Anti-6X His tag® antibody (Ab9108)

特异性

ELISA: Antibody specificity was verified by ELISA against peptide conjugated to BSA (HHHHHH-BSA). A 1:25,000 dilution of the antibody gave an OD=1.0 in a 15 minute reaction using HRP-conjugated Goat anti-Rabbit IgG at 1:20,000 and TMB as the substrate. Appropriate specificity controls were run.

Anti-6X His tag® antibody (ab9108) is a rabbit polyclonal antibody and is validated for use in ChIP, ELISA, ICC/IF, WB in human, mouse, rat samples. Anti-6X His tag® antibody (ab9108) has been cited over 165 times in peer reviewed journals and is trusted by the scientific community. Abcam's high quality validation processes ensure Anti-6X His tag® antibody (ab9108) has high sensitivity and specificity. Anti-6X His tag® antibody (ab9108) has 25 independent reviews from customers. 6X His tag® antibodies are used to visualize proteins labelled with this tag in a variety of applications (for example imaging and Flow cytometry). To enable specific detection of your tagged protein, Anti-6X His tag® antibody (ab9108) has been validated in ChIP, ELISA, ICC/IF, WB 25 165 Anti-6X His tag® antibody (ab9108) specifically detects 6X His tag® (UniProt ID: P26367; Molecular weight: 47kDa) and is sold in 100 µg selling sizes. This antibody recognizes both C-terminal and N-terminal tags. Abcam recommended secondaries - Goat Anti-Rabbit HRP ( ab205718 ) and Goat Anti-Rabbit Alexa Fluor® 488 ( ab150077 ). Or search our wide range of secondary antibodies for use with your experiment. HIS-TAG® is a trademark of EMD Biosciences, Inc.