Anti-Glutathione Peroxidase 4 antibody [EPNCIR144] (Ab125066)

Phospholipid hydroperoxide glutathione peroxidase GPX4, PHGPx, Glutathione peroxidase 4, GPx-4, GSHPx-4, GPX4

反应种属:Human, Mouse, Rat
抗原名称:Phospholipid hydroperoxide glutathione peroxidase
应用:WB, IHC, FCM, IF/ICC

用户验证的应用

推荐稀释比例

  • IHC-P1/50 - 1/250 Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.
  • WB1/1000 - 1/10000
  • Flow Cyt (Intra)1/400

详细信息

免疫原The exact immunogen used to generate this antibody is proprietary information.
宿主/亚型Rabbit / IgG
抗体类型Monoclonal
全称Anti-Glutathione Peroxidase 4 antibody [EPNCIR144]
产品形式Liquid
储存缓冲液pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
储存条件运输条件: Conditional Ambient 推荐的短期储存时间: 1-2 weeks 推荐的短期储存条件: +4°C 推荐的长期储存条件: -20°C 储存信息: Stable for 12 months at -20°C
纯化方式Affinity purification Protein A

背景信息

Anti-Glutathione Peroxidase 4 antibody [EPNCIR144] (ab125066) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Flow Cyt (Intra), ICC/IF, IHC-P and WB. Anti-Glutathione Peroxidase 4 antibody [EPNCIR144] (ab125066) was first used in a scientific publication in 2012 and has been cited over 567 times in peer reviewed journals. It's performance in Western Blot and IHC in mouse and human samples is trusted by the scientific community. Abcam's high quality manufacturing and validation processes ensure Anti-Glutathione Peroxidase 4 antibody [EPNCIR144] (ab125066) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility. The specificity of Anti-Glutathione Peroxidase 4 antibody [EPNCIR144] (ab125066) has been confirmed by Western Blot testing in Glutathione Peroxidase 4 knockout HeLa cells ( ab262509 ). Anti-Glutathione Peroxidase 4 antibody [EPNCIR144] (ab125066) has 12 independent reviews from customers. Anti-Glutathione Peroxidase 4 antibody [EPNCIR144] (ab125066) specifically detects Glutathione Peroxidase 4 (UniProt ID: O70325; Molecular weight: 22kDa) and is sold in 100 µL and 1 mL selling sizes. Conjugation-ready, carrier free format available for antibody clone EPNCIR144 - ab219592. Antibody clone EPNCIR144 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor ® 488, Alexa Fluor ® 647 ( ab314333, ab314334 ). Top cited antibody for this target with >1000 citations. Glutathione peroxidase 4 is a master regulator of ferroptosis and lipid peroxidation. This antibody is crucial in cancer research, particularly in understanding GPX4's role in cell death and antioxidant defence and is widely used in studies of reactive oxygen species (ROS) and GPX4 inhibitors for therapeutic applications. Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents. What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies.

文献引用 (1522)

Lymph node environment drives FSP1 targetability in metastasizing melanoma.

淋巴结环境驱动转移性黑色素瘤中 FSP1 的靶向性。

Metabolic stress sensing by epithelial RXRα links westernization of diet with Crohn's disease.

上皮细胞 RXRα 对代谢应激的感知将饮食西化与克罗恩病联系起来。

Meyer Moritz, Grabherr Felix, Plattner Christina, Hadjihannas Michel V, Rao Zhigang, Marteau Valentin, López-Agudelo Víctor Alonso, Schwärzler Julian, Mayr Lisa, Jukic Almina, Scheffauer Laura, Zundel Luis, Enrich Barbara, Pfister Alexandra, Simonini Anna, Grander Christoph, Hilbe Richard, Haschka David, Zollner Andreas, Vouk Kathrin, Moser Patrizia, Hess Michael W, Nemati Niloofar, Rieder Dietmar, Sommer Felix, Rosenstiel Philip, Ran Qitao, Blumberg Richard S, Kaser Arthur, Rieder Florian, Koeberle Andreas, Becker Christoph, Atreya Raja, Kühl Anja A, Siegmund Britta, Franke Andre, Tilg Herbert, Trajanoski Zlatko, Adolph Timon E

Chiral metal nanozyme-enabled SOD2 activation and siRNA delivery cooperatively mitigating chronic heart failure.

手性金属纳米酶激活 SOD2 和 siRNA 递送,协同缓解慢性心力衰竭。

Wang Zekun, Zhang Ruofei, Hu Yanyu, Song Qile, Fu Xiaoyan, Wang Yifan, Chen Mei, Fan Kelong, Fan Cundong, Sun Dongdong

GPX4-dependent ferroptosis governs ILC2 homeostasis and colitis progression.

GPX4 依赖性铁死亡控制 ILC2 的稳态和结肠炎的进展。

Liu Gaoyu, Wang Ying, Huang Huachen, Wang Jianye, Yang Jinhao, Wu Jingyi, Cao Hailong, Zhang Yuxin, Jing Yuchao, Zhou Pan, Yu Ying, Chen Chun, Liu Qiang, Zhou Jie

Riboflavin metabolism shapes FSP1-driven ferroptosis resistance.

核黄素代谢塑造了 FSP1 驱动的铁死亡抵抗力。

Skafar Vera, de Souza Izadora, Ghosh Biplab, Ferreira Dos Santos Ancely, Porto Freitas Florencio, Chen Zhiyi, Sun Shibo, Donate Castillo Merce, Nepachalovich Palina, Seufert Lars, Bothe Sebastian, Tschuck Juliane, Mathur Apoorva, Nunes-Alves Ariane, Buhr Jannik, Aponte-Santamaría Camilo, Schmitz Werner, Mack Matthias, Eilers Martin, Bargou Ralf, Chaufan Milena, Kaur Mayher, Palma Mario, Ubellacker Jessalyn M, Elling Ulrich, Augustin Hellmut G, Hadian Kamyar, Meierjohann Svenja, Proneth Bettina, Conrad Marcus, Fedorova Maria, Alborzinia Hamed, Friedmann Angeli José Pedro

Peritumoural adipose tissue promotes ferroptosis resistance by 3-hydroxykynurenine-mediated suppression of ferritinophagy.

肿瘤周围脂肪组织通过 3-羟基犬尿氨酸介导的抑制铁蛋白自噬来促进铁死亡抵抗。

Zhang Yan-Yu, Han Yi, Tan Yue-Tao, Lu Yun-Xin, Ma Meng-Yao, Zheng Yong-Qiang, Chen Hao-Jie, Fu Hai-Yan, Mo Hai-Yu, Wu Qi-Nian, Luo Xiao-Jing, Liao Kun, Chen Wen-Qi, Zeng Zhao-Lei, Piao Hai-Long, Du Hong-Li, Lin Jun-Zhong, Tian Tian, Xu Rui-Hua, Ju Huai-Qiang

Tumour acidosis remodels the glycocalyx to control lipid scavenging and ferroptosis.

肿瘤酸中毒重塑糖萼,以控制脂质清除和铁死亡。

Bång-Rudenstam Anna, Cerezo-Magaña Myriam, Horvath Marton, Talbot Hugo, Gustafsson Emma, Jonathan Stevanus, Chakraborty Chaitali, Nissen Itzel, Gonçalves de Oliveira Kelin, Boukredine Axel, Beyer Sarah, Perez Julio Enriquez, Johansson Maria C, Kjellén Lena, Tykesson Emil, Malmström Anders, van Kuppevelt Toin H, Forsberg-Nilsson Karin, Esko Jeffrey D, Remeseiro Silvia, Bengzon Johan, Governa Valeria, Belting Mattias

Chronic enteritis triggered by diet westernization is driven by epithelial ATG16L1-mediated autophagy.

饮食西化引发的慢性肠炎是由上皮细胞 ATG16L1 介导的自噬驱动的。

Mayr Lisa, Schwärzler Julian, Scheffauer Laura, Rao Zhigang, Rieder Dietmar, Grabherr Felix, Meyer Moritz, Scheler Jakob, Jukic Almina, Zundel Luis, Wieser Verena, Zollner Andreas, Simonini Anna, Auer Stefanie, Amann Lisa, Philipp Maureen, Leierer Johannes, Hilbe Richard, Weiss Günter, Moser Patrizia, Rosenstiel Philip, Ran Qitao, Blumberg Richard S, Kaser Arthur, Koeberle Andreas, Trajanoski Zlatko, Tilg Herbert, Adolph Timon E

Inhibition of Calcium-Dependent Lipid Droplets Relocation of ACSL4-PKCβ-ALOX15 Complex Alleviates Ferroptosis and Acute Pancreatitis.

抑制钙依赖性脂滴 ACSL4-PKCβ-ALOX15 复合物的移位可缓解铁死亡和急性胰腺炎。

Hou Guoyuan, Luan Jing, Xu Xiaoyong, Qin Jianhua, Ma Shuang, He Jiyuan, Sun Na, Zhang Wei, Gao Minghui

TrxR2 Lactylation Facilitates Mitochondrial Protection and Endothelial Ferroptosis Resistance in Diabetic Cardiomyopathy.

TrxR2 乳酸化促进糖尿病心肌病中的线粒体保护和内皮细胞铁死亡抵抗。

Li Su, Liu Muyin, Chen Chao, Li Xinyan, Yan Xiaopei, Zhu Wentao, Qiu Wenyan, Li Qiyu, Sun Xiangyu, Huang Chao, Yin Ming, Chen Zhangwei, Lu Yao, Ge Junbo, Kong Xiangqing, Qian Juying, Chen Yuqiong
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