Anti-mouse IgG for IP (HRP) (Ab131368)
Igh-4, Ighg1, Ig gamma-1 chain C region secreted form, Igh-4, Ighg1
用户验证的应用
推荐稀释比例
- WB1/1000 - 1/10000 The optimal dilution will depend on the sensitivity of the HRP substrate.
详细信息
背景信息
Number of blots: At least 20 blots, based on a 1 μl/ml dilution and 5 ml diluted antibody per blot. Important protocol notes: 1. The anti-mouse IgG for IP secondary antibody (HRP) detects mouse IgG antibodies (subtypes: IgG1, IgG2a, IgG2b, IgG3). 2. The anti-mouse IgG for IP secondary antibody (HRP) preferentially detects the non-reduced form of mouse IgG (IgG1, IgG2a, IgG2b, IgG3) over the reduced, SDS-denatured forms. 3. IP sample should be completely reduced/denatured before loaded onto a western blot. 4. Milk should be used as the blocking protein for the immunoblot. Western blot and IP resources: a) Western blot a beginner's guide b) IP protocol c) IP troubleshooting tips
文献引用 (162)
Host-transposon mutualism supports regeneration in planarians.
宿主-转座子互利共生支持涡虫的再生。
Insulin receptor trafficking and interactions in muscle cells.
胰岛素受体在肌肉细胞中的转运和相互作用。
Irinotecan alleviates chemoresistance to anthracyclines through the inhibition of AARS1-mediated BLM lactylation and homologous recombination repair.
伊立替康通过抑制 AARS1 介导的 BLM 乳酸化和同源重组修复来缓解蒽环类药物的耐药性
PEX39 facilitates the peroxisomal import of PTS2-containing proteins.
PEX39 促进含 PTS2 蛋白进入过氧化物酶体
Cleavage of CAD by caspase-3 determines the cancer cell fate during chemotherapy.
化疗期间，caspase-3 对 CAD 的切割决定了癌细胞的命运
Intestinal Epithelial-Derived USP13 Alleviates Colonic Inflammation by Suppressing GRP78-mediated Endoplasmic Reticulum Stress.
肠上皮细胞衍生的 USP13 通过抑制 GRP78 介导的内质网应激来缓解结肠炎症。
Acod1 Promotes PAD4 Ubiquitination via UBR5 Alkylation to Modulate NETosis and Exert Protective Effects in Sepsis.
Acod1 通过 UBR5 烷基化促进 PAD4 泛素化，从而调节 NETosis 并在脓毒症中发挥保护作用。
Ribonuclease 1 Induces T-Cell Dysfunction and Impairs CD8(+) T-Cell Cytotoxicity to Benefit Tumor Growth through Hijacking STAT1.
核糖核酸酶 1 通过劫持 STAT1 诱导 T 细胞功能障碍并损害 CD8(+) T 细胞的细胞毒性，从而促进肿瘤生长
Secreted Clever-1 modulates T cell responses and impacts cancer immunotherapy efficacy.
分泌型 Clever-1 调节 T 细胞反应并影响癌症免疫疗法的疗效
DNA damage response regulator ATR licenses PINK1-mediated mitophagy.
DNA损伤反应调节因子ATR可激活PINK1介导的线粒体自噬
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