The normal function of PARP1 is the routine repair of DNA damage. Activated by DNA strand breaks, the PARP1 is cleaved into an 85 to 89-kDa COOH-terminal fragment and a 24-kDa NH2-terminal peptide by caspases during the apoptotic process. The appearance of PARP fragments is commonly considered an important biomarker of apoptosis. In addition to caspases, other proteases like calpains, cathepsins, granzymes, and matrix metalloproteinases (MMPs) have also been reported to cleave PARP1 and give rise to fragments ranging from 42-89-kDa. This antibody was generated against the N-terminal region of human PARP1 and it recognizes the full-length as well as the cleavage of the PARP1.
文献引用 (44)
RIPK1 kinase drove brain microvascular endothelial cells death and blood-brain barrier disruption in neonatal Escherichia coli meningitis.
RIPK1 激酶驱动新生儿大肠杆菌脑膜炎中的脑微血管内皮细胞死亡和血脑屏障破坏
Reversing metabolic reprogramming by CPT1 inhibition with etomoxir promotes cardiomyocyte proliferation and heart regeneration via DUSP1 ADP-ribosylation-mediated p38 MAPK phosphorylation
通过 Etomoxir 抑制 CPT1 逆转代谢重编程，通过 DUSP1 ADP 核糖基化介导的 p38 MAPK 磷酸化促进心肌细胞增殖和心脏再生
A new NCL-targeting aptamer-drug conjugate as a promising therapy against esophageal cancer
一种新的靶向 NCL 的适体-药物复合物有望成为治疗食管癌的有希望的疗法
Mutant p53 upregulates HDAC6 to resist ER stress and facilitates Ku70 deacetylation, which prevents its degradation and mitigates DNA damage in colon cancer cells.
突变型 p53 上调 HDAC6 以抵抗内质网应激，并促进 Ku70 去乙酰化，从而防止其降解并减轻结肠癌细胞中的 DNA 损伤
An anoikis-related signature predicts prognosis and immunotherapy response in gastrointestinal cancers.
细胞凋亡相关特征可预测胃肠道癌症的预后和免疫治疗反应
NLRP7 maintains the genomic stability during early human embryogenesis via mediating alternative splicing
NLRP7通过介导选择性剪接来维持早期人类胚胎发育过程中的基因组稳定性。
Enteroviral 3C protease cleaves N4BP1 to impair the host inflammatory response
肠道病毒 3C 蛋白酶裂解 N4BP1 以损害宿主的炎症反应
PGAM1: a potential therapeutic target mediating Wnt/β-catenin signaling drives breast cancer progression
PGAM1：一种潜在的治疗靶点，它介导Wnt/β-catenin信号通路驱动乳腺癌进展
Nuclear IMPDH2 controls the DNA damage response by modulating PARP1 activity
核内IMPDH2通过调节PARP1活性来控制DNA损伤反应。
Enhanced mapping of small-molecule binding sites in cells
增强细胞内小分子结合位点的映射
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