Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (Ab133741)

LMN2, LMNB, LMNB1, Lamin-B1

反应种属:Human, Mouse, Rat
抗原名称:LMNB1
应用:WB, IHC, IF/ICC, IP

用户验证的应用

推荐稀释比例

  • IHC-P1/300 Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • WB1/1000 - 1/10000
  • ICC/IF1 µg/mL Recommended for methanol-fixed samples in ICC only. Use ab229025 for compatibility with both methanol and PFA.
  • IP1/20

详细信息

免疫原The exact immunogen used to generate this antibody is proprietary information.
宿主/亚型Rabbit / IgG
抗体类型Monoclonal
全称Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker
产品形式Liquid
储存缓冲液pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
储存条件运输条件: Conditional Ambient 推荐的短期储存时间: 1-2 weeks 推荐的短期储存条件: +4°C 推荐的长期储存条件: -20°C 储存信息: Avoid freeze / thaw cycle
纯化方式Affinity purification Protein A

背景信息

Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in ICC/IF, IHC-P, IP, WB in human, mouse, rat samples. Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741) has been cited over 189 times in peer reviewed journals and is trusted by the scientific community. Abcams high quality manufacturing and validation processes ensure Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility. The specificity of Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741) has been confirmed by testing in knockout samples. Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741) has 14 independent reviews from customers. Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741) specifically detects Lamin B1 (UniProt ID: P20700; Molecular weight: 66kDa) and is sold in a convenient trial size to enable initial testing (20 uL) and larger sizes for subsequent scaling up experiments (100 uL and 1 mL). Conjugation-ready, carrier free format available for antibody clone EPR8985(B) - ab22797. Antibody clone EPR8985(B) is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor ® 488, Alexa Fluor ® 647, HRP, Alexa Fluor ® 594 ( ab19416, ab19418, ab19419, ab216723 ). Lamin B1/LMNB1/LMNB is a critical component of the nuclear lamina, playing a vital role in chromatin organization and nuclear stability. Utilizing our lmnb1 antibody can significantly enhance the detection and study of Lamin B1. Research indicates that mutations in Lamin B1 are linked to demyelinating diseases, such as adult-onset autosomal dominant leukodystrophy (ADLD), highlighting its importance in maintaining neural health Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents. What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies.

文献引用 (223)

NOX4-derived oxidative DNA damage impairs thyroid differentiation through an epigenetic mechanism in BRAF-mutated radioactive iodine refractory papillary thyroid cancer cells.

NOX4 衍生的氧化性 DNA 损伤通过表观遗传机制损害 BRAF 突变的放射性碘难治性乳头状甲状腺癌细胞的甲状腺分化。

Radom Mickaëlle, Buffet Camille, Cazarin Juliana, Harinquet Marylin, de Faria Caroline Coelho, Brayé Floriane, Nobre Catline, Aglave Marine, Mesloub Yasmina, Dayris Thibault, Droin Nathalie, Godefroy Karine, Bani Mohamed-Amine, Ghuzlan Abir Al, Leboulleux Sophie, Lamartina Livia, Dupuy Corinne

Protease-mediated PRC1 dissociation promotes H2AK119ub remodeling during stress responses.

蛋白酶介导的 PRC1 解离促进应激反应期间 H2AK119ub 的重塑。

Cui Wei, Li Qingyang, Wei Jinsong, Zhou Ting, Zhu Haozhe, Huang Delai, Wang Shuai, Gao Jianan, Zhou Ru, Sun Zeyu, Ruan Hua, Lo Li Jan, Tao Ting, Chen Jun, Peng Jinrong, Shi Hui

Chromosome length is constrained by spindle scaling to ensure faithful mitosis in mammals.

染色体长度受到纺锤体比例的限制,以确保哺乳动物有丝分裂的正常进行。

Zhao Yu-Long, Zhao Yi-Ming, Yan Yi-Fang, Yang Ning, Ma Si-Nan, Wang Rui-Jia, Feng Gui-Hai, Li Zhi-Kun, Li Wei, Wang Li-Bin

The chromatin remodeller CHD4 regulates transcription factor binding to both prevent activation of silent enhancers and maintain active regulatory elements.

染色质重塑因子 CHD4 调节转录因子结合,既能防止沉默增强子的激活,又能维持活性调控元件。

Koulle Andria, Ogundele Oluwaseun, Shah Devina, Baker India, Lopez Maya, Lando David, Reynolds Nicola, Ragheb Ramy, Laue Ernest D, Hendrich Brian

Hops (Humulus lupulus) Extract Enhances Redox Resilience and Attenuates Quinolinic Acid-Induced Excitotoxic Damage in the Brain.

啤酒花(Humulus lupulus)提取物增强氧化还原抵抗力,并减轻喹啉酸引起的脑部兴奋性毒性损伤。

Ortega Daniela Ramírez, Hernández Pérez Erick R, Gutiérrez Magdaleno Montserrat, Meza-Sosa Karla F, Pineda Calderas Lucia, Álvarez Silva María José, Vázquez Cervantes Gustavo I, González Esquivel Dinora F, González Alfonso Wendy Leslie, Navarro Cossio Javier Angel, Ovalle Rodríguez Paulina, Flores Itamar, Salazar Alelí, Gómez-Manzo Saul, Pineda Benjamín, Pérez de la Cruz Verónica

Clinical significance and molecular mechanism of CDX2-CBX3 regulatory axis in lung adenocarcinoma progression.

CDX2-CBX3调控轴在肺腺癌进展中的临床意义及分子机制。

Liu Shicheng, Zhao Qingtao, Ren Dahu, Kong Lingxin, Zhao Hongzhen, Duan Guochen

Fetal hepatocytes protect the HSPC genome via fetuin-A

胎肝细胞通过胎球蛋白A保护造血干细胞基因组。

Xiao-Lin Guo # ,Yi-Ding Wang # ,Yan-Jun Liu # ,Lei Chu ,Hua Zhu ,Ye Hu ,Ren-Yan Wu ,Hong-Yu Xie ,Juan Yu ,Shui-Ping Li ,Chao-Yang Xiong ,Ruo-Yan Li ,Fang Ke ,Lei Chen ,Guo-Qiang Chen ,Liang Chen ,Fan Bai ,Tariq Enver ,Guo-Hong Li ,Huai-Fang Li ,Deng-Li Hong

Transferrin receptor 1 nuclear translocation facilitates tumor progression via p53-mediated chromatin interactions and genome-wide alterations

转铁蛋白受体1核转位通过p53介导的染色质相互作用和全基因组改变促进肿瘤进展

Yaxin Hou # ,Guoheng Tang # ,Qizhi Wang ,Meng Zhou ,Ran Xu ,Xuehui Chen ,Guizhi Shi ,Zhuoran Wang ,Xiyun Yan ,Jie Zhuang ,Kelong Fan

Transposable element expression and sub-cellular dynamics during hPSC differentiation to endoderm, mesoderm, and ectoderm lineages.

hPSC分化为内胚层、中胚层和外胚层谱系过程中转座元件的表达和亚细胞动态

Babarinde Isaac A, Fu Xiuling, Ma Gang, Li Yuhao, Liang Zhangting, Xu Jianfei, Xiao Zhen, Qiao Yu, Lin Zheng, Oleynikova Katerina, Akinwole Mobolaji T, Zhou Xuemeng, Ruzov Alexey, Hutchins Andrew P

Spatially-restricted inflammation-induced senescent-like glia in multiple sclerosis and patient-derived organoids

多发性硬化症和患者来源的类器官中空间受限的炎症诱导的衰老样胶质细胞

Francesca Fagiani # ,Edoardo Pedrini # ,Maria Sofia Martire ,Gaia Gastoldi ,Colin Vanden Bulcke ,Jing-Ping Lin ,Dragan Maric ,Elena Brambilla ,Francesca Ruffini ,Carolina Peri ,Peter A Calabresi ,Pietro Maggi ,Paola Panina-Bordignon ,Gianvito Martino ,Daniel S Reich ,Martina Absinta
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