Anti-Ki67 antibody (Ab15580)

Proliferation marker protein Ki-67, Antigen identified by monoclonal antibody Ki-67, Antigen KI-67, Antigen Ki67, MKI67

反应种属:Human, Mouse
抗原名称:MKI67
应用:IHC, IF/ICC

用户验证的应用

推荐稀释比例

  • IHC-P0.1-5 µg/mL Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.
  • ICC/IF0.5-1 µg/mL If fixing cells in 4% PFA (20 min, room temp), it is recommended to permeabilized cells with 0.1% Triton-X for 5 min. Positive Control: HeLa and HAP1 cells

详细信息

免疫原The exact immunogen used to generate this antibody is proprietary information.
宿主/亚型Rabbit / IgG
抗体类型Polyclonal
全称Anti-Ki67 antibody
产品形式Liquid
储存缓冲液pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA
储存条件运输条件: Blue Ice 推荐的短期储存时间: 1-2 weeks 推荐的短期储存条件: +4°C 推荐的长期储存条件: -20°C 储存信息: Avoid freeze / thaw cycle
纯化方式Affinity purification Immunogen

背景信息

Anti-Ki67 antibody (ab15580) is a rabbit polyclonal antibody and is validated for use in ICC/IF and IHC-P. Anti-Ki67 antibody (ab15580) was first used in a scientific publication in 1970 and has been cited over 4218 times in peer reviewed journals. It's performance in Western blot, immunofluorescence and IHC in human and mouse samples is trusted by the scientific community. Abcam's high quality validation processes ensure Anti-Ki67 antibody (ab15580) has high sensitivity and specificity. The specificity of Anti-Ki67 antibody (ab15580) has been confirmed by ICC/IF testing in Ki67 knockout HAP1 cells. Anti-Ki67 antibody (ab15580) has 223 independent reviews from customers. Anti-Ki67 antibody (ab15580) specifically detects Ki67 (UniProt ID: P46013; Molecular weight: 359kDa) and is sold in 100ug selling sizes. Ki-67 is a protein marker that indicates cell proliferation, with higher levels indicating more aggressive and rapidly growing tumors. It is used in cancer diagnostics to assess tumor behaviour, guide treatment decisions and predict prognosis based on the rate of tumor cell division.

文献引用 (5338)

PKC-eta promotes breast cancer metastasis by regulating the Hippo-YAP signaling pathway.

PKC-eta 通过调节 Hippo-YAP 信号通路促进乳腺癌转移。

Liju Vijayasteltar B, Waidha Kamran, Muraleedharan Amitha, Jayaram Divya Ram, Haimov Hodaya, Jagadeeshan Sankar, Manikandan Dinesh Babu, Shareb Raghda Abu, Nurukurti Livingstone, Sklarz Menachem, Gutkind J Silvio, Allon Irit, Cohen Ofir, Elkabets Moshe, Livneh Etta

Epigenetic memory of colitis promotes tumour growth.

结肠炎的表观遗传记忆促进肿瘤生长。

Nagaraja Surya, Ojeda-Miron Lety, Zhang Ruochi, Oreskovic Ena, Hock Conrad, Hu Yan, Zeve Daniel, Sharma Karina, Hyman Roni R, Zhang Qiming, Castillo Andrew, Breault David T, Yilmaz Ömer H, Buenrostro Jason D

The origin of hepatocellular carcinoma depends on metabolic zonation.

肝细胞癌的起源取决于代谢分区。

Male-biased Yap1-Cd276/B7-H3 axis for immune evasion in medulloblastoma.

男性偏向的 Yap1-Cd276/B7-H3 轴用于髓母细胞瘤的免疫逃避。

Abdelfattah Nourhan, Natarajan Sivaraman, Tran Han Nhat, Wong Thomas, Faisal Maryam, Maldonado Jose, McMinimy Rachael, Borland Hannah, Chen Shu-Hsia, Zhao Hong, Vasquez Matthew, Rodriguez Freddys F, Wagner Carston R, Camargo Fernando, Olson James, George Joshy, Yun Kyuson

Hepatic adaptation to chronic metabolic stress primes tumorigenesis.

肝脏对慢性代谢应激的适应会促进肿瘤发生。

Tzouanas Constantine N, Shay Jessica E S, Sherman Marc S, Rubin Adam J, Mead Benjamin E, Dao Tyler T, Tao Junyan, Lehrich Brandon M, Eng George, Patterson-Fortin Jeffrey, Butzlaff Titus, Mana Miyeko D, Kolb Kellie E, Walesky Chad, Pepe-Mooney Brian J, Smith Colton J, Prakadan Sanjay M, Ramseier Michelle L, Tong Yuzhou Evelyn, Joung Julia, Chi Fangtao, McMahon-Skates Thomas, Winston Carolyn L, Jeong Woo-Jeong, Aney Katherine J, Chen Ethan, Nissim Sahar, Zhang Feng, Deshpande Vikram, Monga Satdarshan P, Lauer Georg M, Goessling Wolfram, Yilmaz Ömer H, Shalek Alex K

CBFA2T2: a novel H3K27 reader regulating metabolism and tumor growth.

CBFA2T2:一种新型的 H3K27 阅读器,可调节代谢和肿瘤生长。

He Shengfei, Ou Yutian, Jiao Fangfang, Feng Jiuxing, Zhu Ping, Ma Jie, Xu Ning, Li Haitao, Guo Rui

Rebalancing NTRK2 isoforms promotes vascular regeneration in bronchopulmonary dysplasia.

NTRK2 同工型的重新平衡可促进支气管肺发育不良中的血管再生。

Zhang Yunpei, Tan Cheng, Liu Ziyi, Mao Xiangdi, Jiang Cheng, Mohammed Afzaal Nadeem, Li Xiaolei, Lu Renzhong, Wang Anmin, Maihemuti Wusiman, Pek Nicole, Fu Hailu, Milbes Omar, Pandrangi Kavya, Johnson Colin Patrick, Sekar Varun, Liu Yaping, Lai Li, Pryhuber Gloria S, Kalinichenko Vladimir V, Miao Yifei, Guo Minzhe, Gu Mingxia

Chiral metal nanozyme-enabled SOD2 activation and siRNA delivery cooperatively mitigating chronic heart failure.

手性金属纳米酶激活 SOD2 和 siRNA 递送,协同缓解慢性心力衰竭。

Wang Zekun, Zhang Ruofei, Hu Yanyu, Song Qile, Fu Xiaoyan, Wang Yifan, Chen Mei, Fan Kelong, Fan Cundong, Sun Dongdong

Stromal vascular fraction self-assembles vascularized osteogenic organoids with immunomodulatory functions.

基质血管成分可自组装形成具有免疫调节功能的血管化成骨类器官。

Wu Jiazhou, He Ying, Qian Tao, Liu Zexian, Wu Yanbin, Li Yazhou, Jiang Hongyu, Ye Jianting, Lv Jia, Ma Biao, Luo Endong, You Jialiang, Wang Dingkai, Bai Yun, Zhang Junming, Zuo Liang, Peng Jiang

On-demand mild photothermal cascade platform reprogramming mitochondrial immunity for tendon rejuvenation.

按需温和光热级联平台重编程线粒体免疫以实现肌腱再生。

Zheng Zitian, Hu Yichen, Zhu Yucheng, Zhang Hanchen, Yang Meng, Ding Guocheng, Wu Yang, Yang Fan, Lu Boyun, Zhou Zheng, Liu Xiaojun, Zhang Guanxin, Zhang Xin, Zhang Deqing, Wang Jianquan, Huang Hongjie
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