Anti-CD31 antibody [JC/70A] (Ab9498)
CD31, Platelet endothelial cell adhesion molecule, PECAM-1, EndoCAM, GPIIA', PECA1, PECAM1
用户验证的应用
推荐稀释比例
- IHC-P0.5 µg/mL The ideal fixation time will depend on the size of the tissue block and the type of tissue, but fixation between 18–24h is suitable for most samples. Positive Control: Hu tonsil tissue Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
- WB1/1000 Treat samples with PNGase F or phosphatase to confirm the specificity of bands if necessary. The observed band size of CD31 may not the same as predicted MWs in WB due to the different forms and modifications of CD31. Hu Isoform 1-6: 79-83 kDa (predicted) Observed band size is around 130 kDa Positive Control: HUVEC and Jurkat cell lysates ( ab7899 ); Human spleen and kidney tissue lysate. Negative Control: NIH/3T3 whole cell lysate ( ab7179 ).
- ICC/IF1 µg/mL Use 0.3M glycine to quench autofluorescence caused by aldehydes.
- Flow Cyt1/20
- IHC-Fr1 µg/mL
详细信息
背景信息
Anti-CD31 antibody [JC/70A] (ab9498) is a mouse monoclonal antibody and is validated for use in Flow Cyt, ICC/IF, IHC-Fr, IHC-P and WB. Abcam's high quality validation processes ensure Anti-CD31 antibody [JC/70A] (ab9498) has high sensitivity and specificity. Anti-CD31 antibody [JC/70A] (ab9498) has 38 independent reviews from customers. Anti-CD31 antibody [JC/70A] (ab9498) specifically detects CD31 (UniProt ID: P16284; Molecular weight: 80kDa) and is sold in 100 ug selling sizes. Conjugation-ready, carrier free format available for antibody clone JC/70A - ab264090. Antibody clone JC/70A is also available pre-conjugated to a variety of labels for your convenience - PE, Alexa Fluor ® 488, Alexa Fluor ® 647 ( ab21566, ab215911, ab215912 ). CD31 (PECAM-1) is a protein expressed on endothelial and immune cells, playing a crucial role in tumor angiogenesis and immune cell trafficking. In immuno-oncology, it influences the tumor microenvironment and modulates the immune response to cancer Want a custom formulation? This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
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