Anti-CD4 antibody [EPR6855] (Ab133616)

CD4, T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3

反应种属:Human
抗原名称:CD4
应用:WB, IHC, FCM, IF/ICC

用户验证的应用

推荐稀释比例

  • IHC-P1/500 For unpurified use at 1/100 - 1/250. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.
  • WB1/5000 For unpurified use at 1/1000 - 1/10000.
  • ICC/IF1/100 - 1/250
  • Flow Cyt (Intra)1/100 ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

详细信息

免疫原The exact immunogen used to generate this antibody is proprietary information.
宿主/亚型Rabbit / IgG
抗体类型Monoclonal
全称Anti-CD4 antibody [EPR6855]
产品形式Liquid
储存缓冲液pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
储存条件运输条件: Conditional Ambient 推荐的短期储存时间: 1-2 weeks 推荐的短期储存条件: +4°C 推荐的长期储存条件: -20°C 储存信息: Stable for 12 months at -20°C
纯化方式Affinity purification Protein A

背景信息

Anti-CD4 antibody [EPR6855] (ab133616) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Flow Cyt (Intra), ICC/IF, IHC-P, WB and mIHC. Anti-CD4 antibody [EPR6855] (ab133616) was first used in a scientific publication in 2014 and has been cited over 318 times in peer reviewed journals. It's performance in IHC and Immunofluorescence in human samples is trusted by the scientific community. Abcam's high quality manufacturing and validation processes ensure Anti-CD4 antibody [EPR6855] (ab133616) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility. Anti-CD4 antibody [EPR6855] (ab133616) has 19 independent reviews from customers. Anti-CD4 antibody [EPR6855] (ab133616) specifically detects CD4 (UniProt ID: P01730; Molecular weight: 49kDa) and is sold in a convenient trial size to enable initial testing (20 µL) and larger sizes for subsequent scaling up experiments (100 µL and 1 mL). Conjugation-ready, carrier free format available for antibody clone EPR6855 - ab181724. Antibody clone EPR6855 is also available pre-conjugated to a variety of labels for your convenience - HRP, Alexa Fluor ® 647, Alexa Fluor ® 488, Alexa Fluor ® 594, Alexa Fluor ® 555 ( ab195842, ab196147, ab196372, ab277931, ab280849 ). IL-4 plays a role in conditions like systemic lupus erythematosus (SLE) and multiple sclerosis, where it influences immune responses and inflammation. Specificity and sensitivity confirmed in IHC with multi-tissue microarray (TMA) validation Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents. What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies.

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