mTOR Monoclonal antibody (66888-1-Ig)
FRAP1; FRAP2; FRAP; FKBP12-rapamycin complex-associated protein; EC:2.7.11.1
用户验证的应用
经验证应用
| 项目 | 详情 |
|---|---|
| Positive WB detected in | LNCaP cells, HEK-293 cells, HeLa cells, mouse brain tissue, rat brain tissue, ROS1728 cells, Caco-2 cells, HepG2 cells, Jurkat cells, NCI-H1299 cells, THP-1 cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human colon cancer tissue, human breast cancer tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells, HeLa cells |
推荐稀释说明
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. Sample-dependent, Check data in validation data gallery.
详细信息
背景信息
MTOR, also named as FRAP1, FRAP, FRAP2 and RAPT1, belongs to the PI3/PI4-kinase family. MTOR is a Ser/Thr protein kinase that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth. MTOR is kinase subunit of both mTORC1 and mTORC2, which regulate cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. mTORC2 is also activated by growth factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. MTOR has a calculated molecular mass of 289 kDa, and always can be detected at about 250 kDa due to some modifications (PMID: 14578359).
文献引用 (159)
USP19 potentiates autophagic cell death via inhibiting mTOR pathway through deubiquitinating NEK9 in pancreatic cancer.
USP19 通过去泛素化 NEK9 抑制 mTOR 通路，从而增强胰腺癌细胞的自噬性细胞死亡
KCTD20 suppression mitigates excitotoxicity in tauopathy patient organoids
KCTD20抑制可减轻tau蛋白病患者类器官中的兴奋性毒性
Inhibition of Aberrant Activated Fibroblast-Like Synoviocytes in Rheumatoid Arthritis by Leishmania Peptide via the Regulation of Fatty Acid Synthesis Metabolism.
利什曼原虫肽通过调节脂肪酸合成代谢抑制类风湿性关节炎中异常活化的成纤维细胞样滑膜细胞
Targeting PSMD14 combined with arachidonic acid induces synthetic lethality via FADS1 m6A modification in triple-negative breast cancer
靶向PSMD14联合花生四烯酸可通过FADS1 m6A修饰诱导三阴性乳腺癌的合成致死效应
Carbon dot-based treatment for bacterial pneumonia by promoting a PI3K-mediated M1 polarization of macrophages
通过促进巨噬细胞PI3K介导的M1极化，利用碳点治疗细菌性肺炎
TREM2 affects DAM-like cell transformation in the acute phase of TBI in mice by regulating microglial glycolysis
TREM2通过调节小胶质细胞糖酵解影响小鼠TBI急性期DAM样细胞转化
Long-term leucine supplementation increases body weight in goats by controlling appetite and muscle protein synthesis under protein-restricted conditions.
长期补充亮氨酸可通过控制蛋白质限制条件下的食欲和肌肉蛋白质合成来增加山羊的体重
Alpha-synuclein Fibrils Inhibit Activation of the BDNF/ERK Signaling Loop in the mPFC to Induce Parkinson's Disease-like Alterations with Depression.
α-突触核蛋白原纤维抑制mPFC中BDNF/ERK信号环的激活，从而诱发帕金森病样改变并伴有抑郁症
Hsa_circ_0001304 promotes vascular neointimal hyperplasia accompanied by autophagy activation.
Hsa_circ_0001304 促进血管内膜新生增生，并伴有自噬激活
- 1
- 2
- 3
- 4
- 5
- 6
- 16