用户验证的应用
经验证应用
| 项目 | 详情 |
|---|---|
| Positive WB detected in | Transfected HEK-293 cells |
| Positive IP detected in | Transfected HEK-293 cells |
| Positive IF/ICC detected in | Transfected HEK-293 cells |
| Positive FC (Intra) detected in | Transfected HEK-293 cells |
推荐稀释比例
- Western Blot (WB)1:5000-1:50000
- Immunoprecipitation (IP)0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
- Immunofluorescence (IF)/ICC1:200-1:800
- Flow Cytometry (FC) (INTRA)0.25 ug per 10^6 cells in a 100 µl suspension
推荐稀释说明
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. Sample-dependent, Check data in validation data gallery.
详细信息
背景信息
DYKDDDDK Tag ( Equivalent To FLAG Antibody From Sigma ) with the following sequence DYKDDDDK, is a hydrophilic tag for recombinant protein technology. Tags can be used as a tool to localize gene products in a variety of cell types, study proteins topology, and also help to identify and characterize new, low abundance or poorly immunogenic proteins. Due to its high hydrophilic character, the DKYDDDDK tag is likely to be located on the surface of a fusion protein, which enables the tag to be accessible for antibodies. DYKDDDDK Tag Antibody is generated against 1xDYKDDDDK tag (DYKDDDDK) and can recognize protein containing one or more DYKDDDDK tags, independently on N-terminal, C-terminal or internal regions of the target protein. Anti-FLAG is a registered trademark of Sigma-Aldrich Biotechnology.
文献引用 (243)
Low-input redoxomics facilitates global identification of metabolic regulators of oxidative stress in the gut
低输入氧化组学有助于全面识别肠道氧化应激的代谢调节剂
Structural insights into spliceosome fidelity: DHX35-GPATCH1- mediated rejection of aberrant splicing substrates.
剪接体保真度的结构见解：DHX35-GPATCH1介导的对异常剪接底物的排斥
Axin formation inhibitor 1 aggravates hepatic ischemiaâreperfusion injury by promoting the ubiquitination and degradation of PPARβ.
Axin 形成抑制剂 1 通过促进 PPARβ 的泛素化和降解来加重肝脏缺血再灌注损伤
Characterization of dsRNA binding proteins through solubility analysis identifies ZNF385A as a dsRNA homeostasis regulator.
通过溶解度分析对 dsRNA 结合蛋白进行表征，发现 ZNF385A 是 dsRNA 稳态调节因子
Exercise-induced anti-obesity effects in male mice generated by a FOXO1-KLF10 reinforcing loop promoting adipose lipolysis.
通过 FOXO1-KLF10 增强环路促进脂肪分解，从而在雄性小鼠中产生运动诱导的抗肥胖作用
USP19 potentiates autophagic cell death via inhibiting mTOR pathway through deubiquitinating NEK9 in pancreatic cancer.
USP19 通过去泛素化 NEK9 抑制 mTOR 通路，从而增强胰腺癌细胞的自噬性细胞死亡
AKAP1/PKA-mediated GRP75 phosphorylation at mitochondria-associated endoplasmic reticulum membranes protects cancer cells against ferroptosis.
AKAP1/PKA介导的GRP75在与线粒体相关的内质网膜上的磷酸化可保护癌细胞免受铁死亡的影响
USP25 directly interacts with and deubiquitinates PPARα to increase PPARα stability in hepatocytes and attenuate high-fat diet-induced MASLD in mice.
USP25 直接与 PPARα 相互作用并使其去泛素化，从而增加肝细胞中 PPARα 的稳定性，并减轻高脂饮食诱导的小鼠 MASLD
Redox regulation of TRIM28 facilitates neuronal ferroptosis by promoting SUMOylation and inhibiting OPTN-selective autophagic degradation of ACSL4.
TRIM28 的氧化还原调控通过促进 SUMO 化和抑制 OPTN 选择性自噬降解 ACSL4 来促进神经元铁死亡
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