用户验证的应用
经验证应用
| 项目 | 详情 |
|---|---|
| Positive WB detected in | HeLa cells, HEK-293 cells, MCF-7 cells, MOLT-4 cells, Jurkat cells, Raji cells, NIH/3T3 cells, HSC-T6 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC-Autostainer detected in | human colon tissue |
| Positive IF/ICC detected in | HepG2 cells, TNF alpha treated HT-1080 cells |
| Positive FC (Intra) detected in | HepG2 cells |
| Positive ChIP-qPCR detected in | hTNF (30 ng/ml, 1 h) HeLa cells |
推荐稀释比例
- Western Blot (WB)1:5000-1:40000
- Immunoprecipitation (IP)0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
- Immunohistochemistry (IHC)-AUTOSTAINER1:500-1:2000
- Immunofluorescence (IF)/ICC1:125-1:500
- Flow Cytometry (FC) (INTRA)0.40 ug per 10^6 cells in a 100 µl suspension
- CHIP-QPCR1:10-1:100
推荐稀释说明
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. Sample-dependent, Check data in validation data gallery.
详细信息
背景信息
Nuclear factor kB (NF-kB) is a sequence-specific DNA-binding protein complex which regulates the expression of viral genomes, including the human immunodeficiency virus, and a variety of cellular genes, particularly those involved in immune and inflammatory responses. The members of the NF-kB family in mammalian cells include the proto-oncogene c-Rel,p50/p105 (NFkB1), p65 (RelA), p52/p100 (NFkB2), and RelB. All of these proteins share a conserved 300-amino acid region known as the Rel homology domain which is responsible for DNA binding, dimerization, and nuclear translocation of NF-kB. The p65 subunit is a major component of NF-kB complexes and is responsible for trans-activation. NF-kB heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kB p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of IkB upon NF-kB the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kB complex. It associates with chromatin at the NF-kB promoter region via association with DDX1. This antibody is a rabbit monoclonal antibody raised against residues near the N terminus of human RELA.
文献引用 (42)
Chimeric antigen receptor with novel intracellular modules improves antitumor performance of T cells
具有新型胞内模块的嵌合抗原受体可提高T细胞的抗肿瘤活性
Carnosic Acid Directly Targets STING C-Terminal Tail to Improve STING-Mediated Inflammatory Diseases.
鼠尾草酸直接靶向 STING C 端尾部，改善 STING 介导的炎症性疾病
Nanodelivery of Y-27632 by RGD-modified liposome enhances radioimmunotherapy of hepatocellular carcinoma via tumor microenvironment matrix stiffness reprogramming.
通过RGD修饰的脂质体纳米递送Y-27632，通过肿瘤微环境基质硬度重编程增强肝细胞癌的放射免疫疗法
Eupalinolide B inhibits periodontitis development by targeting ubiquitin conjugating enzyme UBE2D3.
Eupalinolide B 通过靶向泛素结合酶 UBE2D3 来抑制牙周炎的发展
ROS-regulated SUR1-TRPM4 drives persistent activation of NLRP3 inflammasome in microglia after whole-brain radiation
全脑辐射后，ROS调控的SUR1-TRPM4驱动小胶质细胞中NLRP3炎症小体的持续激活
Exploring the Anti-PANoptosis Mechanism of Dachaihu Decoction Against Sepsis-Induced Acute Lung Injury: Network Pharmacology, Bioinformatics, and Experimental Validation.
探索大柴胡汤抗脓毒症诱发急性肺损伤的抗PANoptosis机制：网络药理学、生物信息学和实验验证
Modulation of gut microbiota by Gardeniae Fructus oil exerts TLR4/NF-κB/NLRP3 pathway-mediated antidepressant effects based on transcriptomics and fecal transplantation.
基于转录组学和粪便移植，栀子果油通过调节肠道菌群发挥 TLR4/NF-κB/NLRP3 通路介导的抗抑郁作用
Monoclonal Antibodies to Thyrotropin Receptor With Thyroid-Stimulating Activity Activate the NF-κB Pathway to Induce Chemokine Expression.
具有促甲状腺激素活性的抗促甲状腺激素受体单克隆抗体激活 NF-κB 通路诱导趋化因子表达
Cannabidiol Alleviates Intestinal Fibrosis in Mice with Ulcerative Colitis by Regulating Transforming Growth Factor Signaling Pathway
大麻二酚通过调节转化生长因子信号通路减轻溃疡性结肠炎小鼠的肠道纤维化
Association of alterations in transcriptomics and intestinal immune responses with Bifidobacterium longum BAA2573 in improving dextran sulfate sodium-induced colitis.
转录组学和肠道免疫反应的改变与长双歧杆菌 BAA2573 改善葡聚糖硫酸钠诱导的结肠炎之间的关联
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