用户验证的应用
经验证应用
| 项目 | 详情 |
|---|---|
| Positive WB detected in | U-251 cells, rat brain tissue, mouse brain tissue, pig brain tissue |
| Positive IHC-Autostainer detected in | human brain tissue |
| Positive IHC detected in | rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | rat brain tissue, mouse brain tissue |
| Positive IF-Fro detected in | mouse brain tissue, rat brain tissue |
| Positive IF/ICC detected in | U-251 cells |
详细信息
免疫原CatNo: Ag10423
Product name: Recombinant human GFAP protein
Source: e coli.-derived, PGEX-4T
Tag: GST
Domain: 83-432 aa of BC013596
Sequence: YIEKVRFLEQQNKALAAELNQLRAKEPTKLADVYQAELRELRLRLDQLTANSARLEVERDNLAQDLATVRQKLQDETNLRLEAENNLAAYRQEADEATLARLDLERKIESLEEEIRFLRKIHEEEVRELQEQLARQQVHVELDVAKPDLTAALKEIRTQYEAMASSNMHEAEEWYRSKFADLTDAAARNAELLRQAKHEANDYRRQLQSLTCDLESLRGTNESLERQMREQEERHVREAASYQEALARLEEEGQSLKDEMARHLQEYQDLLNVKLALDIEIATYRKLLEGEENRITIPVQTFSNLQIRETSLDTKSVSEGHLKRNIVVKTVEMRDGEVIKESKQEHKDVM
宿主/亚型Rabbit / IgG
抗体类型Recombinant
产品类型Antibody
产品形式Liquid
偶联物Unconjugated
纯化方式Protein A purification
储存缓冲液PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage.
RRIDAB_2923699
UniProt IDP14136
Gene ID2670
背景信息
GFAP (Glial fibrillary acidic protein ), an intermediate-filament (IF) protein , is specifically expressed in cells of astroglial lineage and is widely used to mark astroglia in the brain. It is also used as a marker for intracranial and intraspinal tumors arising from astrocytes.