用户验证的应用
经验证应用
| 项目 | 详情 |
|---|---|
| ELISA | 1:5000-1:10000 |
应用说明
IHC, FCM, IF, IP et al. applications haven't been validated. (Antibody's applications haven't been validated with corresponding virus positive samples. Optimal concentrations/dilutions should be determined by the end user.)
推荐稀释说明
Optimal concentrations/dilutions should be determined by the end user.
详细信息
特异性
SARS-CoV-2 Spike S1 Protein SARS-CoV-2 Spike RBD Protein SARS-CoV Spike S1 Protein SARS-CoV Spike RBD Protein Has cross-reactivity in ELISA with SARS-CoV-2 Omicron (LP.8.1) Spike S1+S2 trimer Protein (Cat# 40589-V08H69) SARS-CoV-2 Omicron (KP.3.1.1) Spike S1+S2 trimer Protein (Cat# 40589-V08H63) SARS-COV-2 Omicron (KP.3) Spike S1+S2 trimer Protein (Cat# 40589-V08H64) SARS-CoV-2 (LP.8.1) Spike RBD Protein (Cat# 40592-V08H160) SARS-CoV-2 (LF.7) Spike RBD Protein (Cat# 40592-V08H159) SARS-CoV-2 (XEC) Spike S1+S2 trimer Protein (Cat# 40589-V08H62) SARS-COV-2 Omicron (KP.2) Spike S1+S2 trimer Protein (Cat# 40589-V08H61) SARS-COV-2 Omicron (KP.2) Spike RBD Protein (Cat# 40592-V08H156) SARS-COV-2 Omicron (KP.3) Spike RBD Protein (Cat# 40592-V08H157) SARS-CoV-2 Omicron (XBB.1.16) Spike S1+S2 trimer Protein (Cat# 40589-V08H48) SARS-CoV-2 Omicron (XBB.1.5) Spike RBD Protein (Cat# 40592-V08H146) SARS-CoV-2 Omicron (XBB.1.5) Spike S1+S2 trimer Protein (Cat# 40589-V08H45) SARS-CoV-2 Omicron (XBB.1) Spike S1+S2 trimer Protein (Cat# 40589-V08H42) SARS-CoV-2 Omicron (XBB) Spike RBD Protein (Cat# 40592-V08H144) SARS-CoV-2 Omicron (BQ.1.1) Spike S1+S2 trimer Protein (Cat# 40589-V08H41) SARS-CoV-2 Omicron (BQ.1.1) Spike RBD Protein (Cat# 40592-V08H143) SARS-CoV-2 Omicron (BF.7) Spike S1+S2 trimer Protein (Cat# 40589-V08H39) SARS-CoV-2 Omicron (BA.4.6/BF.7) Spike RBD Protein (Cat# 40592-V08H140) SARS-CoV-2 Omicron (BA.4.6) Spike S1+S2 trimer Protein (Cat# 40589-V08H37) SARS-CoV-2 Omicron (BA.2.75) Spike RBD Protein (Cat# 40592-V08H135) SARS-CoV-2 Omicron (BA.2.75) Spike S1+S2 trimer Protein (Cat# 40589-V08H36) SARS-CoV-2 Omicron (BA.2.75.2) Spike RBD Protein (Cat# 40592-V08H141) SARS-CoV-2 Omicron (BA.2.75.2) Spike S1+S2 trimer Protein (Cat# 40589-V08H38) SARS-CoV-2 Omicron (BA.2.3.20) Spike RBD Protein (Cat# 40592-V08H142) SARS-CoV-2 Omicron (BA.1.1) Spike S1+S2 trimer Protein (Cat# 40589-V08H29) SARS-CoV-2 Omicron (BA.1.1) Spike RBD Protein (Cat# 40592-V08H129) SARS-CoV-2 Omicron (B.1.1.529) S1+S2 trimer Protein (Cat# 40589-V08H26) SARS-CoV-2 Omicron (B.1.1.529) Spike S1 Protein (Cat# 40591-V08H41) SARS-CoV-2 Omicron (B.1.1.529) Spike RBD Protein (Cat# 40592-V08H121) SARS-CoV-2 Omicron (BA.2) Spike S1+S2 trimer Protein (Cat# 40589-V08H28) SARS-CoV-2 Omicron (BA.2) Spike S1 Protein (Cat# 40591-V08H43) SARS-CoV-2 Omicron (BA.2) Spike RBD Protein (Cat# 40592-V08H123) SARS-CoV-2 Omicron (BA.2.12.1) Spike RBD Protein (Cat# 40592-V08H132) SARS-CoV-2 Omicron (BA.4) Spike RBD Protein (Cat# 40592-V08H130) SARS-CoV-2 XD(BA.1 x AY.4) Spike S1+S2 trimer Protein (Cat# 40589-V08H30) SARS-CoV-2 Delta (B.1.617.2) Spike S1+S2 trimer Protein (Cat# 40589-V08H10) SARS-CoV-2 Delta (B.1.617.2) Spike S1+S2 Protein (Cat# 40589-V08B16) SARS-CoV-2 Delta (B.1.617.2) Spike S1 Protein (Cat# 40591-V08H23) SARS-CoV-2 Delta (B.1.617.2) Spike RBD Protein (Cat# 40592-V08H90) SARS-CoV-2 Spike S1 Protein (Cat# 40591-V08H) Medium cross-reactivity in ELISA with SARS-CoV-2 Omicron (BA.5) Spike RBD Protein (Cat# 40592-V08H131) No cross-reactivity in ELISA with SARS-CoV-2 Omicron (BA.2) Spike S1 NTD Protein (Cat# 40591-V08H44) SARS-CoV-2 Delta (B.1.617.2) Spike S1 NTD Protein (Cat# 40591-V08H36) MERS-CoV Spike S1 Protein (Cat# 40069-V08B1) HCoV-HKU1 (isolate N1) Spike S1 Protein (Cat# 40021-V08H) HCoV-HKU1 (isolate N5) Spike S1 Protein (Cat# 40602-V08H) HCoV-NL63 Spike S1 Protein (Cat# 40600-V08H) HCoV-229E Spike S1 Protein (Cat# 40601-V08H) HCoV-OC43 Spike S1+S2 ECD Protein (Cat# 40607-V08B)
制备方法
This product is a recombinant monoclonal antibody expressed from HEK293 cells.
运输条件
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
背景信息
The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. Known receptors bind S1 are ACE2 (angiotensin-converting enzyme 2), DPP4 (dipeptidyl peptidase-4), APN (aminopeptidase N), CEACAM (carcinoembryonic antigen-related cell adhesion molecule), Sia (sialic acid), O-ac Sia (O-acetylated sialic acid). The spike is essential for both host specificity and viral infectivity. The term 'peplomer' is typically used to refer to a grouping of heterologous proteins on the virus surface that function together. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. It's been reported that SARS-CoV-2 (COVID-19 coronavirus, 2019-nCoV) can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. The main functions for the Spike protein are summarized as: Mediate receptor binding and membrane fusion; Defines the range of the hosts and specificity of the virus; Main component to bind with the neutralizing antibody; Key target for vaccine design; Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD), leading to a higher mortality rate. References Shen S, et al . (2007) Expression, glycosylation, and modification of the spike (S) glycoprotein of SARS CoV. Methods Mol Biol. 379: 127-35. Du L, et al . (2009) The spike protein of SARS-CoV--a target for vaccine and therapeutic development. Nat Rev Microbiol. 7 (3): 226-36. Xiao X, et al . (2004) The SARS-CoV S glycoprotein. Cell Mol Life Sci. 61 (19-20): 2428-30.
文献引用 (96)
Human protein interaction networks of ancestral and variant SARS-CoV-2 in organ-specific cells and bodily fluids.
人类蛋白质相互作用网络：SARS-CoV-2 祖先型和变异型病毒在器官特异性细胞和体液中的相互作用
Chimeric receptor-binding domain vaccine design and sequential immunization enhanced broadly neutralizing antibody responses against COVID-19.
嵌合受体结合域疫苗设计和序贯免疫增强了针对 COVID-19 的广谱中和抗体反应
Papaverine Targets STAT Signaling: A Dual-Action Therapy Option Against SARS-CoV-2.
罂粟碱靶向 STAT 信号通路：对抗 SARS-CoV-2 的双重作用疗法选择
Efficacy of parainfluenza virus 5 (PIV5)-vectored intranasal COVID-19 vaccine as a single dose primer and booster against SARS-CoV-2 variants.
副流感病毒 5 (PIV5) 载体鼻内 COVID-19 疫苗作为单剂量启动剂和加强剂对抗 SARS-CoV-2 变种的功效
Deletion of the Envelope gene attenuates SARS-CoV-2 infection by altered Spike localization and increased cell-to-cell transmission.
包膜基因的缺失通过改变刺突蛋白的定位和增加细胞间传播来减弱 SARS-CoV-2 感染
Trivalent recombinant protein vaccine induces cross-neutralization against XBB lineage and JN.1 subvariants: preclinical and phase 1 clinical trials
三价重组蛋白疫苗可诱导针对 XBB 谱系和 JN.1 亚型的交叉中和作用：临床前和 I 期临床试验
Battery-free, wireless, and electricity-driven soft swimmer for water quality and virus monitoring
用于水质和病毒监测的无电池、无线、电力驱动软游泳器
Single spike mutation differentiating XBB.1 and XBB.1.5 enhances SARS-CoV-2 cell-to-cell transmission and facilitates serum-mediated enhancement
区分 XBB.1 和 XBB.1.5 的单个刺突突变增强了 SARS-CoV-2 细胞间传播，并促进了血清介导的增强
Spatial immune landscapes of SARS-CoV-2 gastrointestinal infection: macrophages contribute to local tissue inflammation and gastrointestinal symptoms
SARS-CoV-2 胃肠道感染的空间免疫景观：巨噬细胞导致局部组织炎症和胃肠道症状
Development of pseudotyped VSV-SARS-CoV-2 spike variants for the assessment of neutralizing antibodies
开发用于评估中和抗体的假型VSV-SARS-CoV-2刺突蛋白变体
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