GFP tag Polyclonal antibody (50430-2-AP)
eGFP; GFP; CFP; eYFP; GFP-tag
用户验证的应用
经验证应用
| 项目 | 详情 |
|---|---|
| Positive WB detected in | recombinant protein, Transfected HEK-293 cells |
| Positive IP detected in | Transfected HEK-293T cells |
| Positive IF-P detected in | transgenic mouse brain tissue |
| Positive IF/ICC detected in | Transfected HEK-293 cells |
推荐稀释说明
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. Sample-dependent, Check data in validation data gallery.
详细信息
背景信息
Green Fluorescent Proteins (GFPs) encompass a diverse range of proteins carrying a green chromophore, originating from various species and forming different protein lineages. Wildtype GFP consists of 238 amino acid residues (26.9 kDa). GFP was first identified in the jellyfish Aequorea victoria. It emits green light with a peak wavelength of 509 nm upon excitation by blue light at 395 nm. When fused with other proteins, GFP serves as a versatile reporter protein e.g. for quantifying expression levels or facilitates visualization of subcellular localization through fluorescence microscopy. This antibody is a rabbit polyclonal antibody, generated against the full-length eGFP protein. It exhibits reactivity towards variants of Aequorea victoria GFP, including S65T-GFP, RS-GFP, YFP, CFP, and eGFP.
文献引用 (356)
The cryptic lncRNA-encoded microprotein TPM3P9 drives oncogenic RNA splicing and tumorigenesis
隐秘的 lncRNA 编码的微蛋白 TPM3P9 驱动致癌 RNA 剪接和肿瘤发生
Epigenetic silencing of interleukin-10 by host-derived oxidized phospholipids supports a lethal inflammatory response to infections
宿主来源的氧化磷脂通过表观遗传机制沉默白细胞介素-10，从而促进对感染的致命性炎症反应。
Increased TMEM106B levels lead to lysosomal dysfunction which affects synaptic signaling and neuronal health.
TMEM106B 水平升高会导致溶酶体功能障碍，从而影响突触信号传导和神经元健康
KIDINS220 and InsP8 safeguard the stepwise regulation of phosphate exporter XPR1.
KIDINS220 和 InsP8 保障磷酸盐输出因子 XPR1 的逐步调控
Glia detect and transiently protect against dendrite substructure disruption in C. elegans.
神经胶质细胞能够检测并暂时保护秀丽隐杆线虫免受树突亚结构破坏
Shigella effector IpaH1.4 subverts host E3 ligase RNF213 to evade antibacterial immunity.
志贺氏菌效应蛋白 IpaH1.4 抑制宿主 E3 连接酶 RNF213 以逃避抗菌免疫
Interferon-stimulated gene screening identifies CCND3 as a host restriction factor against emerging high-pathogenic bandaviruses.
干扰素刺激基因筛选发现 CCND3 是宿主对抗新出现的高致病性班达病毒的限制因子
CFAP100 couples microtubule glutamylation to spindle pole integrity in keratinocytes to promote epidermal development.
CFAP100 将微管谷氨酰化与角质形成细胞中的纺锤体极完整性联系起来，从而促进表皮发育
Modulation of host ATP levels by secreted bacterial effectors.
细菌分泌效应因子对宿主ATP水平的调节
SWI/SNF ATPase silenced HLF potentiates lung metastasis in solid cancers.
SWI/SNF ATPase 沉默的 HLF 会增强实体瘤的肺转移
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