用户验证的应用
经验证应用
| 项目 | 详情 |
|---|---|
| Positive WB detected in | mouse brain tissue, HEK-293 cells, HeLa cells, Chloroquine treated HEK-293 cells, Chloroquine treated HeLa cells |
| Positive IHC detected in | human colon cancer tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Chloroquine treated HeLa cells |
推荐稀释比例
- Western Blot (WB)1:2000-1:10000
- Immunohistochemistry (IHC)1:250-1:1000
- Immunofluorescence (IF)/ICC1:500-1:2000
推荐稀释说明
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. Sample-dependent, Check data in validation data gallery.
详细信息
背景信息
Map1LC3, also known as LC3, is the human homolog of yeast Atg8 and is involved in the formation of autophagosomal vacuoles, called autophagosomes. Three human Map1LC3 isoforms, MAP1LC3A, MAP1LC3B, and MAP1LC3C, undergo post-translational modifications during autophagy. And they differ in their post-translation modifications during autophagy. Map1LC3 also exists in two modified forms, an 18 kDa cytoplasmic form that was originally identified as a subunit of the microtubule-associated protein 1, and a 14-16 kDa form that is associated with the autophagosome membrane. The antibody 81004-1-RR specifically recognizes LC3B.
文献引用 (17)
Organelle-tuning condition robustly fabricates energetic mitochondria for cartilage regeneration
细胞器调控条件能够有效促进线粒体能量的生成，从而促进软骨再生。
Quercetin alleviates LPS/iE-DAP-induced liver injury by suppressing ferroptosis via regulating ferritinophagy and intracellular iron efflux
槲皮素通过调节铁蛋白自噬和细胞内铁外流抑制铁死亡，从而减轻LPS/iE-DAP诱导的肝损伤。
Chronic sleep deprivation induces plasma exosome-derived miR-150-5p downregulation as a novel mechanism involved in Parkinson's disease progression by targeting DCLK1.
慢性睡眠剥夺通过靶向 DCLK1 诱导血浆外泌体衍生的 miR-150-5p 下调，成为帕金森病进展的一种新机制
NUCKS1 promotes invasion and metastasis of colorectal cancer by stabilizing HDAC2 and activating AKT.
NUCKS1 通过稳定 HDAC2 和激活 AKT 来促进结直肠癌的侵袭和转移
FlaA N/C attenuates radiation-induced lung injury by promoting NAIP/NLRC4/ASC inflammasome autophagy and inhibiting pyroptosis
FlaA N/C 通过促进 NAIP/NLRC4/ASC 炎症小体自噬和抑制细胞焦亡来减轻放射性肺损伤
Runchangningshen paste activates NLRP6 inflammasome-mediated autophagy to stimulate colonic mucin-2 secretion and modulates mucosal microbiota in functional constipation.
润昌宁神膏激活 NLRP6 炎症小体介导的自噬，刺激结肠粘蛋白-2 分泌，调节功能性便秘中的粘膜微生物群
Potential Role of SESN3 in Linking Heart Failure with Preserved Ejection Fraction and Chronic Obstructive Pulmonary Disease via Autophagy Dysregulation.
SESN3 通过自噬失调在射血分数保留型心力衰竭和慢性阻塞性肺病中的潜在作用
The Anti-Inflammatory Effects of Formononetin, an Active Constituent of Pueraria montana Var. Lobata, via Modulation of Macrophage Autophagy and Polarization
葛根素（葛根的活性成分）通过调节巨噬细胞自噬和极化发挥抗炎作用
TREM2 Alleviates Neuroinflammation by Maintaining Cellular Metabolic Homeostasis and Mitophagy Activity During Early Inflammation
TREM2通过在炎症早期维持细胞代谢稳态和线粒体自噬活性来缓解神经炎症
FTO Alleviates Hepatic Ischemia-Reperfusion Injury by Regulating Apoptosis and Autophagy
FTO通过调节细胞凋亡和自噬减轻肝脏缺血再灌注损伤
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