用户验证的应用
经验证应用
| 项目 | 详情 |
|---|---|
| Positive WB detected in | Calyculin A treated HeLa cells, Calyculin A treated NIH/3T3 cells |
| Positive FC (Intra) detected in | Calyculin A treated NIH/3T3 cells |
| Positive ChIP-qPCR detected in | hTNF-α (30 ng/ml, 1 h) HeLa cells |
推荐稀释比例
- Western Blot (WB)1:2000-1:10000
- Flow Cytometry (FC) (INTRA)0.13 ug per 10^6 cells in a 100 µl suspension
- CHIP-QPCR1:10-1:100
推荐稀释说明
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. Sample-dependent, Check data in validation data gallery.
详细信息
背景信息
Nuclear factor kB (NF-kB) is a collective term for a small family of dimeric transcription factors [comprising p65 (RelA) and RelB, c-Rel, p50/p105 (NF-κB1), and p52/p100 (NF-κB2)]. All NF-κB proteins share a Rel homology domain (RHD), which is responsible for DNA binding and dimerization. Only p65, RelB, and c-Rel contain potent transactivation domains within sequences from the C-terminal to the RHD. Exterior signals lead to the phosphorylation and degradation of the inhibitory complex IκB, which is modulated by the IκB kinase (IKK), and its degradation allows for the release of the typical NF-κB heterodimer, p65/p50, to translocate into the nucleus. NF-κB binds to its cognate DNA elements and can transcriptionally activate different target genes among which 200-500 genes have been implicated in cell survival/apoptosis, cell growth, immune response, and inflammation.
文献引用 (48)
Chimeric antigen receptor with novel intracellular modules improves antitumor performance of T cells
具有新型胞内模块的嵌合抗原受体可提高T细胞的抗肿瘤活性
Nanodelivery of Y-27632 by RGD-modified liposome enhances radioimmunotherapy of hepatocellular carcinoma via tumor microenvironment matrix stiffness reprogramming.
通过RGD修饰的脂质体纳米递送Y-27632，通过肿瘤微环境基质硬度重编程增强肝细胞癌的放射免疫疗法
Macrophage-mimetic liposomes co-delivering ceria and Ac2-26 peptide for penumbra protection in ischemic stroke.
巨噬细胞模拟脂质体共同递送二氧化铈和 Ac2-26 肽，用于缺血性中风的半暗带保护
Circadian disruption and ROS-NLRP3 signaling mediate sleep deprivation-enhanced silica nanoparticle toxicity in lacrimal glands.
昼夜节律紊乱和 ROS-NLRP3 信号传导介导睡眠剥夺增强的二氧化硅纳米颗粒对泪腺的毒性
H3K18 lactylation-mediated nucleotide-binding oligomerization domain-2 (NOD2) expression promotes bilirubin-induced pyroptosis of astrocytes.
H3K18 乳酸化介导的核苷酸结合寡聚化结构域 2 (NOD2) 表达促进胆红素诱导的星形胶质细胞焦亡
ILF3 promotes colorectal cancer cell resistance to ferroptosis by enhancing cysteine uptake and GSH synthesis via stabilizing SLC3A2 mRNA.
ILF3 通过稳定 SLC3A2 mRNA 来增强半胱氨酸的摄取和 GSH 的合成，从而促进结直肠癌细胞对铁死亡的抵抗力
Probiotics alleviate painful diabetic neuropathy by modulating the microbiota-gut-nerve axis in rats
益生菌通过调节大鼠的微生物-肠道-神经轴来缓解糖尿病疼痛性神经病变
JOSD2 alleviates acute kidney injury through deubiquitinating SIRT7 and negativity regulating SIRT7-NF-κB inflammatory pathway in renal tubular epithelial cells.
JOSD2 通过去泛素化 SIRT7 和负调控肾小管上皮细胞中的 SIRT7-NF-κB 炎症通路来减轻急性肾损伤
Tubular CD44 plays a key role in aggravating AKI through NF-κB p65-mediated mitochondrial dysfunction
管状 CD44 通过 NF-κB p65 介导的线粒体功能障碍在加重 AKI 中起关键作用
Migrasomes derived from human umbilical cord mesenchymal stem cells: a new therapeutic agent for ovalbumin-induced asthma in mice
源自人脐带间充质干细胞的迁移体：一种治疗小鼠卵清蛋白诱导哮喘的新型治疗剂
- 1
- 2
- 3
- 4
- 5