(81115-1-RR)
用户验证的应用
经验证应用
| 项目 | 详情 |
|---|---|
| Positive WB detected in | HeLa cells, HEK-293 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, rat brain tissue, mouse brain tissue, pig brain tissue |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | rat colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U2OS cells, MDCK cells |
推荐稀释比例
- Western Blot (WB)1:5000-1:50000
- Immunoprecipitation (IP)0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
- Immunohistochemistry (IHC)1:1000-1:4000
- Immunofluorescence (IF)/ICC1:950-1:3800
推荐稀释说明
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. Sample-dependent, Check data in validation data gallery.
详细信息
背景信息
Beta Actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 37-40, 31, 15 kDa cleaved fragment of beta actin can be generated during apoptosis process. This antibody was generated against N-terminal region of human beta actin protein and can cross-react with other actins. (9173887, 11217076, 10229193 )
文献引用 (439)
Fibrotic scar formation after cerebral ischemic stroke: Targeting the Sonic hedgehog signaling pathway for scar reduction.
脑缺血性中风后纤维化瘢痕形成：靶向 Sonic hedgehog 信号通路以减少瘢痕
Porcine decellularized nerve matrix hydrogel attenuates neuroinflammation after peripheral nerve injury by inhibiting the TLR4/MyD88/NF-κB axis
猪源脱细胞神经基质水凝胶通过抑制TLR4/MyD88/NF-κB轴减轻周围神经损伤后的神经炎症。
Nonenzymatic lysine D-lactylation induced by glyoxalase II substrate SLG dampens inflammatory immune responses
由乙二醛酶 II 底物 SLG 诱导的非酶促赖氨酸 D-乳酰化可抑制炎症免疫反应
ZMYND8 drives HER2 antibody resistance in breast cancer via lipid control of IL-27.
ZMYND8 通过脂质控制 IL-27 驱动乳腺癌中的 HER2 抗体耐药性
Chaperone-mediated autophagy directs a dual mechanism to balance premature senescence and senolysis to prevent intervertebral disc degeneration.
分子伴侣介导的自噬指导双重机制，以平衡过早衰老和衰老细胞清除，从而防止椎间盘退变
Harnessing artificial intelligence to identify Bufalin as a molecular glue degrader of estrogen receptor alpha
利用人工智能技术识别布法林为雌激素受体α的分子胶降解剂
Injectable ECM-mimetic dynamic hydrogels abolish ferroptosis-induced post-discectomy herniation through delivering nucleus pulposus progenitor cell-derived exosomes
可注射的 ECM 模拟动态水凝胶通过递送髓核祖细胞衍生的外泌体来消除铁死亡诱导的椎间盘切除术后疝。
Targeting ceramide transfer protein sensitizes AML to FLT3 inhibitors via a GRP78-ATF6-CHOP axis
通过 GRP78-ATF6-CHOP 轴靶向神经酰胺转移蛋白增强 AML 对 FLT3 抑制剂的敏感性
Accumulation of Damaging Lipids in the Arf1-Ablated Neurons Promotes Neurodegeneration through Releasing mtDNA and Activating Inflammatory Pathways in Microglia.
Arf1 缺失神经元中有害脂质的积累通过释放 mtDNA 和激活小胶质细胞中的炎症通路促进神经退行性变
UGDH Lactylation Aggravates Osteoarthritis by Suppressing Glycosaminoglycan Synthesis and Orchestrating Nucleocytoplasmic Transport to Activate MAPK Signaling.
UGDH 乳酸化通过抑制糖胺聚糖合成和协调核质运输激活 MAPK 信号传导来加剧骨关节炎
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