Anti-Iba1 antibody [EPR16589] - Microglia marker - Mouse IgG1 (Chimeric) (Ab283319)

推荐稀释比例

• IHC-P1/10000 Fluorescence detection system is not guaranteed in IHC‑P application. The mouse and rat recommendation is based on the Immunohistochemistry results. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
• WB1/1000
• ICC/IF1/100
• Flow Cyt (Intra)1/10000
• IP1/30

Find all reagents to label astrocytes in our " Microglia markers guide ". Anti-Iba1 antibody [EPR16589] - Mouse IgG1 (Chimeric) (ab283319) is a mouse monoclonal recombinant antibody validated for use in IHC-P, IP, WB, ICC/IF and Flow Cyt (Intra) in mouse, rat and human samples. Clone EPR16589 was initially developed by Abcam using patented rabbit monoclonal antibody technology and has been engineered to generate this mouse chimeric antibody which retains the binding properties of the RabMAb parent, but has a mouse Fc region. By necessity, some rabbit sequence is retained as part of the variable domain. When multiplexing with other rabbit-derived antibodies, using cross absorbed Fc-reactive secondary antibodies are recommended. Abcams high quality manufacturing and validation processes ensure Anti-Iba1 antibody [EPR16589] - Mouse IgG1 (Chimeric) (ab283319) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility. Anti-Iba1 antibody [EPR16589] - Mouse IgG1 (Chimeric) (ab283319) specifically detects Iba1 (AIF-1) (UniProt ID: P55008; Molecular weight: 17kDa) and is sold in a convenient trial size to enable initial testing (20 µL) and larger sizes for subsequent scaling up experiments (100 µL and 1 mL). Conjugation-ready, carrier free format is also available for Anti-Iba1 antibody [EPR16589] - Mouse IgG1 (Chimeric) - ab312913. Iba1 (Ionized calcium-binding adapter molecule 1; allograft inflammatory factor 1, AIF1) is a prominent microglial marker extensively used in neuroscience research. Its expression increases in various neurodegenerative conditions, including Alzheimer's disease (AD), indicating microglial activation and neuroinflammation. Researchers utilize Iba1 in stainings to assess microglial response and activity in different models, making it essential for understanding inflammatory processes in the brain. Changes in Iba1 expression provide valuable insights into neuroinflammation, highlighting its importance in studying diseases like AD. This mouse monoclonal chimeric antibody has been engineered from a RabMAb parent antibody ( ab178847 ). By necessity, some rabbit sequence is retained as part of the variable domain. When multiplexing with other rabbit-derived antibodies, using cross absorbed Fc-reactive secondary antibodies are recommended. What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies.