SARS-CoV/SARS-CoV-2 Nucleocapsid Antibody, Mouse MAb (40143-MM05)

经验证应用

应用说明

(Antibody's applications have not been validated with corresponding viruses. Optimal concentrations/dilutions should be determined by the end user.)

推荐稀释说明

Optimal concentrations/dilutions should be determined by the end user.

特异性

SARS-CoV Nucleocapsid Has cross-reactivity in ELISA and WB with SARS-CoV-2 Nucleocapsid Protein (Cat# 40588-V08B) Has cross-reactivity in ELISA with SARS-CoV-2 Omicron (B.1.1.529) Nucleocapsid Protein (Cat# 40588-V07E34) SARS-CoV-2 Omicron (BA.2) Nucleocapsid Protein (Cat# 40588-V07E35) SARS-CoV-2 Omicron XE(BA.1 x BA.2) Nucleocapsid Protein (Cat# 40588-V07E36) SARS-CoV-2 Omicron (BA.4) Nucleocapsid Protein (Cat# 40588-V07E37) SARS-CoV-2 Delta (B.1.617) Nucleocapsid (D377Y) Protein (Cat# 40588-V07E16) SARS-CoV-2 Delta (B.1.617) Nucleocapsid (R203M, D377Y) Protein (Cat# 40588-V07E20) SARS-CoV-2 Delta (B.1.617.2) Nucleocapsid Protein (Cat# 40588-V07E29) SARS-CoV-2 Delta (B.1.617.3) Nucleocapsid (P67S, R203M, D377Y) Protein (Cat# 40588-V07E30) SARS-CoV-2 Alpha (B.1.1.7) Nucleocapsid(R203K, G204R) Protein (Cat# 40588-V07E1) SARS-CoV-2 Alpha (B.1.1.7) Nucleocapsid(I292T) Protein (Cat# 40588-V07E4) SARS-CoV-2 Alpha (B.1.1.7) Nucleocapsid(D3L, R203K, G204R, S235F) Protein (Cat# 40588-V07E7) SARS-CoV-2 Alpha (B.1.1.7) Nucleocapsid(D3L, S235F) Protein (Cat# 40588-V07E8) SARS-CoV-2 Alpha(B.1.1.7/ B.1.237) Nucleocapsid(S194L) Protein (Cat# 40588-V07E3) SARS-CoV-2 Alpha/beta(B.1.1.7/ B.1.351/ A.2.2) Nucleocapsid(P13L) Protein (Cat# 40588-V07E2) SARS-CoV-2 Beta(B.1.351/B.1.351.2/b.1.351.3/B.1.427/B.1.429) Nucleocapsid (T205I) Protein (Cat# 40588-V07E9) SARS-CoV-2 Gamma(P.1/ P.1.1/ P.1.2) Nucleocapsid (P80R) Protein (Cat# 40588-V07E11) SARS-CoV-2 Eta(B.1.525) Nucleocapsid (A12G, T205I) Protein (Cat# 40588-V07E19) SARS-CoV-2 Nucleocapsid Protein (Cat# 40588-V07E) No cross-reactivity in ELISA with MERS-CoV Nucleocapsid protein (40068-V08B) HCoV-229E Nucleocapsid protein (40640-V07E) HCoV-NL63 Nucleocapsid protein (40641-V07E) HCoV-HKU1(isolate N5) Nucleocapsid protein (40642-V07E) HCoV-OC43 Nucleocapsid protein (40643-V07E)

制备方法

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant SARS-CoV Nucleoprotein / NP (Catalog#40143-V08B; NP_828858.1; Met1-Ala422). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

运输条件

This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. The coronavirus N protein is required for coronavirus RNA synthesis and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is the most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to the formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of the N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool. References 1.Van Boheemen S, et al. (2012), MBio. 3(6):e00473-12. Bisht H. et al., 2004, Proc Natl Acad Sci. 101 (17): 6641-6. Li W. et al., 2005, Science. 309 (5742): 1864-8.