Anti-GAPDH antibody - Loading Control (AB9485)
GAPD, CDABP0047, OK/SW-cl.12, GAPDH, Glyceraldehyde-3-phosphate dehydrogenase, Peptidyl-cysteine S-nitrosylase GAPDH
用户验证的应用
推荐稀释比例
- IHC-P5 µg/mL Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
- WB1/2500 Some customers have experienced that milk significantly decreases the signal in WB compared to BSA. In-house we use BSA. We recommend Goat Anti-Rabbit IgG H&L (Alexa Fluor ® 790) ( ab175781 ) secondary antibody.
详细信息
背景信息
Anti-GAPDH antibody - Loading Control (ab9485) is a rabbit polyclonal antibody and is validated for use in ICC/IF, IHC-P and WB. Anti-GAPDH antibody - Loading Control (ab9485) was first used in a scientific publication in 2004 and has been cited over 3283 times in peer reviewed journals. It's performance in Western blot in human, mouse and rat samples is trusted by the scientific community. Abcam's high quality validation processes ensure Anti-GAPDH antibody - Loading Control (ab9485) has high sensitivity and specificity. Anti-GAPDH antibody - Loading Control (ab9485) has 87 independent reviews from customers. GAPDH antibodies are often used as loading controls in Western Blot. Anti-GAPDH antibody - Loading Control has been verified in Western Blot samples and detects a band at 36kDa Molecular weight. Anti-GAPDH antibody - Loading Control (ab9485) specifically detects GAPDH (UniProt ID: P04406; Molecular weight: 36kDa) and is sold in 100 µg and 250 µg selling sizes. One of the top cited antibody in the market for GAPDH with >4500 citations and >60 five star reviews. GAPDH is a key target involved in glycolysis and cell metabolism. It plays a crucial role as a housekeeping gene, particularly in understanding GAPDH expression and its function as a metabolic enzyme. GAPDH is widely analysed in GAPDH activity assays and studies of its role in various cellular processes. Additionally, GAPDH is commonly used as a loading control in western blotting, IHC and immunofluorescence experiments.
文献引用 (3706)
C/EBPβ activation in vascular smooth muscle cells promotes hyperlipidemia-induced phenotypic transition and arterial stiffness
血管平滑肌细胞中C/EBPβ的激活促进高脂血症诱导的表型转变和动脉僵硬
Microtubules Sequester Acetylated YAP in the Cytoplasm and Inhibit Heart Regeneration
微管将乙酰化的YAP隔离在细胞质中，从而抑制心脏再生
Zongertinib (BI 1810631), an Irreversible HER2 TKI, Spares EGFR Signaling and Improves Therapeutic Response in Preclinical Models and Patients with HER2-Driven Cancers
Zongertinib (BI 1810631) 是一种不可逆的 HER2 TKI，可抑制 EGFR 信号传导并改善临床前模型和 HER2 驱动癌症患者的治疗反应
Targeting PERP promotes anti-tumor immunity in HNSCC by regulating tumor immune microenvironment and metabolic homeostasis
靶向PERP可通过调节肿瘤免疫微环境和代谢稳态促进头颈部鳞状细胞癌的抗肿瘤免疫。
Subaqueous acoustic pressure system based one day heterotypic pseudo-islet spheroid formation with adipose derived stem cells for graft survival-related function enhancement.
基于水下声压系统，利用脂肪来源干细胞在一天内形成异型假胰岛球体，以增强移植存活相关的功能
A natural polyphenolic nanoparticle--knotted hydrogel scavenger for osteoarthritis therapy.
一种用于骨关节炎治疗的天然多酚纳米颗粒——结状水凝胶清除剂
An AI-assisted fluorescence microscopic system for screening mitophagy inducers by simultaneous analysis of mitophagic intermediates.
一种利用人工智能辅助荧光显微镜系统，通过同时分析线粒体自噬中间体来筛选线粒体自噬诱导剂
Unveiling aging heterogeneities in human dermal fibroblasts via nanosensor chemical cytometry.
利用纳米传感器化学细胞计数法揭示人类真皮成纤维细胞的衰老异质性
Ankyrin-B modulates mitochondrial fission in skeletal muscle and is required for optimal endurance exercise capacity.
Ankyrin-B 调节骨骼肌中的线粒体分裂，是获得最佳耐力运动能力所必需的
Major-depressive-disorder-associated dysregulation of ZBTB7A in orbitofrontal cortex promotes astrocyte-mediated stress susceptibility
重度抑郁症相关的眶额皮质中ZBTB7A失调促进星形胶质细胞介导的应激易感性
- 1
- 2
- 3
- 4
- 5
- 6
- 371