HRP Anti-GAPDH antibody [mAbcam 9484] - Loading Control (AB9482)

推荐稀释比例

• WB1/1000 - 1/5000 NOT SUITABLE for blocking with milk. Block in 5% BSA for 1 hour. Our labs have thoroughly investigated the blocking conditions for this ab following concerning customer feedback on the lack of signal with some vials. We found that milk significantly decreases signal and is therefore not a suitable blocking agent for this ab (see images of our tests on the datasheet of the unconjugated version, ab9484 ). This change in characteristics of the ab is due to a recent update to the production process.

What is this antibody validated in? HRP Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab9482) is a mouse monoclonal antibody and is validated for use in Western Blot (WB) in Human, Mouse, Rat samples. What is the molecular weight of GAPDH? Anti-GAPDH [mAbcam 9484] - Loading Control HRP (ab9482) is often used as a loading control in Western blot. It specifically detects a band for GAPDH (UniProt: P04406) at a molecular weight of 40.2kDa. Trusted by the scientific community Anti-GAPDH [mAbcam 9484] - Loading Control HRP (ab9482) was first used in a scientific publication in 2004 and has been cited over 220 times in peer-reviewed journals. Reviewed by scientists Anti-GAPDH [mAbcam 9484] - Loading Control HRP (ab9482) has over 15 independent reviews from customers. Related products Antibody clone mAbcam 9484 is also available pre-conjugated to a variety of labels for your convenience – Anti-GAPDH HRP [mAbcam 9484] - Loading Control (ab9482). Other related products We have a range of other formats of antibody clone [mAbcam 9484] also available for your convenience: ab9484, Alexa Fluor® 680 - ab184095, Alexa Fluor® 790 - ab184578, Alexa Fluor® 647 - ab196697