Anti-alpha smooth muscle Actin antibody [EPR5368] (Ab124964)

推荐稀释比例

• IHC-P1/1000 - 1/2500 Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
• WB1/10000 - 1/50000 For unpurified use at 1/1000.
• ICC/IF1/250 - 1/500
• Flow Cyt (Intra)1/100 - 1/200 ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Anti-alpha smooth muscle Actin antibody [EPR5368] (ab124964) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Flow Cyt (Intra), ICC/IF, IHC-P and WB. Anti-alpha smooth muscle Actin antibody [EPR5368] (ab124964) was first used in a scientific publication in 2014 and has been cited over 393 times in peer reviewed journals. It's performance in Western Blot and IHC in human, mouse and rat samples is trusted by the scientific community. Abcam's high quality manufacturing and validation processes ensure Anti-alpha smooth muscle Actin antibody [EPR5368] (ab124964) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility. The specificity of Anti-alpha smooth muscle Actin antibody [EPR5368] (ab124964) has been confirmed by Western Blot testing in alpha smooth muscle Actin knockout HeLa cells. Anti-alpha smooth muscle Actin antibody [EPR5368] (ab124964) has 20 independent reviews from customers. alpha smooth muscle Actin antibodies are often used as loading controls in Western Blot. Anti-alpha smooth muscle Actin antibody [EPR5368] has been verified in Western Blot samples and detects a band at 42kDa Molecular weight. Anti-alpha smooth muscle Actin antibody [EPR5368] (ab124964) specifically detects alpha smooth muscle Actin (UniProt ID: P62736; Molecular weight: 42kDa) and is sold in 100 µL and 1 mL selling sizes. Conjugation-ready, carrier free format available for antibody clone EPR5368 - ab220795. Antibody clone EPR5368 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor ® 488, Alexa Fluor ® 647, Alexa Fluor ® 555, Alexa Fluor ® 594, PE, APC ( ab202295, ab202296, ab202509, ab202510, ab208844, ab225143 ). Alpha-Smooth Muscle Actin (α-SMA) is a critical protein involved in smooth muscle contraction, encoded by the ACTA2 gene. Predominantly found in vascular smooth muscle cells, α-SMA plays a vital role in fibrogenesis and wound healing. It is often used as a marker for myofibroblast formation, which are specialized cells that contribute to tissue repair and fibrosis. The expression of α-SMA is associated with the activation of myofibroblasts, making it an important indicator in studies of fibrosis and other related conditions. Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents. What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies.