用户验证的应用
经验证应用
| 项目 | 详情 |
|---|---|
| Positive WB detected in | Transfected HEK-293 cells |
| Positive IP detected in | Transfected HEK-293T cells |
| Positive IF/ICC detected in | Transfected HEK-293 cells |
| Positive FC (Intra) detected in | Transfected HEK-293 cells |
推荐稀释比例
- Western Blot (WB)1:5000-1:50000
- Immunoprecipitation (IP)0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
- Immunofluorescence (IF)/ICC1:200-1:800
- Flow Cytometry (FC) (INTRA)0.25 ug per 10^6 cells in a 100 µl suspension
推荐稀释说明
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. Sample-dependent, Check data in validation data gallery.
详细信息
背景信息
Protein tags are a protein or peptide sequences located either on the C- or N- terminal of the target protein, which can facilitate solubility, detection, purification, localization, and expression of the target protein. The HA tag corresponds to amino acid residues YPYDVPDYA of a surface glycoprotein -human influenza virus hemagglutinin (HA). The HA tag is commonly used for a variety of research applications including chromatin immunoprecipitation, ELISA, flow cytometry, western blotting, immunocytochemistry/immunofluorescence among of others.
文献引用 (39)
DNA/RNA-binding protein KIN17 supports esophageal cancer progression via resolving noncanonical STING activation induced by R-loop
DNA/RNA结合蛋白KIN17通过解除R环诱导的非经典STING激活来促进食管癌的进展。
USP19 potentiates autophagic cell death via inhibiting mTOR pathway through deubiquitinating NEK9 in pancreatic cancer.
USP19 通过去泛素化 NEK9 抑制 mTOR 通路，从而增强胰腺癌细胞的自噬性细胞死亡
Deubiquitinase USP13 alleviates doxorubicin-induced cardiotoxicity through promoting the autophagy-mediated degradation of STING.
去泛素化酶 USP13 通过促进自噬介导的 STING 降解来减轻阿霉素引起的心脏毒性
Loss of MNX1 Sensitizes Tumors to Cytotoxic T Cells by Degradation of PD-L1 mRNA.
MNX1 的缺失通过降解 PD-L1 mRNA 使肿瘤对细胞毒性 T 细胞更加敏感
Identification of a Novel Substrate for eEF2K and the AURKA-SOX8 as the Related Pathway in TNBC.
在三阴性乳腺癌中鉴定出 eEF2K 的新底物和 AURKA-SOX8 的相关通路
PPY-Induced iCAFs Cultivate an Immunosuppressive Microenvironment in Pancreatic Cancer.
PPY诱导的iCAFs在胰腺癌中培养免疫抑制微环境
Alternative splicing of uromodulin enhances mitochondrial metabolism for adaptation to stress in kidney epithelial cells.
尿调蛋白的选择性剪接增强了肾脏上皮细胞的线粒体代谢，使其适应压力
Microneedle delivery of CAR-M-like engineered macrophages alleviates intervertebral disc degeneration through enhanced efferocytosis capacity
微针递送CAR-M样工程化巨噬细胞可通过增强胞吞作用缓解椎间盘退变
Endothelial IGFBP6 suppresses vascular inflammation and atherosclerosis
内皮 IGFBP6 抑制血管炎症和动脉粥样硬化
FKBP10 Promotes the Muscle Invasion of Bladder Cancer via Lamin A Dysregulation
FKBP10通过层蛋白A失调促进膀胱癌的肌肉侵袭
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