用户验证的应用
经验证应用
| 项目 | 详情 |
|---|---|
| WB | 1:500-1:2000 |
| IP | IP:1-5 μL/mg of lysate |
推荐稀释说明
Optimal concentrations/dilutions should be determined by the end user.
详细信息
特异性
Human Cdk6
制备方法
Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the human Cdk6, and purified by antigen affinity chromatography.
运输条件
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
背景信息
The cell-cycle kinase CDK6 has been recognized not only as a typical cyclin-dependent kinase but as a transcriptional regulator with properties distinct from those of its close homologue CDK4. CDK6 regulates the transcription of a number of genes and its effects may be dependent on or independent of its kinase activity. The transcriptional function of CDK6 is crucial for its role in promoting myeloid and lymphoid malignancies, including AML and ALL and is important in maintaining hematopoietic and leukemic stem cells. CDK6 contributes to tumor formation by regulating transcriptional responses in a stage-specific manner. In early stages CDK6 kinase induces a complex transcriptional program to block p53 in hematopoietic cells. Cells lacking CDK6 kinase function are required to mutate p53 to achieve a fully transformed immortalized state. CDK6 binds to the promoters of genes including the p53-antagonists PRMT5, PPM1D and MDM4. References Bellutti F, et al. (2018) Cdk6 antagonizes p53-induced responses during tumorigenesis. Cancer Discov 8 (7): 884-897.