GFP tag Monoclonal antibody (66002-1-Ig)
eGFP; GFP; CFP; eYFP; GFP-tag
用户验证的应用
经验证应用
| 项目 | 详情 |
|---|---|
| Positive WB detected in | GFP transgenic mouse brain tissue, Recombinant protein |
| Positive IP detected in | Transfected HEK-293T cells |
| Positive IF/ICC detected in | Transfected HEK-293 cells |
推荐稀释说明
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. Sample-dependent, Check data in validation data gallery.
详细信息
背景信息
Green Fluorescent Proteins (GFPs) encompass a diverse range of proteins carrying a green chromophore, originating from various species and forming different protein lineages. Wildtype GFP consists of 238 amino acid residues (26.9 kDa). GFP was first identified in the jellyfish Aequorea victoria. It emits green light with a peak wavelength of 509 nm upon excitation by blue light at 395 nm. When fused with other proteins, GFP serves as a versatile reporter protein e.g. for quantifying expression levels or facilitates visualization of subcellular localization through fluorescence microscopy. This antibody is a mouse (IgG2a) monoclonal antibody against GFP reactive to GFP, eGFP, eYFP, YFP and CFP.
文献引用 (245)
The mechanosensitive adhesion G protein-coupled receptor 133 (GPR133/ADGRD1) enhances bone formation.
机械敏感性粘附 G 蛋白偶联受体 133 (GPR133/ADGRD1) 可增强骨形成
TIM3+ breast cancer cells license immune evasion during micrometastasis outbreak
TIM3+乳腺癌细胞在微转移爆发期间促进免疫逃逸
Protein O-GlcNAcylation and hexokinase mitochondrial dissociation drive heart failure with preserved ejection fraction.
蛋白质 O-GlcNAc 糖基化和己糖激酶线粒体解离是射血分数保留型心力衰竭的驱动因素
Genome-wide CRISPR screening identifies LRP1 as an entry factor for SFTSV.
全基因组 CRISPR 筛选发现 LRP1 是 SFTSV 的入侵因子
Combinatorial tagging generates a multi-purpose knock-in mouse model revealing phase separation-dependent germ granules in RNA homeostasis and germline development.
组合标记生成多用途敲入小鼠模型，揭示了 RNA 稳态和生殖细胞发育中依赖于相分离的生殖颗粒
ZNF451 collaborates with RNF8 to regulate RNF168 localization and amplify ubiquitination signaling to promote DNA damage repair and regulate radiosensitivity.
ZNF451 与 RNF8 协同作用，调节 RNF168 的定位，并放大泛素化信号，以促进 DNA 损伤修复和调节放射敏感性
Redox regulation of TRIM28 facilitates neuronal ferroptosis by promoting SUMOylation and inhibiting OPTN-selective autophagic degradation of ACSL4.
TRIM28 的氧化还原调控通过促进 SUMO 化和抑制 OPTN 选择性自噬降解 ACSL4 来促进神经元铁死亡
Deubiquitinase JOSD2 alleviates colitis by inhibiting inflammation via deubiquitination of IMPDH2 in macrophages.
去泛素化酶 JOSD2 通过去泛素化巨噬细胞中的 IMPDH2 来抑制炎症，从而缓解结肠炎
Bunyavirus SFTSV nucleoprotein exploits TUFM-mediated mitophagy to impair antiviral innate immunity
布尼亚病毒 SFTSV 核蛋白利用 TUFM 介导的线粒体自噬来削弱抗病毒先天免疫
The alpha-coronavirus E protein inhibits the JAK-STAT pathway signaling by triggering STAT2 degradation through OPTN- and NBR1-mediated selective autophagy.
甲型冠状病毒 E 蛋白通过 OPTN 和 NBR1 介导的选择性自噬触发 STAT2 降解，从而抑制 JAK-STAT 通路信号传导
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