用户验证的应用
经验证应用
| 项目 | 详情 |
|---|---|
| Positive WB detected in | HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, rat brain, mouse brain |
| Positive IHC detected in | human skin cancer tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse testis tissue |
| Positive IF-Fro detected in | mouse testis tissue |
| Positive IF/ICC detected in | C2C12 cells, HeLa cells |
推荐稀释比例
- Western Blot (WB)1:5000-1:50000
- Immunohistochemistry (IHC)1:1000-1:4000
- Immunofluorescence (IF)-P1:200-1:800
- Immunofluorescence (IF)-FRO1:200-1:800
- Immunofluorescence (IF)/ICC1:500-1:2000
推荐稀释说明
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. Sample-dependent, Check data in validation data gallery.
详细信息
背景信息
There are five tubulins in human cells: alpha, beta, gamma, delta, and epsilon. Tubulins are conserved across species. They form heterodimers, which multimerize to form a microtubule filament. An alpha and beta tubulin heterodimer is the basic structural unit of microtubules. The heterodimer does not come apart once formed. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha tubulin is useful for scientists across fields as an internal control due to its high, ubiquitous expression pattern.
文献引用 (8)
Tumor-intrinsic TTLL12 drives resistance to cancer immunotherapy via modulating myeloid-derived suppressor cells
肿瘤内在的TTLL12通过调节髓系来源的抑制细胞驱动对癌症免疫疗法的耐药性。
Caspase-9 activates β-catenin signaling to promote pulmonary fibrosis.
Caspase-9 激活 β-catenin 信号通路，促进肺纤维化
Spatial proteomics of ER tubules reveals CLMN, an ER-actin tether at focal adhesions that promotes cell migration
内质网小管的空间蛋白质组学分析揭示了CLMN的存在，CLMN是一种位于黏着斑的内质网-肌动蛋白连接蛋白，可促进细胞迁移。
CircITSN1/EIF4A3/Itsn1 axis mediates postoperative cognitive dysfunction in aged mice: A novel mechanism and therapeutic target.
CircITSN1/EIF4A3/Itsn1 轴介导老年小鼠术后认知功能障碍：一种新的机制和治疗靶点
P300-dependent acetylation of the FOXQ1 complex activates super-enhancers to promote colorectal cancer proliferation and metastasis.
P300 依赖的 FOXQ1 复合物乙酰化激活超级增强子，促进结直肠癌增殖和转移
Charged multivesicular body protein 4C promotes the progression of cervical cancer through the HPV E6/miRâ543 axis.
带电多囊泡体蛋白 4C 通过 HPV E6/miR'543 轴促进宫颈癌的进展
Liraglutide attenuates type 2 diabetes mellitus-associated non-alcoholic fatty liver disease by activating AMPK/ACC signaling and inhibiting ferroptosis
利拉鲁肽通过激活 AMPK/ACC 信号和抑制铁死亡来减轻 2 型糖尿病相关的非酒精性脂肪肝疾病