Anti-Histone H3 (di methyl K9) antibody [mAbcam 1220] - ChIP Grade (Ab1220)

H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, H3C1, H3FC, HIST1H3C, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3K9me2, H3K9me, H3K9

反应种属:Human, Arabidopsis, Bovine, Drosophila, Oryza sativa
抗原名称:H3C1 di methyl K9
应用:WB, IHC, ELISA, IF/ICC, ChIP, Dot

详细信息

免疫原The exact immunogen used to generate this antibody is proprietary information.
宿主/亚型Mouse / IgG2a
抗体类型Monoclonal
产品形式Liquid
纯化方式Affinity purification
储存缓冲液pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 6.97% L-Arginine
储存条件运输条件: Blue Ice 推荐的短期储存时间: 1-2 weeks 推荐的短期储存条件: +4°C 推荐的长期储存条件: -20°C 储存信息: Avoid freeze / thaw cycle
无载体蛋白No

特异性

By peptide ELISA ab1220 recognizes di methyl K9, but not unmodified K9, mono methyl K9, tri methyl K9, di methyl K27, tri methyl K27, mono methyl K4, di methyl K4 or tri methyl K4. By Western blot ab1220 is blocked by di methyl K9, but not by unmodified K9, mono methyl K9, tri methyl K9, di methyl K27, tri methyl K27, mono methyl K4, di methyl K4 or tri methyl K4. This indicates the specificity of ab1220 for di methyl K9 of Histone H3.

背景信息

Anti-Histone H3 (di methyl K9) antibody [mAbcam 1220] - ChIP Grade (ab1220) is a House Mouse Monoclonal antibody and is validated for use in ChIP, Dot, ELISA, ICC/IF, IHC-P, WB in human samples. Anti-Histone H3 (di methyl K9) antibody [mAbcam 1220] - ChIP Grade (ab1220) has been cited over 961 times in peer reviewed journals and is trusted by the scientific community. Abcams high quality validation processes ensure Anti-Histone H3 (di methyl K9) antibody [mAbcam 1220] - ChIP Grade (ab1220) has high sensitivity and specificity. Anti-Histone H3 (di methyl K9) antibody [mAbcam 1220] - ChIP Grade (ab1220) has 71 independent reviews from customers. Anti-Histone H3 (di methyl K9) antibody [mAbcam 1220] - ChIP Grade (ab1220) specifically detects Histone H3 Di Methyl-K9 (UniProt ID: P68431; Molecular weight: 15kDa) and is sold in 100 ug selling sizes. Antibody clone mAbcam 1220 is also available pre-conjugated to a variety of labels for your convenience - HRP, Alexa Fluor® 488, Alexa Fluor® 647 ( ab62168, ab2385, ab23851). Histone H3 (di methyl K9), also known as H3K9me2, is an important epigenetic modification associated with gene silencing and heterochromatin formation. This anti histone H3 antibody detecting H3K9me2 is an essential tool for studying this modification's role in transcriptional repression and chromatin structure. Abnormal H3K9me2 levels are linked to various diseases, including cancer and neurological disorders. Monitoring di methyl K9 modifications can provide valuable insights into disease mechanisms and potential therapeutic targets. Learn about ChIP assay kits, other ChIP antibodies, and more in the ChIP assay guide. ChIP protocols ChIP protocol for cross-linking ChIP (X-ChIP) Native ChIP protocol Chromatin preparation from tissues for ChIP ChIP troubleshooting ChIP tips and tricks guide Want a custom formulation? This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

文献引用 (928)

CRISPR screen of human pancreatic cancer xenografts identifies a KLF5 proliferation vulnerability through epigenetic modifiers NCAPD2 and MTHFD1.

通过 CRISPR 筛选人类胰腺癌异种移植瘤,发现 KLF5 增殖脆弱性与表观遗传修饰因子 NCAPD2 和 MTHFD1 有关。

Maeda Masahiro, Sherman Kenna, Zhou Weiqiang, Cheng Jiaqi, Nihongaki Yuta, Idrizi Adrian, Tryggvadottir Rakel, Camacho Oscar, Shang Xingbo, Min Jimin, Koldobskiy Michael A, Maitra Anirban, Levchenko Andre, Slusher Barbara S, Ji Hongkai, Feinberg Andrew P

CBFA2T2: a novel H3K27 reader regulating metabolism and tumor growth.

CBFA2T2:一种新型的 H3K27 阅读器,可调节代谢和肿瘤生长。

He Shengfei, Ou Yutian, Jiao Fangfang, Feng Jiuxing, Zhu Ping, Ma Jie, Xu Ning, Li Haitao, Guo Rui

Mutation in HER1 enhances stigma exsertion and hybrid seed production in rice.

HER1基因突变可增强水稻柱头伸出和杂交种子生产。

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A SET domain-containing protein and HCF-1 maintain transgenerational epigenetic memory.

SET结构域蛋白和HCF-1维持跨代表观遗传记忆。

G9a-mediated H3K9me2 orchestrates intestinal epithelial regeneration through epigenetic silencing of cell cycle-related genes.

G9a 介导的 H3K9me2 通过表观遗传沉默细胞周期相关基因来调控肠上皮再生。

TP53BP2 Promotes Placental Autophagy and Preeclampsia via G9a and DNMT1 Cooperatively Modulating E2F1.

TP53BP2 通过 G9a 和 DNMT1 协同调节 E2F1 促进胎盘自噬和先兆子痫。

Transposable elements as drivers of genome evolution in Drosophila virilis.

转座元件作为果蝇基因组进化的驱动力。

Rezvykh Alexander P, Kulikova Dina A, Zelentsova Elena S, Protsenko Liudmila, Bespalova Alina V, Guseva Iuliia O, Blumenstiel Justin P, Evgen'ev Mikhail B, Funikov Sergei Y

Developmental stage dominates cell-type identity and reveals a chromatin regulatory function for Rad50 in Drosophila.

发育阶段决定细胞类型特性,并揭示果蝇中 Rad50 的染色质调控功能。

Boutet Thomas, Sakr Rosy, Marzullo Marta, Goyal Manisha, Cattenoz Pierre B, Ciapponi Laura, Mukherjee Tina, Giangrande Angela

Gut microbiota modulation of epigenetic target EHMT2: Lacticaseibacillus rhamnosus Fb7-311 regulated renal cell carcinoma apoptosis and metastasis.

肠道菌群对表观遗传靶标 EHMT2 的调控:鼠李糖乳杆菌 Fb7-311 调节肾细胞癌的凋亡和转移。

Lee Jeongmin, Lee Jinkwon, Tae In Hwan, Kang Yunsang, Kim Jinsan, Ryu Tae Young, Yang Haneol, Lee Su-Gi, Park Kunhyang, Park Doo-Sang, Jung Cho-Rok, Lim Jung Hwa, Lee Moo-Seung, Kim Dae-Soo, Son Mi-Young, Cho Hyun-Soo

Augmented CENH3 loading is accompanied by transcriptional and epigenetic reprogramming at rice centromeres during meiosis.

在水稻减数分裂过程中,CENH3 的增加伴随着着丝粒的转录和表观遗传重编程。

Cao Lei, Zhao Yangzi, You Hanli, Liu Huixin, Wang Bingxin, Run Yonghang, Yang Han, Wen Minsi, Chen Jiawei, Zhao Pengtao, Shen Yi, Li Yafei, Zhang Wenli, Cheng Zhukuan
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