用户验证的应用
经验证应用
| 项目 | 详情 |
|---|---|
| Positive WB detected in | HeLa cells, U2OS cells, K-562 cells, HEK-293 cells, MCF-7 cells, HepG2 cells, Jurkat cells, L02 cells, Raji cells |
| Positive IP detected in | U2OS cells |
| Positive IHC detected in | human lung cancer tissue, human colon cancer tissue, human liver cancer tissue, human endometrial cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Chloroquine treated HepG2 cells, Starvation treated HepG2 cells, U2OS cells, Chloroquine treated U2OS cells |
推荐稀释说明
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. Sample-dependent, Check data in validation data gallery.
详细信息
背景信息
Sequestosome 1 (SQSTM1/p62) is a multifunctional adaptor protein implicated in selective autophagy, cell signaling pathways, and tumorigenesis. p62 has been implicated in shuttling ubiquitinated and sometimes aggregated proteins for autophagic degradation. As a autophagy-specific substrate, p62 is degraded during the autophagic process, which makes intracellular level of p62 as a marker for autophagy flux. p62 is at the cross-roads of several signaling pathways including Ras/ Raf/ MAPK and NFκB and plays important role in cancer. p62 is a component of inclusion bodies/ protein aggregates found in human diseases, including Huntington's disease, Alzheimer's disease, Parkinson's disease in the brain, and nephropathic cystinosis in kidney (PMID: 22074114, 22860231, 22714671). The molecular weight of p62 is predicted as 48/ 38 kDa, while western blot analyses using this antibody demonstrate the major band around 60-62 kDa in various tissues.
文献引用 (66)
Serine/threonine/tyrosine kinase 1 drives pancreatic carcinogenesis via GSK3β sequestration-mediated Wnt/β-catenin pathway hyperactivation.
丝氨酸/苏氨酸/酪氨酸激酶 1 通过 GSK3β 隔离介导的 Wnt/β-catenin 通路过度激活驱动胰腺癌的发生
Liver-specific gene PGRMC1 blocks c-Myc-induced hepatocarcinogenesis through ER stress-independent PERK activation
肝脏特异性基因PGRMC1通过不依赖于内质网应激的PERK激活来阻断c-Myc诱导的肝癌发生。
Autophagy regulates cellular senescence by mediating the degradation of CDKN1A/p21 and CDKN2A/p16 through SQSTM1/p62-mediated selective autophagy in myxomatous mitral valve degeneration.
自噬通过 SQSTM1/p62 介导的选择性自噬来调节细胞衰老，从而介导 CDKN1A/p21 和 CDKN2A/p16 的降解，进而导致粘液瘤样二尖瓣退化
NAT10 Increases Lysosomal Acidification to Promote Esophageal Cancer Metastasis via ac4C Acetylation of ATP6V0E1 mRNA.
NAT10 通过 ac4C 乙酰化 ATP6V0E1 mRNA 增加溶酶体酸化，从而促进食管癌转移
E2F1-autophagy-ALDH1A1 axis enhances self-renewal and drug resistance of lung cancer stem-like cells in a p53-dependent manner.
E2F1-自噬-ALDH1A1轴以p53依赖的方式增强肺癌干细胞样细胞的自我更新和耐药性
Retromer promotes the lysosomal turnover of mtDNA.
Retromer促进溶酶体对线粒体DNA的降解
Argan Callus Extract Restores Skin Cells via AMPK-Dependent Regulation of Energy Metabolism, Autophagy, and Inflammatory Pathways.
摩洛哥坚果愈伤组织提取物通过 AMPK 依赖性调节能量代谢、自噬和炎症通路来修复皮肤细胞
Heat shock protein 60 manipulates Foot-and-Mouth disease virus replication by regulating mitophagy.
热休克蛋白 60 通过调节线粒体自噬来操纵口蹄疫病毒的复制
Trehalose enhances macrophage autophagy to promote myelin debris clearance after spinal cord injury
海藻糖增强巨噬细胞自噬促进脊髓损伤后髓鞘碎片清除
Chaperonin-containing tailless complex polypeptide 1 subunit 6A negatively regulates autophagy and protects colorectal cancer cells from cisplatin-induced cytotoxicity.
含伴侣蛋白的无尾复合物多肽 1 亚基 6A 负向调节自噬，保护结直肠癌细胞免受顺铂诱导的细胞毒性
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