用户验证的应用
经验证应用
| 项目 | 详情 |
|---|---|
| Positive WB detected in | HEK-293T cells, HeLa cells, Calyculin A treated NIH/3T3 cells, PC-3 cells, Calyculin A treated HeLa cells, Calyculin A treated PC-3 cells |
| Positive FC (Intra) detected in | Calyculin A treated HepG2 cells |
推荐稀释比例
- Western Blot (WB)1:2000-1:10000
- Flow Cytometry (FC) (INTRA)0.06 ug per 10^6 cells in a 100 µl suspension
推荐稀释说明
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. Sample-dependent, Check data in validation data gallery.
详细信息
背景信息
Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. MEK1 and MEK2 activate p44 and p42 through phosphorylation of activation loop residues Thr202/Tyr204 and Thr185/Tyr187, respectively. Several downstream targets of p44/42 have been identified, including p90RSK and the transcription factor Elk-1. This antibody simultaneously recognizes the phosphorylation of ERK1 at Thr202 and Tyr204, as well as the phosphorylation of ERK2 at Thr185 and Tyr187.
文献引用 (21)
Chimeric antigen receptor with novel intracellular modules improves antitumor performance of T cells
具有新型胞内模块的嵌合抗原受体可提高T细胞的抗肿瘤活性
Integrated stress response activation induced by usnic acid alleviates BCL-2 inhibitor ABT-199 resistance in acute myeloid leukemia.
松萝酸诱导的整合应激反应激活可缓解急性髓系白血病中 BCL-2 抑制剂 ABT-199 的耐药性
Vitamin K-dependent gamma-carboxyglutamic acid protein 1 promotes pancreatic ductal adenocarcinoma progression through stabilizing oncoprotein KRAS and tyrosine kinase receptor EGFR
维生素 K 依赖性 γ-羧基谷氨酸蛋白 1 通过稳定癌蛋白 KRAS 和酪氨酸激酶受体 EGFR 促进胰腺导管腺癌进展
IL-11/IL-11R signal inhibition by 9MW3811 remodels immune tumor microenvironment and enhances anti-tumor efficacy of PD-1 blockade
9MW3811通过抑制IL-11/IL-11R信号通路重塑肿瘤免疫微环境，并增强PD-1阻断的抗肿瘤疗效。
Copper-mediated SEC14L3 promotes cuproptosis to inhibit hepatocellular carcinoma growth via ERK/YY1/FDX1 axis.
铜介导的 SEC14L3 通过 ERK/YY1/FDX1 轴促进铜凋亡，从而抑制肝细胞癌的生长
LncRNA NEAT1 participates in diminished ovarian reserve by affecting granulosa cell apoptosis and estradiol synthesis via the miR-204-5p/ESR1 axis.
LncRNA NEAT1 通过 miR-204-5p/ESR1 轴影响颗粒细胞凋亡和雌二醇合成，从而参与卵巢储备功能下降
Co-administration of isoprenaline and phenylephrine induced a new HFrEF mouse model through activation of both SNS and RAAS.
异丙肾上腺素和苯肾上腺素的共同给药通过激活交感神经系统和肾素-血管紧张素-醛固酮系统，诱导了一种新的HFrEF小鼠模型
A pan-KRAS degrader for the treatment of KRAS-mutant cancers
一种用于治疗KRAS突变型癌症的泛KRAS降解剂
Spinster homolog 2/S1P signaling ameliorates macrophage inflammatory response to bacterial infections by balancing PGE2 production
Spinster同源物2/S1P信号通路通过平衡PGE2的产生来减轻巨噬细胞对细菌感染的炎症反应。
NQO1 promotes osteogenesis and suppresses angiogenesis in DPSCs via MAPK pathway modulation
NQO1 通过调节 MAPK 通路促进 DPSC 中的成骨作用并抑制血管生成
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